3-Morpholinosydnonimine (Synonyms: SIN-1; Linsidomine)

Cat. No.: HY-126849: FAQs
Data Sheet Handling Instructions

Molarity Calculator

3-Morpholinosydnonimine (SIN-1) is a spontaneous ROS/RNS generator and peroxynitrite donor. 3-Morpholinosydnonimine induces oxidative stress-induced chondrocyte apoptosis and necrosis. 3-Morpholinosydnonimine inhibits the migration, proliferation and neointima formation of vascular smooth muscle cells by inhibiting the expression of annexin A2. In addition, low doses of 3-Morpholinosydnonimine shows protective effects on Zn²⁺ treated nerve cells.

For research use only. We do not sell to patients.

3-Morpholinosydnonimine Chemical Structure

CAS No. : 33876-97-0

Size		Stock
MOQ		Minimum order quantity
50 mg		Get quote
100 mg		Get quote
250 mg		Get quote
Other size		Get quote

Synthetic products have potential research and development risk.

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Other Forms of 3-Morpholinosydnonimine:

Linsidomine hydrochloride Get quote

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

View All Annexin A Isoform Specific Products:

View All Isoforms

AnxA2

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[4]

AnxA2

In Vitro

3-Morpholinosydnonimine (0-5 mM; 0-24 h) reduces the activity of hypertrophic chondrocytes, induces hypertrophic chondrocyte necrosis and apoptosis of non-hypertrophic ATDC5 and C28/I2 cells^[1].
3-Morpholinosydnonimine (30-1000 μM; 190 min) prevents Zn²⁺ induced neuronal death, but is eliminated by uric acid (HY-B2130)^[2].
3-Morpholinosydnonimine (100-300 μM; 0-190 min) increases the production of reactive oxygen species and active nitrogen, inhibits the Zn²⁺ induced glutathione reductase inactivation and the increase of oxidized glutathione/total glutathione ratio in PC12 cells^[2].
3-Morpholinosydnonimine (1 mM; 2-3 h) induces nuclear accumulation of Nrf2 in EAhy926 and HUVECs cells^[3].
3-Morpholinosydnonimine (0-500 μM; 30 min) inhibits platelet-derived growth factor BB-induced cell migration and proliferation by annexin A2 in rat aortic smooth muscle cells^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	Hypertrophic chondrocytes
Concentration:	0, 1, 2, 3, 4, 5 mM; 5 mM
Incubation Time:	24 h; 0, 4, 8, 12 and 24 h
Result:	Reduced cellular viability of hypertrophic chondrocytes in a dose-dependent manner. Reduced cellular viability of hypertrophic chondrocytes in a time-dependent manner.

Cell Viability Assay^[2]

Cell Line:	differentiated PC12 cells
Concentration:	30, 100, 300 and 1000 μM
Incubation Time:	190 min
Result:	Blocked Zn²⁺-induced cell death in a concentration-dependent manner. Had cytotoxic at higher concentrations than 1 mM.

In Vivo

3-Morpholinosydnonimine (0.01-0.1 mg/kg; 14 days) attenuates neointima formation in balloon-injured rat carotid arteries^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Six-week-old male Sprague Dawley rats (180-190 g) with a balloon-injured carotid artery model^[4]
Dosage:	0.01 and 0.1 mg/kg/day
Administration:	14 days
Result:	Reduced the I/M (intima to media) ratio of the carotid artery in the group treated with 0.1 mg/kg/day. Did not change the I/M ratio of the carotid artery in the group treated with 0.01 mg/kg/day.

Molecular Weight

170.17

Formula

C₆H₁₀N₄O₂

CAS No.

33876-97-0

SMILES

[NH-]C1=C[N+](N2CCOCC2)=NO1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. He Y, et al. 3-morpholinosydnonimine (SIN-1)-induced oxidative stress leads to necrosis in hypertrophic chondrocytes in vitro. Biomed Pharmacother. 2018 Oct;106:1696-1704. [Content Brief]

[2]. An JM, et al. Neuroprotective effect of 3-morpholinosydnonimine against Zn²⁺-induced PC12 cell death. Eur J Pharmacol. 2015 Feb 5;748:37-44. [Content Brief]

[3]. Mattart L, et al. The peroxynitrite donor 3-morpholinosydnonimine activates Nrf2 and the UPR leading to a cytoprotective response in endothelial cells. Cell Signal. 2012 Jan;24(1):199-213. [Content Brief]

[4]. Won KJ, et al. 3-morpholinosydnonimine participates in the attenuation of neointima formation via inhibition of annexin A2-mediated vascular smooth muscle cell migration. Proteomics. 2011 Jan;11(2):193-201. [Content Brief]

3-Morpholinosydnonimine Related Classifications

Molarity Calculator
Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass		Concentration		Volume		Molecular Weight *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)	×	Volume _(start)	=	Concentration _(final)	×	Volume _(final)
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Your Recently Viewed Products:

Product Name

Salutation

Applicant Name *

Email Address *

Phone Number *

Organization Name *

Department *

Requested quantity *

Country or Region *

Remarks