BAM 15

Name: BAM 15
Brand: MedChemExpress
SKU: HY-110284
Rating: 5.0 (3 reviews)

Cat. No.: HY-110284 Purity: 99.92%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

BAM 15 is a mitochondrial protonophore uncoupler. BAM 15 is an oxidative phosphorylation (OXPHOS) uncoupler.

For research use only. We do not sell to patients.

BAM 15 Chemical Structure

CAS No. : 210302-17-3

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 83	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 83	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 75	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 135	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 250	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 400	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 600	In-stock	Estimated Time of Arrival: December 31
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500 mg		Get quote

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Customer Review

Based on 3 publication(s) in Google Scholar

3 Publications Citing Use of MCE BAM 15

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Biological Activity
Protocol
Purity & Documentation
References
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Description

BAM 15 is a mitochondrial protonophore uncoupler. BAM 15 is an oxidative phosphorylation (OXPHOS) uncoupler^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
L6	EC₅₀	0.31 μM Compound: 8; BAM15	Induction of mitochondrial uncoupling in rat L6 cells assessed as increase in oxygen consumption rate incubated for 90 mins by Seahorse XF analyzer based assay Induction of mitochondrial uncoupling in rat L6 cells assessed as increase in oxygen consumption rate incubated for 90 mins by Seahorse XF analyzer based assay	[PMID: 32392051]
L6	EC₅₀	0.31 μM Compound: BAM15	Induction of mitochondrial uncoupled respiration in rat L6 myoblasts assessed as increase in oxygen consumption rate measured by Agilent Seahorse XF analyzer Induction of mitochondrial uncoupled respiration in rat L6 myoblasts assessed as increase in oxygen consumption rate measured by Agilent Seahorse XF analyzer	[PMID: 35907607]
L6	EC₅₀	0.49 μM Compound: 5; BAM15	Induction of mitochondrial uncoupling in rat L6 cells assessed as oxygen consumption rate measured over 90 mins by oxygen consumption rate by seahorse assay Induction of mitochondrial uncoupling in rat L6 cells assessed as oxygen consumption rate measured over 90 mins by oxygen consumption rate by seahorse assay	[PMID: 32017849]
MCF7	EC₅₀	0.31 μM Compound: BAM15	Antiproliferative activity against human MCF7 assessed as reduction in cell viability incubated for 24 hrs by MTT assay Antiproliferative activity against human MCF7 assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 33569941]

In Vitro

BAM 15 is able to increase O₂ consumption across a broad dosing range without increasing ROS. BAM 15 and FCCP are structurally unrelated and it is observed that low doses of BAM 15 from 100 nM to 1 μM increase cellular O₂ consumption rate (OCR) to a similar degree as FCCP, but higher concentrations from 1 μM to 50 μM reveal that BAM 15 is able to maintain uncoupled respiration at a high rate in a range of cell lines. BAM 15 is fully capable of increasing mitochondrial respiration in the presence of oligomycin and does so across a broader concentration range than FCCP in both myoblasts and hepatocytes. BAM 15 induces mitochondrial swelling, demonstrating that BAM 15 is a protonophore. BAM15-treated cells are more viable than FCCP-treated cells when administered across a broad dosing range up to 50 μM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Compare to vehicle-treated mice, animals that receive BAM 15 are protected from kidney injury as indicated by lower plasma creatinine levels at 24 and 48 h post-ischemia, reduced tubular necrosis, less depletion of brush border villi, less obstruction of proximal tubules, and less immune cell infiltration^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

340.29

Formula

C₁₆H₁₀F₂N₆O

CAS No.

210302-17-3

Appearance

Solid

Color

Light yellow to yellow

SMILES

FC(C=CC=C1)=C1NC2=NC3=NON=C3N=C2NC4=C(C=CC=C4)F

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	1 year
	-20°C	6 months

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (146.93 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.9387 mL	14.6933 mL	29.3867 mL
5 mM	0.5877 mL	2.9387 mL	5.8773 mL
10 mM	0.2939 mL	1.4693 mL	2.9387 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.35 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.92%

Select Batch:

Data Sheet (279 KB) SDS (761 KB)

COA (253 KB) HNMR (279 KB) LCMS (265 KB)

Handling Instructions (2659 KB)

References

[1]. Kenwood BM, et al. Identification of a novel mitochondrial uncoupler that does not depolarize the plasma membrane. Mol Metab. 2013 Nov 28;3(2):114-23. [Content Brief]

Kinase Assay ^[1]	Electron flow assays are performed. Briefly, 5 μg of mitochondrial protein in MAS is loaded into a 24-well tissue culture plate and centrifuged at 2000×g for 15 min at 4°C. Prior to the assay, mitochondria are incubated at 37°C for 10 mins in MAS containing 10 mM pyruvate, 2 mM malate, and 5 μM BAM 15 or FCCP. Rotenone (2 μM), succinate (10 mM), antimycin A (4 μM), and N,N,N′,N′-tetramethyl-p-phenylenediamine (TMPD, 100 μM) plus ascorbate (10 mM) are added sequentially as indicated in the figure. N=3 wells/plate of a representative of 3 plates^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Assay ^[1]	L6 cells are incubated with the fluorescent indicator of mitochondrial membrane potential tetramethylrhodamine (TMRM, 125 nM) or DMSO (1%) control for 30 min. The cells are then centrifuged for 5 min at 700×g and resuspended in unbuffered DMEM at a concentration of 1×10⁵ cells/mL. The cells are then treated with BAM 15 or DMSO (0.1%) for 10 min prior to flow cytometric analysis^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration ^[1]	Male mice (8-week old, C57BL/6) are used. Mice are i.p. injected with BAM 15 at 1 or 5 mg/kg, 1 h before kidney IR. Vehicle mice are also injected with the same solution BAM 15 is prepared with (3% DMSO in 50% PEG400)^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References	[1]. Kenwood BM, et al. Identification of a novel mitochondrial uncoupler that does not depolarize the plasma membrane. Mol Metab. 2013 Nov 28;3(2):114-23. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.9387 mL	14.6933 mL	29.3867 mL	73.4667 mL
	5 mM	0.5877 mL	2.9387 mL	5.8773 mL	14.6933 mL
	10 mM	0.2939 mL	1.4693 mL	2.9387 mL	7.3467 mL
	15 mM	0.1959 mL	0.9796 mL	1.9591 mL	4.8978 mL
	20 mM	0.1469 mL	0.7347 mL	1.4693 mL	3.6733 mL
	25 mM	0.1175 mL	0.5877 mL	1.1755 mL	2.9387 mL
	30 mM	0.0980 mL	0.4898 mL	0.9796 mL	2.4489 mL
	40 mM	0.0735 mL	0.3673 mL	0.7347 mL	1.8367 mL
	50 mM	0.0588 mL	0.2939 mL	0.5877 mL	1.4693 mL
	60 mM	0.0490 mL	0.2449 mL	0.4898 mL	1.2244 mL
	80 mM	0.0367 mL	0.1837 mL	0.3673 mL	0.9183 mL
	100 mM	0.0294 mL	0.1469 mL	0.2939 mL	0.7347 mL

BAM 15 Related Classifications

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

BAM 15210302-17-3BAM15BAM-15Oxidative PhosphorylationMitochondrial MetabolismInhibitorinhibitorinhibit

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BAM 15

Customer Review

BAM 15 Related Antibodies

3 Publications Citing Use of MCE BAM 15

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•Related Screening Libraries:

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Purity & Documentation

References

Customer Review

BAM 15 Related Antibodies

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Complete Stock Solution Preparation Table

BAM 15 Related Classifications

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