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  4. 4-Nitrophenyl phosphate disodium hexahydrate

4-Nitrophenyl phosphate disodium hexahydrate  (Synonyms: p-Nitrophenyl phosphate disodium hexahydrate)

Cat. No.: HY-116022A Purity: 99.80%
COA Handling Instructions

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4-Nitrophenyl phosphate (p-nitrophenyl phosphate) disodium hexahydrate is widely used as a small molecule phosphotyrosine-like substrate in activity assays for protein tyrosine phosphatases. 4-Nitrophenyl phosphate disodium hexahydrate is a colorless substrate that upon hydrolysis is converted to a yellow 4-nitrophenolate ion that can be monitored by absorbance at 405 nm.

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4-Nitrophenyl phosphate disodium hexahydrate Chemical Structure

4-Nitrophenyl phosphate disodium hexahydrate Chemical Structure

CAS No. : 333338-18-4

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Based on 3 publication(s) in Google Scholar

Other Forms of 4-Nitrophenyl phosphate disodium hexahydrate:

4-Nitrophenyl phosphate disodium hexahydrate Related Antibodies

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Description

4-Nitrophenyl phosphate (p-nitrophenyl phosphate) disodium hexahydrate is widely used as a small molecule phosphotyrosine-like substrate in activity assays for protein tyrosine phosphatases. 4-Nitrophenyl phosphate disodium hexahydrate is a colorless substrate that upon hydrolysis is converted to a yellow 4-nitrophenolate ion that can be monitored by absorbance at 405 nm[1].

In Vitro

4-Nitrophenyl phosphate (PNPP) disodium hexahydrate is a commonly used substrate for alkaline phosphatases (ALPs). 4-Nitrophenyl phosphate disodium hexahydrate is hydrolyzed by ALP to PNP (p-nitrophenol), which quenches the fluorescence of novel gold nanoclusters (AuNCs) by the inner filter effect (IFE)[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

4-Nitrophenyl phosphate disodium hexahydrate Related Antibodies
Molecular Weight

371.14

Formula

C6H6NOP.6H2O.2Na
CAS No.
Appearance

Solid

Color

Off-white to light yellow

SMILES

O=[N+]([O-])C1=CC=C(C=C1)OP(O[Na])(O[Na])=O.[6H2O]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : 100 mg/mL (269.44 mM; Need ultrasonic)

DMSO : 10 mg/mL (26.94 mM; ultrasonic and warming and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6944 mL 13.4720 mL 26.9440 mL
5 mM 0.5389 mL 2.6944 mL 5.3888 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

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Volume (start)

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Concentration (final)

C2

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V2

In Vivo Dissolution Calculator
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Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.80%

COA (252 KB) HNMR (252 KB) RP-HPLC (91 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 2.6944 mL 13.4720 mL 26.9440 mL 67.3600 mL
5 mM 0.5389 mL 2.6944 mL 5.3888 mL 13.4720 mL
10 mM 0.2694 mL 1.3472 mL 2.6944 mL 6.7360 mL
15 mM 0.1796 mL 0.8981 mL 1.7963 mL 4.4907 mL
20 mM 0.1347 mL 0.6736 mL 1.3472 mL 3.3680 mL
25 mM 0.1078 mL 0.5389 mL 1.0778 mL 2.6944 mL
H2O 30 mM 0.0898 mL 0.4491 mL 0.8981 mL 2.2453 mL
40 mM 0.0674 mL 0.3368 mL 0.6736 mL 1.6840 mL
50 mM 0.0539 mL 0.2694 mL 0.5389 mL 1.3472 mL
60 mM 0.0449 mL 0.2245 mL 0.4491 mL 1.1227 mL
80 mM 0.0337 mL 0.1684 mL 0.3368 mL 0.8420 mL
100 mM 0.0269 mL 0.1347 mL 0.2694 mL 0.6736 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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4-Nitrophenyl phosphate disodium hexahydrate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

4-Nitrophenyl phosphate disodium hexahydrate333338-18-4p-Nitrophenyl phosphate disodium hexahydrateBiochemical Assay Reagentssubstrateprotein tyrosine phosphatases405 nmalkaline phosphataseALPInhibitorinhibitorinhibit

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