Betulonic acid (Synonyms: Betunolic acid; Liquidambaric acid; (+)-Betulonic acid)

Name: Betulonic acid
Brand: MedChemExpress
SKU: HY-N1451
Rating: 5.0 (2 reviews)

Cat. No.: HY-N1451 Purity: 98.06%: FAQs
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Betulonic acid (Betunolic acid), a naturally occurring triterpene, is found in many plants. Betulonic acid has anti-tumor, anti-inflammatory, antiparasitic and anti-viral (HSV-1) activities.

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Betulonic acid Chemical Structure

CAS No. : 4481-62-3

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10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
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Based on 2 publication(s) in Google Scholar

Other Forms of Betulonic acid:

Betulonic acid (Standard) Get quote

2 Publications Citing Use of MCE Betulonic acid

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Biological Activity
Purity & Documentation
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Description

Betulonic acid (Betunolic acid), a naturally occurring triterpene, is found in many plants. Betulonic acid has anti-tumor, anti-inflammatory, antiparasitic and anti-viral (HSV-1) activities^[2]^[1]^[3]^[4].

IC₅₀ & Target

Plasmodium

HSV-1

Cellular Effect

Cell Line	Type	Value	Description	References
518A2	IC₅₀	10.42 μM Compound: 2	Cytotoxicity against human 518A2 cells after 96 hrs by SRB colorimetric assay Cytotoxicity against human 518A2 cells after 96 hrs by SRB colorimetric assay	[PMID: 20472329]
518A2	IC₅₀	12.93 μM Compound: 2	Cytotoxicity against human 518A2 cells assessed as cell viability after 96 hrs by SRB assay Cytotoxicity against human 518A2 cells assessed as cell viability after 96 hrs by SRB assay	[PMID: 23973824]
518A2	EC₅₀	29 μM Compound: 4	Cytotoxicity against human 518A2 cells after 96 hrs by SRB assay Cytotoxicity against human 518A2 cells after 96 hrs by SRB assay	[PMID: 26547057]
8505C	IC₅₀	14 μM Compound: 2	Cytotoxicity against human 8505C cells assessed as cell viability after 96 hrs by SRB assay Cytotoxicity against human 8505C cells assessed as cell viability after 96 hrs by SRB assay	[PMID: 23973824]
A253 cell line	IC₅₀	10.05 μM Compound: 2	Cytotoxicity against human A253 cells after 96 hrs by SRB colorimetric assay Cytotoxicity against human A253 cells after 96 hrs by SRB colorimetric assay	[PMID: 20472329]
A2780	IC₅₀	2.98 μM Compound: 2	Cytotoxicity against human A2780 cells after 96 hrs by SRB colorimetric assay Cytotoxicity against human A2780 cells after 96 hrs by SRB colorimetric assay	[PMID: 20472329]
A2780	EC₅₀	20.4 μM Compound: 4	Cytotoxicity against human A2780 cells after 96 hrs by SRB assay Cytotoxicity against human A2780 cells after 96 hrs by SRB assay	[PMID: 26547057]
A2780	EC₅₀	4.7 μM Compound: 2	Cytotoxicity against human A2780 cells assessed as reduction in cell viability incubated for 72 hrs by SRB assay Cytotoxicity against human A2780 cells assessed as reduction in cell viability incubated for 72 hrs by SRB assay	[PMID: 32956968]
A2780	IC₅₀	5.97 μM Compound: 2	Cytotoxicity against human A2780 cells assessed as cell viability after 96 hrs by SRB assay Cytotoxicity against human A2780 cells assessed as cell viability after 96 hrs by SRB assay	[PMID: 23973824]
A-375	EC₅₀	14.3 μM Compound: 2	Cytotoxicity against human A-375 cells assessed as reduction in cell viability incubated for 72 hrs by SRB assay Cytotoxicity against human A-375 cells assessed as reduction in cell viability incubated for 72 hrs by SRB assay	[PMID: 32956968]
A-375	IC₅₀	28.9 μM Compound: 1, BetA, Betulonic acid	Cytotoxicity against human A375 cells after 72 hrs by MTT assay Cytotoxicity against human A375 cells after 72 hrs by MTT assay	[PMID: 25874331]
