2. Others
  3. Biochemical Assay Reagents
  4. 4-Nitrophenyl phosphate disodium hexahydrate

4-Nitrophenyl phosphate disodium hexahydrate  (Synonyms: p-Nitrophenyl phosphate disodium hexahydrate)

Cat. No.: HY-116022A Purity: 99.80%
SDS COA Handling Instructions

Molarity Calculator

4-Nitrophenyl phosphate (p-nitrophenyl phosphate) disodium hexahydrate is widely used as a small molecule phosphotyrosine-like substrate in activity assays for protein tyrosine phosphatases. 4-Nitrophenyl phosphate disodium hexahydrate is a colorless substrate that upon hydrolysis is converted to a yellow 4-nitrophenolate ion that can be monitored by absorbance at 405 nm.

For research use only. We do not sell to patients.

4-Nitrophenyl phosphate disodium hexahydrate Chemical Structure

4-Nitrophenyl phosphate disodium hexahydrate Chemical Structure

CAS No. : 333338-18-4

Size Price Stock Quantity
Free Sample (0.1 - 0.5 mg)   Apply Now  
1 g USD 50 In-stock
5 g   Get quote  
10 g   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 3 publication(s) in Google Scholar

Other Forms of 4-Nitrophenyl phosphate disodium hexahydrate:

4-Nitrophenyl phosphate disodium hexahydrate Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

4-Nitrophenyl phosphate (p-nitrophenyl phosphate) disodium hexahydrate is widely used as a small molecule phosphotyrosine-like substrate in activity assays for protein tyrosine phosphatases. 4-Nitrophenyl phosphate disodium hexahydrate is a colorless substrate that upon hydrolysis is converted to a yellow 4-nitrophenolate ion that can be monitored by absorbance at 405 nm[1].

In Vitro

4-Nitrophenyl phosphate (PNPP) disodium hexahydrate is a commonly used substrate for alkaline phosphatases (ALPs). 4-Nitrophenyl phosphate disodium hexahydrate is hydrolyzed by ALP to PNP (p-nitrophenol), which quenches the fluorescence of novel gold nanoclusters (AuNCs) by the inner filter effect (IFE)[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

4-Nitrophenyl phosphate disodium hexahydrate Related Antibodies
Molecular Weight

371.14

Formula

C6H6NOP.6H2O.2Na
CAS No.
Appearance

Solid

Color

Off-white to light yellow

SMILES

O=[N+]([O-])C1=CC=C(C=C1)OP(O[Na])(O[Na])=O.[6H2O]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : 100 mg/mL (269.44 mM; Need ultrasonic)

DMSO : 10 mg/mL (26.94 mM; ultrasonic and warming and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6944 mL 13.4720 mL 26.9440 mL
5 mM 0.5389 mL 2.6944 mL 5.3888 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.80%

SDS (394 KB)

COA (252 KB) HNMR (252 KB) RP-HPLC (91 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 2.6944 mL 13.4720 mL 26.9440 mL 67.3600 mL
5 mM 0.5389 mL 2.6944 mL 5.3888 mL 13.4720 mL
10 mM 0.2694 mL 1.3472 mL 2.6944 mL 6.7360 mL
15 mM 0.1796 mL 0.8981 mL 1.7963 mL 4.4907 mL
20 mM 0.1347 mL 0.6736 mL 1.3472 mL 3.3680 mL
25 mM 0.1078 mL 0.5389 mL 1.0778 mL 2.6944 mL
H2O 30 mM 0.0898 mL 0.4491 mL 0.8981 mL 2.2453 mL
40 mM 0.0674 mL 0.3368 mL 0.6736 mL 1.6840 mL
50 mM 0.0539 mL 0.2694 mL 0.5389 mL 1.3472 mL
60 mM 0.0449 mL 0.2245 mL 0.4491 mL 1.1227 mL
80 mM 0.0337 mL 0.1684 mL 0.3368 mL 0.8420 mL
100 mM 0.0269 mL 0.1347 mL 0.2694 mL 0.6736 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

4-Nitrophenyl phosphate disodium hexahydrate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

4-Nitrophenyl phosphate disodium hexahydrate333338-18-4p-Nitrophenyl phosphate disodium hexahydrateBiochemical Assay Reagentssubstrateprotein tyrosine phosphatases405 nmalkaline phosphataseALPInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
4-Nitrophenyl phosphate disodium hexahydrate
Cat. No.:
HY-116022A
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

English - EN (394 KB) Français - FR (394 KB) Deutsch - DE (394 KB) Norwegian - NO (394 KB) Español - ES (394 KB) Swedish - SV (394 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.