4-Nitrophenyl 4-guanidinobenzoate hydrochloride (Synonyms: NPGB hydrochloride)

Cat. No.: HY-137814 Purity: 99.38%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

4-Nitrophenyl 4-guanidinobenzoate (NPGB) hydrochloride is a potent sperm acrosin inhibitor, and also a trypsin substrate.

For research use only. We do not sell to patients.

4-Nitrophenyl 4-guanidinobenzoate hydrochloride Chemical Structure

CAS No. : 19135-17-2

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 60	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 60	In-stock	Estimated Time of Arrival: December 31
Solid
25 mg	USD 55	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 90	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 150	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Purity & Documentation
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Description

4-Nitrophenyl 4-guanidinobenzoate (NPGB) hydrochloride is a potent sperm acrosin inhibitor, and also a trypsin substrate^[1].

Molecular Weight

336.73

Formula

C₁₄H₁₃ClN₄O₄

CAS No.

19135-17-2

Appearance

Solid

Color

White to off-white

SMILES

O=C(OC1=CC=C([N+]([O-])=O)C=C1)C2=CC=C(NC(N)=N)C=C2.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (296.97 mM; ultrasonic and warming and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.9697 mL	14.8487 mL	29.6974 mL
5 mM	0.5939 mL	2.9697 mL	5.9395 mL
10 mM	0.2970 mL	1.4849 mL	2.9697 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 5 mg/mL (14.85 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 5 mg/mL (14.85 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: 5 mg/mL (14.85 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 5 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.38%

Select Batch:

Data Sheet (269 KB) SDS (252 KB)

COA (246 KB) HNMR (137 KB) RP-HPLC (138 KB) LCMS (108 KB)

Handling Instructions (2659 KB)

References

[1]. J M Kaminski, et al. Synthesis and inhibition of human acrosin and trypsin and acute toxicity of aryl 4-guanidinobenzoates. J Med Chem. 1986 Apr;29(4):514-9. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.9697 mL	14.8487 mL	29.6974 mL	74.2435 mL
	5 mM	0.5939 mL	2.9697 mL	5.9395 mL	14.8487 mL
	10 mM	0.2970 mL	1.4849 mL	2.9697 mL	7.4243 mL
	15 mM	0.1980 mL	0.9899 mL	1.9798 mL	4.9496 mL
	20 mM	0.1485 mL	0.7424 mL	1.4849 mL	3.7122 mL
	25 mM	0.1188 mL	0.5939 mL	1.1879 mL	2.9697 mL
	30 mM	0.0990 mL	0.4950 mL	0.9899 mL	2.4748 mL
	40 mM	0.0742 mL	0.3712 mL	0.7424 mL	1.8561 mL
	50 mM	0.0594 mL	0.2970 mL	0.5939 mL	1.4849 mL
	60 mM	0.0495 mL	0.2475 mL	0.4950 mL	1.2374 mL
	80 mM	0.0371 mL	0.1856 mL	0.3712 mL	0.9280 mL
	100 mM	0.0297 mL	0.1485 mL	0.2970 mL	0.7424 mL

4-Nitrophenyl 4-guanidinobenzoate hydrochloride Related Classifications

Endocrinology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

4-Nitrophenyl 4-guanidinobenzoate19135-17-2NPGBSer/Thr ProteaseSerine proteasesSerine endopeptidasesThreonine proteasesInhibitorinhibitorinhibit

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