8-CPT-2Me-cAMP sodium

Name: 8-CPT-2Me-cAMP sodium
Brand: MedChemExpress
SKU: HY-107543
Rating: 4.5 (1 reviews)

Cat. No.: HY-107543 Purity: 99.58%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

8-CPT-2Me-cAMP sodium is a selective activator of exchange proteins activated by cAMP (Epac), the cAMP sensitive guanine nucleotide exchange factors (GEFs) for the small GTPases Rap1 and Rap2. 8-CPT-2Me-cAMP sodium activates Epac1 (EC₅₀ = 2.2 μM), but not PKA (EC₅₀> 10 μM). 8-CPT-2Me-cAMP sodium stimulates Epac-mediated Ca²⁺ release in pancreatic β-cells in vitro.

For research use only. We do not sell to patients.

8-CPT-2Me-cAMP sodium Chemical Structure

CAS No. : 634207-53-7

Size	Price	Stock	Quantity
1 mg	USD 170	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 540	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 860	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 1380	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 2200	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of 8-CPT-2Me-cAMP sodium:

8-CPT-2'-O-Me-cAMP Get quote

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Purity & Documentation
References
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Description

8-CPT-2Me-cAMP sodium is a selective activator of exchange proteins activated by cAMP (Epac), the cAMP sensitive guanine nucleotide exchange factors (GEFs) for the small GTPases Rap1 and Rap2. 8-CPT-2Me-cAMP sodium activates Epac1 (EC₅₀ = 2.2 μM), but not PKA (EC₅₀> 10 μM)^[1]. 8-CPT-2Me-cAMP sodium stimulates Epac-mediated Ca²⁺ release in pancreatic β-cells in vitro^[2].

Molecular Weight

507.82

Formula

C₁₇H₁₆ClN₅NaO₆PS

CAS No.

634207-53-7

Appearance

Solid

Color

White to off-white

SMILES

NC1=C2C(N([C@H]3[C@@H]([C@@](O4)([H])[C@](COP4(O[Na])=O)([H])O3)OC)C(SC5=CC=C(Cl)C=C5)=N2)=NC=N1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (246.15 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.9692 mL	9.8460 mL	19.6920 mL
5 mM	0.3938 mL	1.9692 mL	3.9384 mL
10 mM	0.1969 mL	0.9846 mL	1.9692 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.10 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (4.10 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.58%

Select Batch:

Data Sheet (278 KB) SDS (252 KB)

COA (255 KB) HNMR (320 KB) LCMS (266 KB)

Handling Instructions (2659 KB)

References

[1]. Enserink JM, Christensen AE, de Rooij J, van Triest M, Schwede F, Genieser HG, Døskeland SO, Blank JL, Bos JL. A novel Epac-specific cAMP analogue demonstrates independent regulation of Rap1 and ERK. Nat Cell Biol. 2002 Nov;4(11):901-6. [Content Brief]

[2]. Kang G, Joseph JW, Chepurny OG, Monaco M, Wheeler MB, Bos JL, Schwede F, Genieser HG, Holz GG. Epac-selective cAMP analog 8-pCPT-2'-O-Me-cAMP as a stimulus for Ca2+-induced Ca2+ release and exocytosis in pancreatic beta-cells. J Biol Chem. 2003 Mar 7;278( [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.9692 mL	9.8460 mL	19.6920 mL	49.2300 mL
	5 mM	0.3938 mL	1.9692 mL	3.9384 mL	9.8460 mL
	10 mM	0.1969 mL	0.9846 mL	1.9692 mL	4.9230 mL
	15 mM	0.1313 mL	0.6564 mL	1.3128 mL	3.2820 mL
	20 mM	0.0985 mL	0.4923 mL	0.9846 mL	2.4615 mL
	25 mM	0.0788 mL	0.3938 mL	0.7877 mL	1.9692 mL
	30 mM	0.0656 mL	0.3282 mL	0.6564 mL	1.6410 mL
	40 mM	0.0492 mL	0.2462 mL	0.4923 mL	1.2308 mL
	50 mM	0.0394 mL	0.1969 mL	0.3938 mL	0.9846 mL
	60 mM	0.0328 mL	0.1641 mL	0.3282 mL	0.8205 mL
	80 mM	0.0246 mL	0.1231 mL	0.2462 mL	0.6154 mL
	100 mM	0.0197 mL	0.0985 mL	0.1969 mL	0.4923 mL