A-431	IC₅₀	3.26 μM Compound: 2	Cytotoxicity against human A431 cells after 96 hrs by SRB colorimetric assay Cytotoxicity against human A431 cells after 96 hrs by SRB colorimetric assay	[PMID: 20472329]
A549	ED₅₀	> 10 μg/mL Compound: 13	Cytotoxic activity against human A549 cell line was measured after 72 hr using MTT assay Cytotoxic activity against human A549 cell line was measured after 72 hr using MTT assay	[PMID: 15225732]
A549	IC₅₀	10.09 μM Compound: 2	Cytotoxicity against human A549 cells after 96 hrs by SRB colorimetric assay Cytotoxicity against human A549 cells after 96 hrs by SRB colorimetric assay	[PMID: 20472329]
A549	IC₅₀	10.42 μM Compound: 2	Cytotoxicity against human A549 cells assessed as cell viability after 96 hrs by SRB assay Cytotoxicity against human A549 cells assessed as cell viability after 96 hrs by SRB assay	[PMID: 23973824]
A549	IC₅₀	15 μM Compound: 1b	Cytotoxicity against human A549 cells after 72 hrs by MTT assay Cytotoxicity against human A549 cells after 72 hrs by MTT assay	[PMID: 17371067]
A549	IC₅₀	15 μM Compound: 7, betulonic acid	Cytotoxicity against human A549 cells after 72 hrs by MTT assay Cytotoxicity against human A549 cells after 72 hrs by MTT assay	[PMID: 15270560]
A549	EC₅₀	25.9 μM Compound: 4	Cytotoxicity against human A549 cells after 96 hrs by SRB assay Cytotoxicity against human A549 cells after 96 hrs by SRB assay	[PMID: 26547057]
A549	IC₅₀	40 μM Compound: 5a	Cytotoxicity against human A549 cells incubated for 72 hrs by MTS assay Cytotoxicity against human A549 cells incubated for 72 hrs by MTS assay	[PMID: 31677446]
A549	IC₅₀	5.7 μM Compound: 7	Cytotoxicity against human A549 cells assessed as reduction in cell viability after 48 hrs by SRB assay Cytotoxicity against human A549 cells assessed as reduction in cell viability after 48 hrs by SRB assay	[PMID: 28500966]
A549	IC₅₀	8.93 μg/mL Compound: Betunolic acid	Cytotoxicity against human A549 cells by MTT assay Cytotoxicity against human A549 cells by MTT assay	[PMID: 21106454]
Bcap37	IC₅₀	25.7 μM Compound: 1, BetA, Betulonic acid	Cytotoxicity against human Bcap37 cells after 72 hrs by MTT assay Cytotoxicity against human Bcap37 cells after 72 hrs by MTT assay	[PMID: 25874331]
BJ	IC₅₀	39.5 μM Compound: 5a	Cytotoxicity against human BJ cells incubated for 72 hrs by MTS assay Cytotoxicity against human BJ cells incubated for 72 hrs by MTS assay	[PMID: 31677446]
BRISTOL8	ED₅₀	2.6 μg/mL Compound: 13	Cytotoxic activity against human BRISTOL8 cell line was measured after 72 hr using MTT assay Cytotoxic activity against human BRISTOL8 cell line was measured after 72 hr using MTT assay	[PMID: 15225732]
BV-2	IC₅₀	> 100 μM Compound: 28	Anti-neuroinflammatory activity against mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay Anti-neuroinflammatory activity against mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay	[PMID: 25466192]
BV-2	IC₅₀	60.7 μM Compound: 1	Anti-neuroinflammatory activity in mouse BV-2 cells assessed as inhibition of LPS-induced NO production incubated for 48 hrs by Griess reagent based assay Anti-neuroinflammatory activity in mouse BV-2 cells assessed as inhibition of LPS-induced NO production incubated for 48 hrs by Griess reagent based assay	[PMID: 34546050]
Ca9-22	IC₅₀	14.47 μg/mL Compound: Betunolic acid	Cytotoxicity against human Ca9-22 cells by MTT assay Cytotoxicity against human Ca9-22 cells by MTT assay	[PMID: 21106454]
CCRF-CEM	IC₅₀	14 μM Compound: 2a	Cytotoxicity against human CCRF-CEM cells after 72 hrs by MTS assay Cytotoxicity against human CCRF-CEM cells after 72 hrs by MTS assay	[PMID: 27236068]
CCRF-CEM	IC₅₀	17 μM Compound: 7, betulonic acid	Cytotoxicity against human CEM cells after 72 hrs by MTT assay Cytotoxicity against human CEM cells after 72 hrs by MTT assay	[PMID: 15270560]
