2'-Hydroxy-4'-methylacetophenone

Cat. No.: HY-34204 Purity: 99.47%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

2'-Hydroxy-4'-methylacetophenone, a phenolic compound isolated from Angelicae koreana roots possesses acaricidal property. It could be used in the preparation of 4’-methyl-2’-[(p-tolylsulfonyl) oxy] acetophenone.

For research use only. We do not sell to patients.

2'-Hydroxy-4'-methylacetophenone Chemical Structure

CAS No. : 6921-64-8

Size	Price	Stock	Quantity
Liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 83	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 83	In-stock	Estimated Time of Arrival: December 31
Liquid
5 g	USD 75	Get quote	Estimated Time of Arrival: December 31
10 g	USD 130	Get quote	Estimated Time of Arrival: December 31
25 g	USD 260	Get quote	Estimated Time of Arrival: December 31
50 g		Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

2'-Hydroxy-4'-methylacetophenone, a phenolic compound isolated from Angelicae koreana roots possesses acaricidal property^[1]. It could be used in the preparation of 4’-methyl-2’-[(p-tolylsulfonyl) oxy] acetophenone^[2].

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	> 1 x 10^-4 M Compound: 29	Cytotoxicity against human A549 cells after 72 hrs by XTT assay Cytotoxicity against human A549 cells after 72 hrs by XTT assay	[PMID: 20619940]
HeLa	IC₅₀	> 1 x 10^-4 M Compound: 29	Cytotoxicity against human HeLa cells after 72 hrs by XTT assay Cytotoxicity against human HeLa cells after 72 hrs by XTT assay	[PMID: 20619940]
HL-60	IC₅₀	> 1 x 10^-4 M Compound: 29	Cytotoxicity against human HL60 cells after 72 hrs by XTT assay Cytotoxicity against human HL60 cells after 72 hrs by XTT assay	[PMID: 20619940]
HT-29	IC₅₀	> 1 x 10^-4 M Compound: 29	Cytotoxicity against human HT-29 cells after 72 hrs by XTT assay Cytotoxicity against human HT-29 cells after 72 hrs by XTT assay	[PMID: 20619940]

Molecular Weight

150.17

Formula

C₉H₁₀O₂

CAS No.

6921-64-8

Appearance

Liquid (Density: 1.08 g/cm³)

Color

Colorless to light yellow

SMILES

CC(C1=CC=C(C)C=C1O)=O

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Pure form	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (665.91 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	6.6591 mL	33.2956 mL	66.5912 mL
5 mM	1.3318 mL	6.6591 mL	13.3182 mL
10 mM	0.6659 mL	3.3296 mL	6.6591 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (16.65 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (16.65 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (16.65 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.47%

Select Batch:

Data Sheet (273 KB) SDS (393 KB)

COA (251 KB) HNMR (131 KB) GC (204 KB)

Handling Instructions (2659 KB)

References

[1]. Oh MS, et al. Acaricidal toxicity of 2'-hydroxy-4'-methylacetophenone isolated from Angelicae koreana roots and structure-activity relationships of its derivatives. J Agric Food Chem. 2012 Apr 11;60(14):3606-11. [Content Brief]

[2]. De Meyer N, et al. 4'-Hydroxy-3-methoxyflavones with potent antipicornavirus activity. J Med Chem. 1991 Feb;34(2):736-46. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	6.6591 mL	33.2956 mL	66.5912 mL	166.4780 mL
	5 mM	1.3318 mL	6.6591 mL	13.3182 mL	33.2956 mL
	10 mM	0.6659 mL	3.3296 mL	6.6591 mL	16.6478 mL
	15 mM	0.4439 mL	2.2197 mL	4.4394 mL	11.0985 mL
	20 mM	0.3330 mL	1.6648 mL	3.3296 mL	8.3239 mL
	25 mM	0.2664 mL	1.3318 mL	2.6636 mL	6.6591 mL
	30 mM	0.2220 mL	1.1099 mL	2.2197 mL	5.5493 mL
	40 mM	0.1665 mL	0.8324 mL	1.6648 mL	4.1619 mL
	50 mM	0.1332 mL	0.6659 mL	1.3318 mL	3.3296 mL
	60 mM	0.1110 mL	0.5549 mL	1.1099 mL	2.7746 mL
	80 mM	0.0832 mL	0.4162 mL	0.8324 mL	2.0810 mL
	100 mM	0.0666 mL	0.3330 mL	0.6659 mL	1.6648 mL