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  4. 7-Deaza-7-propargylamino-3'-azidomethyl-dATP triethylamine

7-Deaza-7-propargylamino-3'-azidomethyl-dATP triethylamine 

Cat. No.: HY-132139A
Data Sheet Handling Instructions Technical Support

Solubility

In Vivo Dissolution Calculator

Help & FAQs

7-Deaza-7-propargylamino-3'-azidomethyl-dATP triethylamine is an analog of deoxy adenosine triphosphate (dATP) that widely used in the next generation sequencing (NGS).

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7-Deaza-7-propargylamino-3'-azidomethyl-dATP triethylamine Chemical Structure

7-Deaza-7-propargylamino-3'-azidomethyl-dATP triethylamine Chemical Structure

Size Price Stock Quantity
1 mg USD 350 In-stock
5 mg USD 890 In-stock
10 mg   Get quote  
50 mg   Get quote  

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Other Forms of 7-Deaza-7-propargylamino-3'-azidomethyl-dATP triethylamine:

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  • Purity & Documentation

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Description

7-Deaza-7-propargylamino-3'-azidomethyl-dATP triethylamine is an analog of deoxy adenosine triphosphate (dATP) that widely used in the next generation sequencing (NGS)[1].

Molecular Weight

982.81

Formula

C15H21N8O12P3.3.8C6H15N
Appearance

Solid

SMILES

NCC#CC1=CN([C@@H](C[C@@H]2OCN=[N+]=[N-])O[C@@H]2COP(OP(OP(O)(O)=O)(O)=O)(O)=O)C3=NC=NC(N)=C31.CCN(CC)CC.[3.8]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : 125 mg/mL (127.19 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.0175 mL 5.0875 mL 10.1749 mL
5 mM 0.2035 mL 1.0175 mL 2.0350 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 1.0175 mL 5.0875 mL 10.1749 mL 25.4373 mL
5 mM 0.2035 mL 1.0175 mL 2.0350 mL 5.0875 mL
10 mM 0.1017 mL 0.5087 mL 1.0175 mL 2.5437 mL
15 mM 0.0678 mL 0.3392 mL 0.6783 mL 1.6958 mL
20 mM 0.0509 mL 0.2544 mL 0.5087 mL 1.2719 mL
25 mM 0.0407 mL 0.2035 mL 0.4070 mL 1.0175 mL
30 mM 0.0339 mL 0.1696 mL 0.3392 mL 0.8479 mL
40 mM 0.0254 mL 0.1272 mL 0.2544 mL 0.6359 mL
50 mM 0.0203 mL 0.1017 mL 0.2035 mL 0.5087 mL
60 mM 0.0170 mL 0.0848 mL 0.1696 mL 0.4240 mL
80 mM 0.0127 mL 0.0636 mL 0.1272 mL 0.3180 mL
100 mM 0.0102 mL 0.0509 mL 0.1017 mL 0.2544 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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7-Deaza-7-propargylamino-3'-azidomethyl-dATP triethylamine Related Classifications

Help & FAQs
  • How should lyophilized recombinant proteins be reconstituted and stored?

    1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss. 2. Taking 10 μg as an example, first add 20 μL of reconstituted solution provided by MCE and use a pipette to gently resuspend the lyophilized protein until it is fully dissolved.. (For most proteins, the reconstitution solution we provide is sterile water. If a diluent other than water is required, it will be indicated in the product's Certificate of Analysis (COA).). 3. Add an additional 80 μL of buffer/culture medium containing carrier protein (either 0.1% BSA, 5% HSA, 10% FBS, or 5% trehalose), and then use a pipette to gently mix until uniform. The final concentration is should not be lower than 100 μg/mL. 4. Aliquot at least 20 μL per tube. 5. After aliquoting, store it frozen at a temperature ranging from -20ºC to -80ºC, and it can be preserved for 3 to 6 months.

  • How should solution-form recombinant proteins be stored?

    1. The product can be stored in its original form and diluted as needed upon use. 2. Alternatively, dilute with a buffer/culture medium containing a carrier protein (either 0.1% BSA, 5% HSA, 10% FBS, or 5% alginate), mix well by pipetting, and ensure that

  • Why is it necessary to add carrier proteins?

    Carrier proteins are commonly added to enhance the stability of recombinant proteins, preventing them from adhering to the walls of the container during freezing or thawing processes. Plastic tubes have a certain adsorptive capacity for proteins, which may lead to difficulty in separating the protein from the tube walls, resulting in a decrease in the actual concentration of the protein in the solution and thus affecting its activity. To minimize such losses, it is recommended to add a commonly used carrier protein solution prior to the long-term storage of recombinant protein products.

  • Carrier protein types and options?

    In cases where the carrier protein is not expected to influence the experimental outcomes, an appropriate carrier protein, such as 0.1% BSA (Bovine Serum Albumin), 5% HSA (Human Serum Albumin), 10% FBS (Fetal Bovine Serum), or 5% trehalose, can be incorpo

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