3,3'-Diiodo-L-thyronine (Synonyms: 3,3'-T2)

Name: 3,3'-Diiodo-L-thyronine
Brand: MedChemExpress
SKU: HY-129974
Rating: 4.5 (1 reviews)

Cat. No.: HY-129974 Purity: 97.01%: FAQs
Data Sheet SDS COA Handling Instructions

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3,3'-Diiodo-L-thyronine (3,3'-T2) is an endogenous metabolite of thyroid hormone. 3,3'-Diiodo-L-thyronine significantly enhances COX activity.

For research use only. We do not sell to patients.

3,3'-Diiodo-L-thyronine Chemical Structure

CAS No. : 4604-41-5

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 81	In-stock	Estimated Time of Arrival: December 31
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10 mM * 1 mL in DMSO	USD 81	In-stock	Estimated Time of Arrival: December 31
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5 mg	USD 70	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 110	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 230	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 370	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 600	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of 3,3'-Diiodo-L-thyronine:

3,3'-Diiodo-L-thyronine-¹³C₆ Get quote

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View All COX Isoform Specific Products:

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COX COX-1 COX-2 COX-3

Biological Activity
Purity & Documentation
References
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Description

3,3'-Diiodo-L-thyronine (3,3'-T2) is an endogenous metabolite of thyroid hormone. 3,3'-Diiodo-L-thyronine significantly enhances COX activity^[1]^[2].

IC₅₀ & Target^[2]

Human Endogenous Metabolite

COX

In Vitro

3,3'-Diiodo-L-thyronine (3,3'-T2; 1 μM; 30 min) significantly enhances COX activity^[2].
3,3'-Diiodo-L-thyronine of 1 μM has the maximum effect^[2].
3,3'-Diiodo-L-thyronine is produced by further degradation of T3 and rT3^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

525.08

Formula

C₁₅H₁₃I₂NO₄

CAS No.

4604-41-5

Appearance

Solid

Color

White to off-white

SMILES

N[C@@H](CC1=CC=C(OC2=CC=C(O)C(I)=C2)C(I)=C1)C(O)=O

Structure Classification

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (190.45 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.9045 mL	9.5224 mL	19.0447 mL
5 mM	0.3809 mL	1.9045 mL	3.8089 mL
10 mM	0.1904 mL	0.9522 mL	1.9045 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.76 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (4.76 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (4.76 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 97.01%

Select Batch:

Data Sheet (271 KB) SDS (393 KB)

COA (245 KB) RP-HPLC (252 KB)

Handling Instructions (2659 KB)

References

[1]. Lorenzini L, et al. Assay of Endogenous 3,5-diiodo-L-thyronine (3,5-T2) and 3,3'-diiodo-L-thyronine (3,3'-T2) in Human Serum: A Feasibility Study. Front Endocrinol (Lausanne). 2019 Feb 19;10:88. [Content Brief]

[2]. Lanni A, et al. Rapid stimulation in vitro of rat liver cytochrome oxidase activity by 3,5-diiodo-L-thyronine and by 3,3'-diiodo-L-thyronine. Mol Cell Endocrinol. 1994 Feb;99(1):89-94. [Content Brief]

[3]. Chen X, et al. Simultaneous quantification of T4, T3, rT3, 3,5-T2 and 3,3'-T2 in larval zebrafish (Danio rerio) as a model to study exposure to polychlorinated biphenyls. Biomed Chromatogr. 2018 Jun;32(6):e4185. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.9045 mL	9.5224 mL	19.0447 mL	47.6118 mL
	5 mM	0.3809 mL	1.9045 mL	3.8089 mL	9.5224 mL
	10 mM	0.1904 mL	0.9522 mL	1.9045 mL	4.7612 mL
	15 mM	0.1270 mL	0.6348 mL	1.2696 mL	3.1741 mL
	20 mM	0.0952 mL	0.4761 mL	0.9522 mL	2.3806 mL
	25 mM	0.0762 mL	0.3809 mL	0.7618 mL	1.9045 mL
	30 mM	0.0635 mL	0.3174 mL	0.6348 mL	1.5871 mL
	40 mM	0.0476 mL	0.2381 mL	0.4761 mL	1.1903 mL
	50 mM	0.0381 mL	0.1904 mL	0.3809 mL	0.9522 mL
	60 mM	0.0317 mL	0.1587 mL	0.3174 mL	0.7935 mL
	80 mM	0.0238 mL	0.1190 mL	0.2381 mL	0.5951 mL
	100 mM	0.0190 mL	0.0952 mL	0.1904 mL	0.4761 mL

3,3'-Diiodo-L-thyronine Related Classifications

Metabolic Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

3,3'-Diiodo-L-thyronine4604-41-53,3'-T2Endogenous MetaboliteCOXCyclooxygenasemetabolitethyroidhormoneInhibitorinhibitorinhibit

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3,3'-Diiodo-L-thyronine (Synonyms: 3,3'-T2)

Customer Review

Other Forms of 3,3'-Diiodo-L-thyronine:

3,3'-Diiodo-L-thyronine Related Antibodies

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All COX Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

3,3'-Diiodo-L-thyronine Related Antibodies

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Dilution Calculator

Complete Stock Solution Preparation Table

3,3'-Diiodo-L-thyronine Related Classifications

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