(3S,4S)-A2-32-01

Name: (3S,4S)-A2-32-01
Brand: MedChemExpress
SKU: HY-111532B
Rating: 4.5 (1 reviews)

Cat. No.: HY-111532B Purity: 98.15%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

(3S,4S)-A2-32-01 (compound 24(S,S)), a less active (S,S)-enantiomer of A2-32-01. A2-32-01 is a potent caseinolytic protein proteases (ClpP) inhibitor.

For research use only. We do not sell to patients.

(3S,4S)-A2-32-01 Chemical Structure

Size	Price	Stock	Quantity
Oil + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 495	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 495	In-stock	Estimated Time of Arrival: December 31
Oil
5 mg	USD 450	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 750	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 2250	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 3350	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

or Bulk Inquiry

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This product is a controlled substance and not for sale in your territory.

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Based on 1 publication(s) in Google Scholar

Other Forms of (3S,4S)-A2-32-01:

(3R,4R)-A2-32-01 Get quote

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Biological Activity
Purity & Documentation
References
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Description

(3S,4S)-A2-32-01 (compound 24(S,S)), a less active (S,S)-enantiomer of A2-32-01. A2-32-01 is a potent caseinolytic protein proteases (ClpP) inhibitor^[1].

Molecular Weight

301.42

Formula

C₁₉H₂₇NO₂

Appearance

Oil

Color

Colorless to light yellow

SMILES

O=C1O[C@@H](CCC2=CC=CN=C2)[C@@H]1CCCCCCCC=C

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (331.76 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.3176 mL	16.5882 mL	33.1763 mL
5 mM	0.6635 mL	3.3176 mL	6.6353 mL
10 mM	0.3318 mL	1.6588 mL	3.3176 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 1.25 mg/mL (4.15 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 1.25 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.25 mg/mL (4.15 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 1.25 mg/mL (4.15 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.15%

Select Batch:

Data Sheet (270 KB) SDS (251 KB)

COA (247 KB) HNMR (266 KB) RP-HPLC (166 KB) LCMS (240 KB)

Handling Instructions (2659 KB)

References

[1]. Malte Gersch, et al. The mechanism of caseinolytic protease (ClpP) inhibition. Angew Chem Int Ed Engl. 2013 Mar 4;52(10):3009-14. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.3176 mL	16.5881 mL	33.1763 mL	82.9407 mL
	5 mM	0.6635 mL	3.3176 mL	6.6353 mL	16.5881 mL
	10 mM	0.3318 mL	1.6588 mL	3.3176 mL	8.2941 mL
	15 mM	0.2212 mL	1.1059 mL	2.2118 mL	5.5294 mL
	20 mM	0.1659 mL	0.8294 mL	1.6588 mL	4.1470 mL
	25 mM	0.1327 mL	0.6635 mL	1.3271 mL	3.3176 mL
	30 mM	0.1106 mL	0.5529 mL	1.1059 mL	2.7647 mL
	40 mM	0.0829 mL	0.4147 mL	0.8294 mL	2.0735 mL
	50 mM	0.0664 mL	0.3318 mL	0.6635 mL	1.6588 mL
	60 mM	0.0553 mL	0.2765 mL	0.5529 mL	1.3823 mL
	80 mM	0.0415 mL	0.2074 mL	0.4147 mL	1.0368 mL
	100 mM	0.0332 mL	0.1659 mL	0.3318 mL	0.8294 mL

(3S,4S)-A2-32-01 Related Classifications

Infection

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

(3S,4S)-A2-32-01ClpPCaseinolytic protease PInhibitorinhibitorinhibit

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(3S,4S)-A2-32-01

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Other Forms of (3S,4S)-A2-32-01:

(3S,4S)-A2-32-01 Related Antibodies

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•Related Screening Libraries:

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•Related Proteins:

Biological Activity

Purity & Documentation

References

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Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

(3S,4S)-A2-32-01 Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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SAFETY DATA SHEET (SDS)

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