3-Methylcarbazole (Synonyms: NSC 10154)

Cat. No.: HY-W047478 Purity: 97.78%: FAQs
Data Sheet SDS COA Handling Instructions

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3-Methylcarbazole is an carbazole alkaloid compound with anticancer effects. 3-Methylcarbazole shows growth inhibitory activity (IC₅₀ of 25 μg/mL) on human fibrosarcoma HT-1080 cells.

For research use only. We do not sell to patients.

3-Methylcarbazole Chemical Structure

CAS No. : 4630-20-0

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10 mM * 1 mL in DMSO ready for reconstitution	USD 55	In-stock	Estimated Time of Arrival: December 31
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10 mM * 1 mL in DMSO	USD 55	In-stock	Estimated Time of Arrival: December 31
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500 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
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Description

3-Methylcarbazole is an carbazole alkaloid compound with anticancer effects. 3-Methylcarbazole shows growth inhibitory activity (IC₅₀ of 25 μg/mL) on human fibrosarcoma HT-1080 cells^[1].

Molecular Weight

181.23

Formula

C₁₃H₁₁N

CAS No.

4630-20-0

Appearance

Solid

Color

White to light brown

SMILES

CC1=CC2=C(C=C1)NC3=C2C=CC=C3

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (551.79 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.5179 mL	27.5893 mL	55.1785 mL
5 mM	1.1036 mL	5.5179 mL	11.0357 mL
10 mM	0.5518 mL	2.7589 mL	5.5179 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (13.79 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 97.78%

Select Batch:

Data Sheet (276 KB) SDS (393 KB)

COA (254 KB) HNMR (241 KB) LCMS (87 KB)

Handling Instructions (2659 KB)

References

[1]. Cheng-Bin Cui, et al. Carbazole alkaloids as new cell cycle inhibitor and apoptosis inducers from Clausena dunniana Levl. J Asian Nat Prod Res. 2002 Dec;4(4):233-41. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	5.5179 mL	27.5893 mL	55.1785 mL	137.9463 mL
	5 mM	1.1036 mL	5.5179 mL	11.0357 mL	27.5893 mL
	10 mM	0.5518 mL	2.7589 mL	5.5179 mL	13.7946 mL
	15 mM	0.3679 mL	1.8393 mL	3.6786 mL	9.1964 mL
	20 mM	0.2759 mL	1.3795 mL	2.7589 mL	6.8973 mL
	25 mM	0.2207 mL	1.1036 mL	2.2071 mL	5.5179 mL
	30 mM	0.1839 mL	0.9196 mL	1.8393 mL	4.5982 mL
	40 mM	0.1379 mL	0.6897 mL	1.3795 mL	3.4487 mL
	50 mM	0.1104 mL	0.5518 mL	1.1036 mL	2.7589 mL
	60 mM	0.0920 mL	0.4598 mL	0.9196 mL	2.2991 mL
	80 mM	0.0690 mL	0.3449 mL	0.6897 mL	1.7243 mL
	100 mM	0.0552 mL	0.2759 mL	0.5518 mL	1.3795 mL

3-Methylcarbazole Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

3-Methylcarbazole4630-20-0NSC 10154NSC10154NSC-10154OthersInhibitorinhibitorinhibit

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