CCRF-CEM	IC₅₀	20 μM Compound: 3	Cytotoxicity against human CCRF-CEM cells after 72 hrs by MTT assay Cytotoxicity against human CCRF-CEM cells after 72 hrs by MTT assay	[PMID: 22551630]
CCRF-CEM	IC₅₀	20 μM Compound: 1b	Cytotoxicity against human CEM cells after 72 hrs by MTT assay Cytotoxicity against human CEM cells after 72 hrs by MTT assay	[PMID: 17371067]
CCRF-CEM	ED₅₀	4.4 μg/mL Compound: 13	Cytotoxic activity against human CEM.CM3 cell line was measured after 72 hr using MTT assay Cytotoxic activity against human CEM.CM3 cell line was measured after 72 hr using MTT assay	[PMID: 15225732]
CCRF-CEM	IC₅₀	8.4 μM Compound: 5a	Cytotoxicity against human CCRF-CEM cells incubated for 72 hrs by MTS assay Cytotoxicity against human CCRF-CEM cells incubated for 72 hrs by MTS assay	[PMID: 31677446]
CHO	EC₅₀	4.71 μM Compound: 7	Agonist activity at TGR5 expressed in CHO cells by CRE-driven luciferase reporter gene assay Agonist activity at TGR5 expressed in CHO cells by CRE-driven luciferase reporter gene assay	[PMID: 19911773]
COLO 205	IC₅₀	36.8 μM Compound: 17	Cytotoxicity against human COLON205 cells after 48 hrs by MTT assay Cytotoxicity against human COLON205 cells after 48 hrs by MTT assay	[PMID: 17346076]
COS-1	EC₅₀	0 μM Compound: 7	Agonist activity at human FXR expressed in COS1 cells by luciferase reporter gene assay Agonist activity at human FXR expressed in COS1 cells by luciferase reporter gene assay	[PMID: 19911773]
DU-145	IC₅₀	> 500 μM Compound: 9	Antiproliferative activity in human DU145 cells by sulforhodamine B assay Antiproliferative activity in human DU145 cells by sulforhodamine B assay	[PMID: 29120172]
DU-145	CC₅₀	139 μM Compound: 9	Cytotoxic activity in human DU145 cells by sulforhodamine B assay Cytotoxic activity in human DU145 cells by sulforhodamine B assay	[PMID: 29120172]
DU-145	ED₅₀	3.1 μg/mL Compound: 13	Cytotoxic activity against human DU145 cell line was measured after 72 hr using MTT assay Cytotoxic activity against human DU145 cell line was measured after 72 hr using MTT assay	[PMID: 15225732]
DU-145	IC₅₀	36 μM Compound: 7, betulonic acid	Cytotoxicity against human DU145 cells after 72 hrs by MTT assay Cytotoxicity against human DU145 cells after 72 hrs by MTT assay	[PMID: 15270560]
FaDu	EC₅₀	17.4 μM Compound: 2	Cytotoxicity against human FaDu cells assessed as reduction in cell viability incubated for 72 hrs by SRB assay Cytotoxicity against human FaDu cells assessed as reduction in cell viability incubated for 72 hrs by SRB assay	[PMID: 32956968]
Fibroblast	CC₅₀	> 100 μM Compound: 2	Cytotoxicity against mock-infected human embryonic lung fibroblast cells incubated for 5 days by MTS assay Cytotoxicity against mock-infected human embryonic lung fibroblast cells incubated for 5 days by MTS assay	[PMID: 33877845]
H9	EC₅₀	0.22 μg/mL Compound: 4	Antiviral activity against HIV1 3B infected in human H9 cells assessed as inhibition of viral p24 antigen levels after 4 days by ELISA Antiviral activity against HIV1 3B infected in human H9 cells assessed as inhibition of viral p24 antigen levels after 4 days by ELISA	[PMID: 11678650]
HCT-116	IC₅₀	> 10 μM Compound: 7	Cytotoxicity against human HCT116 cells assessed as reduction in cell viability after 48 hrs by SRB assay Cytotoxicity against human HCT116 cells assessed as reduction in cell viability after 48 hrs by SRB assay	[PMID: 28500966]
HCT-116	IC₅₀	2.61 μM Compound: 6	Antiproliferative activity against human HCT116 cells after 3 days by XTT assay Antiproliferative activity against human HCT116 cells after 3 days by XTT assay	[PMID: 19691293]
HCT-116	IC₅₀	38.1 μM Compound: 5a	Cytotoxicity against human HCT116 cells incubated for 72 hrs by MTS assay Cytotoxicity against human HCT116 cells incubated for 72 hrs by MTS assay	[PMID: 31677446]
