5-Nitro-1,10-phenanthroline (Synonyms: 5-NP)

Cat. No.: HY-W004570 Purity: 99.88%: FAQs
Data Sheet SDS COA Handling Instructions

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5-Nitro-1,10-phenanthroline (5-NP), is a o-Phenanthroline (HY-W004544) derivative, as a mediator of glucose oxidase (GOX) with antituberculous activity. 5-Nitro-1,10-phenanthroline can be applied as redox mediators for oxidases and is suitable for the development of reagent-less biosensors and biofuel cells.

For research use only. We do not sell to patients.

5-Nitro-1,10-phenanthroline Chemical Structure

CAS No. : 4199-88-6

Size	Price	Stock	Quantity
100 mg	USD 30	In-stock	Estimated Time of Arrival: December 31
500 mg	USD 112	Get quote	Estimated Time of Arrival: December 31
1 g	USD 180	Get quote	Estimated Time of Arrival: December 31
5 g	USD 612	Get quote	Estimated Time of Arrival: December 31
10 g		Get quote
50 g		Get quote

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Purity & Documentation
References
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Description

In Vitro

5-Nitro-1,10-phenanthroline (25 μM; 24 h) kills naturally resistant intracellular bacteria by inducing autophagy in THP-1 macrophages^[2].
5-Nitro-1,10-phenanthroline (1x, 5x, 20x or 50x MIC, MIC=0.78 μM; 1 h) also modulates the host machinery to kill intracellular pathogens by inhibiting mycolic acid biosynthesis of Mtb^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:	Mtb H37Rv, M. bovis BCG and M. bovis BCG-5NP resistant strain
Concentration:	0-12.5 μM
Incubation Time:	24 hours
Result:	Inhibited pathogens with MIC₉₉ values of 0.78 μM (Mtb H37Rv), 0.78 μM (M. bovis BCG), and >12.5 μM (M. bovis BCG-5NP), respectively.

Molecular Weight

225.20

Formula

C₁₂H₇N₃O₂

CAS No.

4199-88-6

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=[N+]([O-])C1=C2C(N=CC=C2)=C3C(C=CC=N3)=C1

Structure Classification

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility

In Vitro:

Ethanol : 10 mg/mL (44.40 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.4405 mL	22.2025 mL	44.4050 mL
5 mM	0.8881 mL	4.4405 mL	8.8810 mL
10 mM	0.4440 mL	2.2202 mL	4.4405 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.88%

Select Batch:

Data Sheet (279 KB) SDS (393 KB)

COA (255 KB) HNMR (117 KB) RP-HPLC (192 KB)

Handling Instructions (2659 KB)

References

[1]. Oztekin Y, et al. 1,10-Phenanthroline derivatives as mediators for glucose oxidase. Biosens Bioelectron. 2010 Sep 15;26(1):267-70. [Content Brief]

[2]. Kidwai S, et al. Dual Mechanism of Action of 5-Nitro-1,10-Phenanthroline against Mycobacterium tuberculosis. Antimicrob Agents Chemother. 2017 Oct 24;61(11):e00969-17. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

Ethanol	1 mM	4.4405 mL	22.2025 mL	44.4050 mL	111.0124 mL
	5 mM	0.8881 mL	4.4405 mL	8.8810 mL	22.2025 mL
	10 mM	0.4440 mL	2.2202 mL	4.4405 mL	11.1012 mL
	15 mM	0.2960 mL	1.4802 mL	2.9603 mL	7.4008 mL
	20 mM	0.2220 mL	1.1101 mL	2.2202 mL	5.5506 mL
	25 mM	0.1776 mL	0.8881 mL	1.7762 mL	4.4405 mL
	30 mM	0.1480 mL	0.7401 mL	1.4802 mL	3.7004 mL
	40 mM	0.1110 mL	0.5551 mL	1.1101 mL	2.7753 mL

5-Nitro-1,10-phenanthroline Related Classifications

Infection

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

5-Nitro-1,10-phenanthroline4199-88-65-NPEndogenous Metabolitereagent-less biosensorsbiofuel cellsredox mediatorso-PhenanthrolineF420 dependenceMycobacterium tuberculosisautophagyphenotypic screeningInhibitorinhibitorinhibit

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