4′-Demethylnobiletin

Name: 4′-Demethylnobiletin
Brand: MedChemExpress
SKU: HY-142066
Rating: 4.5 (1 reviews)

Cat. No.: HY-142066 Purity: 99.78%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

4′-Demethylnobiletin is a bioactive metabolite that activates the PKA/ERK/CREB signaling pathway, enhances CRE-mediated transcription in hippocampal neurons, and reverses memory impairment associated with NMDA receptor antagonism by stimulating ERK signaling.

For research use only. We do not sell to patients.

4′-Demethylnobiletin Chemical Structure

CAS No. : 34810-62-3

Size	Price	Stock	Quantity
1 mg	USD 500	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 980	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 1560	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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ERK ERK1 ERK2 ERK5 ERK8

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AMPA Receptor NMDA Receptor Kainate Receptor

Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
HL-60	IC₅₀	47.41 μM Compound: XIII, 4'-demethylnobiletin	Antiproliferative activity against HL60 after 24 hrs Antiproliferative activity against HL60 after 24 hrs	[PMID: 17391969]

In Vitro

4′-Demethylnobiletin (1-100 μM, 0-60 min) can activate the phosphorylation of ERK and CREB in rat hippocampal neurons in a time- and concentration-dependent manner, and in a PKA/MEK/ERK pathway-dependent manner, It can also stimulate CRE-mediated transcription by activating PKA/MEK/ERK-dependent signaling pathways^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

4′-Demethylnobiletin (10 or 50 mg/kg, ip, once daily for seven consecutive days) dose-dependently reverses MK-801-induced learning impairment in male ddY mice without affecting the mice's mobility . Mice treated with MK-801 shows less freezing than control mice and induced inhibition of ERK learning activation in the hippocampus of mice, which is also reversed by 4′-Demethylnobiletin^[1].
4′-Demethylnobiletin (10 or 50 mg/kg, ip, once daily for seven consecutive days) reverses the inhibition of MK-801 on NMDA-stimulated phosphorylation of ERK and PKA substrates in hippocampal neurons^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

388.37

Formula

C₂₀H₂₀O₈

CAS No.

34810-62-3

Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C1C=C(C2=CC=C(O)C(OC)=C2)OC3=C(OC)C(OC)=C(OC)C(OC)=C13

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (257.49 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.5749 mL	12.8743 mL	25.7486 mL
5 mM	0.5150 mL	2.5749 mL	5.1497 mL
10 mM	0.2575 mL	1.2874 mL	2.5749 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (6.44 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: 2.5 mg/mL (6.44 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.78%

Select Batch:

Data Sheet (275 KB) SDS (251 KB)

COA (251 KB) HNMR (154 KB) RP-HPLC (161 KB)

Handling Instructions (2659 KB)

References

[1]. Md Al Rahim, et al. 4'-Demethylnobiletin, a bioactive metabolite of nobiletin enhancing PKA/ERK/CREB signaling, rescues learning impairment associated with NMDA receptor antagonism via stimulation of the ERK cascade. Biochemistry. 2009 Aug 18;48(32):7713-21. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.5749 mL	12.8743 mL	25.7486 mL	64.3716 mL
	5 mM	0.5150 mL	2.5749 mL	5.1497 mL	12.8743 mL
	10 mM	0.2575 mL	1.2874 mL	2.5749 mL	6.4372 mL
	15 mM	0.1717 mL	0.8583 mL	1.7166 mL	4.2914 mL
	20 mM	0.1287 mL	0.6437 mL	1.2874 mL	3.2186 mL
	25 mM	0.1030 mL	0.5150 mL	1.0299 mL	2.5749 mL
	30 mM	0.0858 mL	0.4291 mL	0.8583 mL	2.1457 mL
	40 mM	0.0644 mL	0.3219 mL	0.6437 mL	1.6093 mL
	50 mM	0.0515 mL	0.2575 mL	0.5150 mL	1.2874 mL
	60 mM	0.0429 mL	0.2146 mL	0.4291 mL	1.0729 mL
	80 mM	0.0322 mL	0.1609 mL	0.3219 mL	0.8046 mL
	100 mM	0.0257 mL	0.1287 mL	0.2575 mL	0.6437 mL