2. Metabolic Enzyme/Protease
  3. Phosphatase
  4. BAMB-4

BAMB-4  (Synonyms: ITPKA-IN-C14)

Cat. No.: HY-16694 Purity: 99.99%
COA Handling Instructions

Molarity Calculator

BAMB-4 (ITPKA-IN-C14) is a specific and membrane-permeable ITPKA inhibitor. BAMB-4 has high stability and membrane permeability and against the inositol-1,4,5-trisphosphate (InsP3) kinase activity of inositol-1,4,5-trisphosphate-3-kinase A (ITPKA) with an IC50 value of 20 μM. BAMB-4 can be used for the research of metastasis of lung cancer.

For research use only. We do not sell to patients.

BAMB-4 Chemical Structure

BAMB-4 Chemical Structure

CAS No. : 891025-25-5

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 257 In-stock
Solution
10 mM * 1 mL in DMSO USD 257 In-stock
Solid
2 mg USD 160 In-stock
5 mg USD 250 In-stock
10 mg USD 375 In-stock
25 mg USD 675 In-stock
50 mg USD 945 In-stock
100 mg USD 1323 In-stock
200 mg   Get quote  
500 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

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Based on 1 publication(s) in Google Scholar

BAMB-4 Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

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Description

BAMB-4 (ITPKA-IN-C14) is a specific and membrane-permeable ITPKA inhibitor. BAMB-4 has high stability and membrane permeability and against the inositol-1,4,5-trisphosphate (InsP3) kinase activity of inositol-1,4,5-trisphosphate-3-kinase A (ITPKA) with an IC50 value of 20 μM. BAMB-4 can be used for the research of metastasis of lung cancer[1].

IC50 & Target

IC50: 37 μM (ITPKA); 20 μM (InsP3 kinase)[1]
In Vitro

BAMB-4 (0.3-40 μM) has ITPKA inhibiton effect with an IC50 value of 37 μM[1].
BAMB-4 (40 μM) inhibits InsP3 kinase activity with an IC50 value of 20 μM[1].
BAMB-4 (100 μM, overnight) has high specificity and the high cellular uptake[1].
BAMP-4 (0, 10, 20 and 40 μM) shows increased Km and decreased V max [1].
BAMP-4 (0-30 μM) is a mixed type inhibitor with respect to ATP and InsP3 and does not only affect binding of ATP but also binding of InsP3，which affects turnover of two substrate exhibit a higher inhibition specifcity[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

BAMB-4 Related Antibodies
Molecular Weight

252.27

Formula

C15H12N2O2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(C1=CC=C(C)C=C1)NC2=NOC3=CC=CC=C23
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : ≥ 40 mg/mL (158.56 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.9640 mL 19.8200 mL 39.6401 mL
5 mM 0.7928 mL 3.9640 mL 7.9280 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (9.91 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.99%

COA (253 KB) LCMS (440 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.9640 mL 19.8200 mL 39.6401 mL 99.1002 mL
5 mM 0.7928 mL 3.9640 mL 7.9280 mL 19.8200 mL
10 mM 0.3964 mL 1.9820 mL 3.9640 mL 9.9100 mL
15 mM 0.2643 mL 1.3213 mL 2.6427 mL 6.6067 mL
20 mM 0.1982 mL 0.9910 mL 1.9820 mL 4.9550 mL
25 mM 0.1586 mL 0.7928 mL 1.5856 mL 3.9640 mL
30 mM 0.1321 mL 0.6607 mL 1.3213 mL 3.3033 mL
40 mM 0.0991 mL 0.4955 mL 0.9910 mL 2.4775 mL
50 mM 0.0793 mL 0.3964 mL 0.7928 mL 1.9820 mL
60 mM 0.0661 mL 0.3303 mL 0.6607 mL 1.6517 mL
80 mM 0.0496 mL 0.2478 mL 0.4955 mL 1.2388 mL
100 mM 0.0396 mL 0.1982 mL 0.3964 mL 0.9910 mL
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BAMB-4 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

BAMB-4891025-25-5ITPKA-IN-C14BAMB4BAMB 4Phosphatasecellular uptakemixed inhibition typeinositol-1,4,5-trisphosphate-3-kinase A (ITPKA)inositol-1,4,5-trisphosphate (InsP3)kinaselung cancerInhibitorinhibitorinhibit

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