HCT-116	IC₅₀	49.4 μM Compound: 5a	Cytotoxicity against p53 knockout human HCT116 cells incubated for 72 hrs by MTS assay Cytotoxicity against p53 knockout human HCT116 cells incubated for 72 hrs by MTS assay	[PMID: 31677446]
HEK293	CC₅₀	> 500 μM Compound: 9	Cytotoxic activity in HEK293 cells by sulforhodamine B assay Cytotoxic activity in HEK293 cells by sulforhodamine B assay	[PMID: 29120172]
HEK293	IC₅₀	> 500 μM Compound: 9	Antiproliferative activity in HEK293 cells by sulforhodamine B assay Antiproliferative activity in HEK293 cells by sulforhodamine B assay	[PMID: 29120172]
HeLa	CC₅₀	> 500 μM Compound: 9	Cytotoxic activity in human HeLa cells by sulforhodamine B assay Cytotoxic activity in human HeLa cells by sulforhodamine B assay	[PMID: 29120172]
HeLa	IC₅₀	> 500 μM Compound: 9	Antiproliferative activity in human HeLa cells by sulforhodamine B assay Antiproliferative activity in human HeLa cells by sulforhodamine B assay	[PMID: 29120172]
Hep 3B2	IC₅₀	13.81 μg/mL Compound: Betunolic acid	Cytotoxicity against human Hep3B cells by MTT assay Cytotoxicity against human Hep3B cells by MTT assay	[PMID: 21106454]
HEp-2	CC₅₀	> 500 μM Compound: 9	Cytotoxic activity in human Hep2 cells by sulforhodamine B assay Cytotoxic activity in human Hep2 cells by sulforhodamine B assay	[PMID: 29120172]
HEp-2	IC₅₀	> 500 μM Compound: 9	Antiproliferative activity in human Hep2 cells by sulforhodamine B assay Antiproliferative activity in human Hep2 cells by sulforhodamine B assay	[PMID: 29120172]
HepG2	CC₅₀	150.5 μM Compound: 4b	Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay	[PMID: 29326018]
HepG2	IC₅₀	4.32 μg/mL Compound: Betunolic acid	Cytotoxicity against human HepG2 cells by MTT assay Cytotoxicity against human HepG2 cells by MTT assay	[PMID: 21106454]
HT-29	IC₅₀	10.02 μM Compound: 2	Cytotoxicity against human HT-29 cells after 96 hrs by SRB colorimetric assay Cytotoxicity against human HT-29 cells after 96 hrs by SRB colorimetric assay	[PMID: 20472329]
HT-29	IC₅₀	17 μM Compound: 1b	Cytotoxicity against human HT29 cells after 72 hrs by MTT assay Cytotoxicity against human HT29 cells after 72 hrs by MTT assay	[PMID: 17371067]
HT-29	IC₅₀	17 μM Compound: 7, betulonic acid	Cytotoxicity against human HT-29 cells after 72 hrs by MTT assay Cytotoxicity against human HT-29 cells after 72 hrs by MTT assay	[PMID: 15270560]
HT-29	EC₅₀	18.5 μM Compound: 2	Cytotoxicity against human HT-29 cells assessed as reduction in cell viability incubated for 72 hrs by SRB assay Cytotoxicity against human HT-29 cells assessed as reduction in cell viability incubated for 72 hrs by SRB assay	[PMID: 32956968]
Jurkat	ED₅₀	0.4 μg/mL Compound: 13	Cytotoxic activity against human JurkatE6.1 cell line was measured after 72 h using MTT assay Cytotoxic activity against human JurkatE6.1 cell line was measured after 72 h using MTT assay	[PMID: 15225732]
K562	IC₅₀	13.6 μM Compound: 5a	Cytotoxicity against human K562 cells incubated for 72 hrs by MTS assay Cytotoxicity against human K562 cells incubated for 72 hrs by MTS assay	[PMID: 31677446]
K562	IC₅₀	16.1 μM Compound: 17	Cytotoxicity against human K562 cells after 48 hrs by MTT assay Cytotoxicity against human K562 cells after 48 hrs by MTT assay	[PMID: 17346076]
K562	IC₅₀	17 μM Compound: 7, betulonic acid	Cytotoxicity against paclitaxel-resistant human K562 cells after 72 hrs by MTT assay Cytotoxicity against paclitaxel-resistant human K562 cells after 72 hrs by MTT assay	[PMID: 15270560]
K562	IC₅₀	6 μM Compound: 1b	Cytotoxicity against human K562 cells after 72 hrs by MTT assay Cytotoxicity against human K562 cells after 72 hrs by MTT assay	[PMID: 17371067]
K562	IC₅₀	6 μM Compound: 7, betulonic acid	Cytotoxicity against human K562 cells after 72 hrs by MTT assay Cytotoxicity against human K562 cells after 72 hrs by MTT assay	[PMID: 15270560]
