5,15-Diacetyl-3-benzoyllathyrol (Synonyms: Euphorbia factor L3)

Name: 5,15-Diacetyl-3-benzoyllathyrol
Brand: MedChemExpress
SKU: HY-N0562
Rating: 4.5 (1 reviews)

Cat. No.: HY-N0562 Purity: 98.85%: FAQs
Data Sheet SDS COA Handling Instructions

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5,15-Diacetyl-3-benzoyllathyrol is one of the lathyrane diterpenoids, that has anti-cancer activity.

For research use only. We do not sell to patients.

5,15-Diacetyl-3-benzoyllathyrol Chemical Structure

CAS No. : 218916-52-0

Size	Price	Stock	Quantity
1 mg	USD 30	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 70	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 110	In-stock	Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Protocol
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References
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Description

5,15-Diacetyl-3-benzoyllathyrol is one of the lathyrane diterpenoids, that has anti-cancer activity.

Cellular Effect

Cell Line	Type	Value	Description	References
4T1	IC₅₀	> 10 μM Compound: 1	Cytotoxicity against mouse 4T1 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Cytotoxicity against mouse 4T1 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 34018743]
RAW264.7	IC₅₀	> 50 μM Compound: 7	Cytotoxicity against mouse RAW264.7 cells assessed as reduction in cell viability after 24 hrs by CCK8 assay Cytotoxicity against mouse RAW264.7 cells assessed as reduction in cell viability after 24 hrs by CCK8 assay	[PMID: 30817151]
RAW264.7	IC₅₀	8.06 μM Compound: Euphorbia factor L3	Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production preincubated for 3 hrs followed by LPS stimulation and measured after 24 hrs by Griess reagent based assay Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production preincubated for 3 hrs followed by LPS stimulation and measured after 24 hrs by Griess reagent based assay	[PMID: 35063896]
RAW264.7	IC₅₀	8.06 μM Compound: Euphorbia factor L3	Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production incubated for 3 hrs followed by LPS addition and measured after 24 hrs Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production incubated for 3 hrs followed by LPS addition and measured after 24 hrs	[PMID: 37002536]
RAW264.7	IC₅₀	8.3 μM Compound: 7	Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production preincubated for 3 hrs followed by LPS-stimulation and measured after 24 hrs by Griess assay Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production preincubated for 3 hrs followed by LPS-stimulation and measured after 24 hrs by Griess assay	[PMID: 30817151]

In Vitro

5,15-Diacetyl-3-benzoyllathyrol shows anticancer activity in vitro against lung cancer A549 cells and the IC₅₀ values are 34.04±3.99 μM. Furthermore, 5,15-Diacetyl-3-benzoyllathyrol can induce apoptosis in A549 cells via the mitochondrial pathway including loss of mitochondrial potential and release of cytochrome c^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

522.63

Formula

C₃₁H₃₈O₇

CAS No.

218916-52-0

Appearance

Solid

Color

White to off-white

SMILES

CC(O[C@@]1(C(/C(C)=C\[C@@H](C2(C)C)[C@@H]2CCC3=C)=O)[C@]([C@H]3OC(C)=O)([H])[C@@H](OC(C4=CC=CC=C4)=O)[C@@H](C)C1)=O

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (191.34 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.9134 mL	9.5670 mL	19.1340 mL
5 mM	0.3827 mL	1.9134 mL	3.8268 mL
10 mM	0.1913 mL	0.9567 mL	1.9134 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (4.78 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.85%

Select Batch:

Data Sheet (281 KB) SDS (252 KB)

COA (260 KB) HNMR (226 KB) CNMR (236 KB) RP-HPLC (314 KB) MS (182 KB)

Handling Instructions (2659 KB)

References

[1]. Zhang JY, et al. Structure identification of Euphorbia factor L3 and its induction of apoptosis through the mitochondrial pathway. Molecules. 2011 Apr 15;16(4):3222-31. [Content Brief]

Cell Assay
^[1]

Cells are harvested during logarithmic growth phase and seeded in 96-well plates at a density of 1.5×10⁴ cells/mL of final volume 190 μL/well. After incubation of 24 h, 10 μL EFL3 solution of full range concentrations is added to 96-well plates. After 68 h treatment, 10 μL MTT of 10 mM is added to each well for 4 h of maintaining at 37°C. Then, the supernatant is removed and the crystals are dissolved with 100 μL anhydrous DMSO each well. Subsequently, cell viability is measured by Model 550 Microplate reader at 540 nm and 655 nm as reference filter. Experiments are carried out at least thrice. The 50% inhibitory concentration (IC₅₀) is defined as the anticancer agent concentration causing 50% reduction in cell viability and calculated from the cytotoxicity curves. Cell survival is calculated with the following formula: survival (%)=(mean experimental absorbance/mean control absorbance) × 100%.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Zhang JY, et al. Structure identification of Euphorbia factor L3 and its induction of apoptosis through the mitochondrial pathway. Molecules. 2011 Apr 15;16(4):3222-31. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.9134 mL	9.5670 mL	19.1340 mL	47.8350 mL
	5 mM	0.3827 mL	1.9134 mL	3.8268 mL	9.5670 mL
	10 mM	0.1913 mL	0.9567 mL	1.9134 mL	4.7835 mL
	15 mM	0.1276 mL	0.6378 mL	1.2756 mL	3.1890 mL
	20 mM	0.0957 mL	0.4783 mL	0.9567 mL	2.3917 mL
	25 mM	0.0765 mL	0.3827 mL	0.7654 mL	1.9134 mL
	30 mM	0.0638 mL	0.3189 mL	0.6378 mL	1.5945 mL
	40 mM	0.0478 mL	0.2392 mL	0.4783 mL	1.1959 mL
	50 mM	0.0383 mL	0.1913 mL	0.3827 mL	0.9567 mL
	60 mM	0.0319 mL	0.1594 mL	0.3189 mL	0.7972 mL
	80 mM	0.0239 mL	0.1196 mL	0.2392 mL	0.5979 mL
	100 mM	0.0191 mL	0.0957 mL	0.1913 mL	0.4783 mL

5,15-Diacetyl-3-benzoyllathyrol Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

5,15-Diacetyl-3-benzoyllathyrol218916-52-0Euphorbia factor L3Euphorbia factor L 3Euphorbia factor L-3OthersInhibitorinhibitorinhibit

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5,15-Diacetyl-3-benzoyllathyrol (Synonyms: Euphorbia factor L3)

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