5,6-Dihydrouridine

Name: 5,6-Dihydrouridine
Brand: MedChemExpress
SKU: HY-113047
Rating: 5.0 (2 reviews)

Cat. No.: HY-113047 Purity: 99.90%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

5,6-Dihydrouridine is a modified base found in conserved positions in the D-loop of tRNA in Bacteria, Eukaryota, and some Archaea.

For research use only. We do not sell to patients.

5,6-Dihydrouridine Chemical Structure

CAS No. : 5627-05-4

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 110	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 110	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 100	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 150	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 240	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 360	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 540	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 2 publication(s) in Google Scholar

2 Publications Citing Use of MCE 5,6-Dihydrouridine

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Purity & Documentation
References
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Description

5,6-Dihydrouridine is a modified base found in conserved positions in the D-loop of tRNA in Bacteria, Eukaryota, and some Archaea.

IC₅₀ & Target

Human Endogenous Metabolite

In Vitro

5,6-Dihydrouridine (Dihydrouridine) is one of the posttranscriptionally modified nucleosides. It is a product of the reduction of uridine (U), and can be further modified to 5-methyldihydrouridine (m5D). 5,6-Dihydrouridine is commonly present in the tRNA from Bacteria, Eukaryota, and some Archaea. It is identified in six positions in the “D-loop” of the tRNA (16, 17, 20a, 20b) and in position 47 in the variable loop^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

246.22

Formula

C₉H₁₄N₂O₆

CAS No.

5627-05-4

Appearance

Solid

Color

White to off-white

SMILES

O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N(C(N2)=O)CCC2=O

Structure Classification

Others

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (406.14 mM; Need ultrasonic)

DMSO : 35.71 mg/mL (145.03 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.0614 mL	20.3070 mL	40.6141 mL
5 mM	0.8123 mL	4.0614 mL	8.1228 mL
10 mM	0.4061 mL	2.0307 mL	4.0614 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (8.45 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (8.45 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (8.45 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 65 mg/mL (263.99 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.90%

Select Batch:

Data Sheet (276 KB) SDS (251 KB)

COA (252 KB) LCMS (259 KB)

Handling Instructions (2659 KB)

References

[1]. Kasprzak JM, et al. Molecular evolution of dihydrouridine synthases. BMC Bioinformatics. 2012 Jun 28;13:153. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	4.0614 mL	20.3070 mL	40.6141 mL	101.5352 mL
	5 mM	0.8123 mL	4.0614 mL	8.1228 mL	20.3070 mL
	10 mM	0.4061 mL	2.0307 mL	4.0614 mL	10.1535 mL
	15 mM	0.2708 mL	1.3538 mL	2.7076 mL	6.7690 mL
	20 mM	0.2031 mL	1.0154 mL	2.0307 mL	5.0768 mL
	25 mM	0.1625 mL	0.8123 mL	1.6246 mL	4.0614 mL
	30 mM	0.1354 mL	0.6769 mL	1.3538 mL	3.3845 mL
	40 mM	0.1015 mL	0.5077 mL	1.0154 mL	2.5384 mL
	50 mM	0.0812 mL	0.4061 mL	0.8123 mL	2.0307 mL
	60 mM	0.0677 mL	0.3385 mL	0.6769 mL	1.6923 mL
	80 mM	0.0508 mL	0.2538 mL	0.5077 mL	1.2692 mL
	100 mM	0.0406 mL	0.2031 mL	0.4061 mL	1.0154 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.