2. Immunology/Inflammation Metabolic Enzyme/Protease
  3. NO Synthase Endogenous Metabolite
  4. 6-Biopterin

6-Biopterin  (Synonyms: L-Biopterin)

Cat. No.: HY-102015 Purity: 99.04%
COA Handling Instructions

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6-Biopterin (L-Biopterin), a pterin derivative, is a NO synthase cofactor.

For research use only. We do not sell to patients.

6-Biopterin Chemical Structure

6-Biopterin Chemical Structure

CAS No. : 22150-76-1

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 40 In-stock
Solution
10 mM * 1 mL in DMSO USD 40 In-stock
Solid
5 mg USD 37 In-stock
10 mg USD 60 In-stock
25 mg USD 130 In-stock
50 mg USD 210 In-stock
100 mg USD 340 In-stock
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

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Based on 1 publication(s) in Google Scholar

6-Biopterin Related Antibodies

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nNOS iNOS eNOS

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

6-Biopterin (L-Biopterin), a pterin derivative, is a NO synthase cofactor.

IC50 & Target

Human Endogenous Metabolite

 

In Vitro

6-biopterin is extremely cytotoxic to human melanocytes under in vitro conditions. Thioredoxin reductase has the capacity to reduce 6-biopterin to q-BH2 where further reduction to 6-BH4 follows via dihydropteridine reductase or reduced glutathione. (6R)5,6,7,8 tetrahydrobiopterin undergoes redox-cycling by its oxidation to quinonoid dihydrobiopterin and to 6-biopterin through consecutive two electron oxidation reactions[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

6-Biopterin Related Antibodies
Molecular Weight

237.22

Formula

C9H11N5O3
CAS No.
Appearance

Solid

Color

White to light yellow

SMILES

O=C1N=C(N)NC2=NC=C([C@@H](O)[C@@H](O)C)N=C12
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 12.5 mg/mL (52.69 mM; ultrasonic and warming and heat to 75°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.2155 mL 21.0775 mL 42.1550 mL
5 mM 0.8431 mL 4.2155 mL 8.4310 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: 1 mg/mL (4.22 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 1 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 1 mg/mL (4.22 mM); Suspended solution

    This protocol yields a suspended solution of ≥ 1 mg/mL (saturation unknown). Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.04%

COA (248 KB) HNMR (274 KB) RP-HPLC (249 KB) MS (68 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.2155 mL 21.0775 mL 42.1550 mL 105.3874 mL
5 mM 0.8431 mL 4.2155 mL 8.4310 mL 21.0775 mL
10 mM 0.4215 mL 2.1077 mL 4.2155 mL 10.5387 mL
15 mM 0.2810 mL 1.4052 mL 2.8103 mL 7.0258 mL
20 mM 0.2108 mL 1.0539 mL 2.1077 mL 5.2694 mL
25 mM 0.1686 mL 0.8431 mL 1.6862 mL 4.2155 mL
30 mM 0.1405 mL 0.7026 mL 1.4052 mL 3.5129 mL
40 mM 0.1054 mL 0.5269 mL 1.0539 mL 2.6347 mL
50 mM 0.0843 mL 0.4215 mL 0.8431 mL 2.1077 mL
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6-Biopterin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

6-Biopterin22150-76-1L-BiopterinNO SynthaseEndogenous MetaboliteNitric oxide synthasesNOSInhibitorinhibitorinhibit

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