1. Metabolic Enzyme/Protease
  2. Ser/Thr Protease
  3. 4-Nitrophenyl 4-guanidinobenzoate hydrochloride

4-Nitrophenyl 4-guanidinobenzoate hydrochloride  (Synonyms: NPGB hydrochloride)

Cat. No.: HY-137814 Purity: 99.38%
COA Handling Instructions

4-Nitrophenyl 4-guanidinobenzoate (NPGB) hydrochloride is a potent sperm acrosin inhibitor, and also a trypsin substrate.

For research use only. We do not sell to patients.

4-Nitrophenyl 4-guanidinobenzoate hydrochloride Chemical Structure

4-Nitrophenyl 4-guanidinobenzoate hydrochloride Chemical Structure

CAS No. : 19135-17-2

Size Price Stock Quantity
Free Sample (0.1 - 0.5 mg)   Apply Now  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
USD 60 In-stock
Solution
10 mM * 1 mL in DMSO USD 60 In-stock
Solid
25 mg USD 55 In-stock
50 mg USD 90 In-stock
100 mg USD 150 In-stock
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Description

4-Nitrophenyl 4-guanidinobenzoate (NPGB) hydrochloride is a potent sperm acrosin inhibitor, and also a trypsin substrate[1].

Molecular Weight

336.73

Formula

C14H13ClN4O4

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(OC1=CC=C([N+]([O-])=O)C=C1)C2=CC=C(NC(N)=N)C=C2.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (296.97 mM; ultrasonic and warming and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.9697 mL 14.8487 mL 29.6974 mL
5 mM 0.5939 mL 2.9697 mL 5.9395 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: 5 mg/mL (14.85 mM); Clear solution; Need ultrasonic

    This protocol yields a clear solution of 5 mg/mL.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 5 mg/mL (14.85 mM); Clear solution; Need ultrasonic

    This protocol yields a clear solution of 5 mg/mL.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.9697 mL 14.8487 mL 29.6974 mL 74.2435 mL
5 mM 0.5939 mL 2.9697 mL 5.9395 mL 14.8487 mL
10 mM 0.2970 mL 1.4849 mL 2.9697 mL 7.4243 mL
15 mM 0.1980 mL 0.9899 mL 1.9798 mL 4.9496 mL
20 mM 0.1485 mL 0.7424 mL 1.4849 mL 3.7122 mL
25 mM 0.1188 mL 0.5939 mL 1.1879 mL 2.9697 mL
30 mM 0.0990 mL 0.4950 mL 0.9899 mL 2.4748 mL
40 mM 0.0742 mL 0.3712 mL 0.7424 mL 1.8561 mL
50 mM 0.0594 mL 0.2970 mL 0.5939 mL 1.4849 mL
60 mM 0.0495 mL 0.2475 mL 0.4950 mL 1.2374 mL
80 mM 0.0371 mL 0.1856 mL 0.3712 mL 0.9280 mL
100 mM 0.0297 mL 0.1485 mL 0.2970 mL 0.7424 mL
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4-Nitrophenyl 4-guanidinobenzoate hydrochloride Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
4-Nitrophenyl 4-guanidinobenzoate hydrochloride
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