Beaucage reagent

Name: Beaucage reagent
Brand: MedChemExpress
SKU: HY-100951
Rating: 4.5 (1 reviews)

Cat. No.: HY-100951 Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Beaucage reagent is found to be potent in causing DNA cleavage.

For research use only. We do not sell to patients.

Beaucage reagent Chemical Structure

CAS No. : 66304-01-6

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10 mM * 1 mL in DMSO ready for reconstitution	USD 55	In-stock	Estimated Time of Arrival: December 31
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10 mM * 1 mL in DMSO	USD 55	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 1 publication(s) in Google Scholar

1 Publications Citing Use of MCE Beaucage reagent

•J Mol Med (Berl). 2019 Aug;97(8):1183-1193. [Abstract]

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Beaucage reagent is found to be potent in causing DNA cleavage^[1]^[2]^[3].

In Vitro

The synthesis of Beaucage reagent:^[1]
Under the condition of 5℃, 2-amino-3-methyl benzoic acid is subjected to diazonium reaction following the procedure described by Allen andcoworkers[2]. Upon the diazonium salt formation, it was treated immediately with sulfide to generate disulfide under strongly alkaline conditions.Using activated zinc in refluxing glacial acetic acid, the disulfide bond was cleaved off to yield thiol 2-mercapto group-3-carboxyl benzoic acid. The reaction of thioacetic acid with 2-mercapto group-3-carboxyl benzoic acid in concentrated sulfuric acid gave rise to the cyclic compound. Employ dimethyldioxirane as potential oxidizing agent. A one-pot procedure in which Oxone^TM was added to a solution of the cyclic compound in acetone led to the successful preparation of Beaucage reagent.
Beaucage reagent causes DNA cleavage:^[1]
Assays were performed in 50 mM sodium phosphate buffer (pH 7.0) containing 0.5 mg of supercoiled pBR322 DNA in the presence of Beaucage reagent at different concentrations (total volume 20 mL) for 12 h. Beaucage reagent can effectively cause DNA-cleaving activities.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

200.23

Formula

C₇H₄O₃S₂

CAS No.

66304-01-6

Appearance

Solid

Color

White to off-white

SMILES

O=C(C1=CC=CC=C12)SS2(=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL (499.43 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 0.14 mg/mL (0.70 mM; Need ultrasonic)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.9943 mL	24.9713 mL	49.9426 mL
5 mM	0.9989 mL	4.9943 mL	9.9885 mL
10 mM	0.4994 mL	2.4971 mL	4.9943 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (12.49 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (12.49 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (321 KB) SDS (252 KB)

COA (261 KB) HNMR (192 KB)

Handling Instructions (2659 KB)

References

[1]. Zheng J, et al. Thiol-dependent DNA cleavage by aminomethylated Beaucage's reagent. Org Biomol Chem. 2010 Mar 21;8(6):1293-5. [Content Brief]

[2]. C. F. H. Allen, et al. Working with Hazardous Chemicals. Organic Syntheses, Coll. Vol. 2, 1943, 580.

[3]. Stec W J, et al. Bis (O, O-diisopropoxy phosphinothioyl) disulfide-a highly efficient sulfurizing reagent for cost-effective synthesis of oligo (nucleoside phosphorothioate) s[J]. Tetrahedron letters, 1993, 34(33): 5317-5320.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.9943 mL	24.9713 mL	49.9426 mL	124.8564 mL
	5 mM	0.9989 mL	4.9943 mL	9.9885 mL	24.9713 mL
	10 mM	0.4994 mL	2.4971 mL	4.9943 mL	12.4856 mL
	15 mM	0.3330 mL	1.6648 mL	3.3295 mL	8.3238 mL
	20 mM	0.2497 mL	1.2486 mL	2.4971 mL	6.2428 mL
	25 mM	0.1998 mL	0.9989 mL	1.9977 mL	4.9943 mL
	30 mM	0.1665 mL	0.8324 mL	1.6648 mL	4.1619 mL
	40 mM	0.1249 mL	0.6243 mL	1.2486 mL	3.1214 mL
	50 mM	0.0999 mL	0.4994 mL	0.9989 mL	2.4971 mL
	60 mM	0.0832 mL	0.4162 mL	0.8324 mL	2.0809 mL
	80 mM	0.0624 mL	0.3121 mL	0.6243 mL	1.5607 mL
	100 mM	0.0499 mL	0.2497 mL	0.4994 mL	1.2486 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.