K562/Adr	IC₅₀	48.4 μM Compound: 17	Cytotoxicity against human doxorubicin-resistant K562/Adr cells in presence of 1 uM doxorubicin after 48 hrs by MTT assay Cytotoxicity against human doxorubicin-resistant K562/Adr cells in presence of 1 uM doxorubicin after 48 hrs by MTT assay	[PMID: 17346076]
K562/Adr	IC₅₀	73.5 μM Compound: 17	Cytotoxicity against human doxorubicin-resistant K562/Adr cells after 48 hrs by MTT assay Cytotoxicity against human doxorubicin-resistant K562/Adr cells after 48 hrs by MTT assay	[PMID: 17346076]
KB	CC₅₀	> 500 μM Compound: 9	Cytotoxic activity in human KB cells by sulforhodamine B assay Cytotoxic activity in human KB cells by sulforhodamine B assay	[PMID: 29120172]
KB	IC₅₀	> 500 μM Compound: 9	Antiproliferative activity in human KB cells by sulforhodamine B assay Antiproliferative activity in human KB cells by sulforhodamine B assay	[PMID: 29120172]
KB	IC₅₀	3.8 μM Compound: 17	Cytotoxicity against human KB cells after 48 hrs by MTT assay Cytotoxicity against human KB cells after 48 hrs by MTT assay	[PMID: 17346076]
L132	ED₅₀	> 10 μg/mL Compound: 13	Cytotoxic activity against human L132 cell line was measured after 72 hr using MTT assay Cytotoxic activity against human L132 cell line was measured after 72 hr using MTT assay	[PMID: 15225732]
MCF7	IC₅₀	> 10 μM Compound: 7	Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 48 hrs by SRB assay Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 48 hrs by SRB assay	[PMID: 28500966]
MCF7	CC₅₀	> 500 μM Compound: 9	Cytotoxic activity in human MCF7 cells by sulforhodamine B assay Cytotoxic activity in human MCF7 cells by sulforhodamine B assay	[PMID: 29120172]
MCF7	IC₅₀	> 500 μM Compound: 9	Antiproliferative activity in human MCF7 cells by sulforhodamine B assay Antiproliferative activity in human MCF7 cells by sulforhodamine B assay	[PMID: 29120172]
MCF7	IC₅₀	10.37 μM Compound: 2	Cytotoxicity against human MCF7 cells after 96 hrs by SRB colorimetric assay Cytotoxicity against human MCF7 cells after 96 hrs by SRB colorimetric assay	[PMID: 20472329]
MCF7	IC₅₀	10.42 μM Compound: 2	Cytotoxicity against human MCF7 cells assessed as cell viability after 96 hrs by SRB assay Cytotoxicity against human MCF7 cells assessed as cell viability after 96 hrs by SRB assay	[PMID: 23973824]
MCF7	EC₅₀	12 μM Compound: 2	Cytotoxicity against human MCF7 cells assessed as reduction in cell viability incubated for 72 hrs by SRB assay Cytotoxicity against human MCF7 cells assessed as reduction in cell viability incubated for 72 hrs by SRB assay	[PMID: 32956968]
MCF7	IC₅₀	18.2 μM Compound: 1, BetA, Betulonic acid	Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay	[PMID: 25874331]
MCF7	IC₅₀	19.32 μg/mL Compound: Betunolic acid	Cytotoxicity against human MCF7 cells by MTT assay Cytotoxicity against human MCF7 cells by MTT assay	[PMID: 21106454]
MCF7	EC₅₀	25.9 μM Compound: 4	Cytotoxicity against human MCF7 cells after 96 hrs by SRB assay Cytotoxicity against human MCF7 cells after 96 hrs by SRB assay	[PMID: 26547057]
MCF7	IC₅₀	29 μM Compound: 7, betulonic acid	Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay	[PMID: 15270560]
MCF7	IC₅₀	49 μM Compound: 17	Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay	[PMID: 17346076]
MDA-MB-231	IC₅₀	> 20 μg/mL Compound: Betunolic acid	Cytotoxicity against human MDA-MB-231 cells by MTT assay Cytotoxicity against human MDA-MB-231 cells by MTT assay	[PMID: 21106454]
MDA-MB-231	IC₅₀	> 500 μM Compound: 9	Antiproliferative activity in human MDA-MB-231 cells by sulforhodamine B assay Antiproliferative activity in human MDA-MB-231 cells by sulforhodamine B assay	[PMID: 29120172]
MDA-MB-231	CC₅₀	60.9 μM Compound: 9	Cytotoxic activity in human MDA-MB-231 cells by sulforhodamine B assay Cytotoxic activity in human MDA-MB-231 cells by sulforhodamine B assay	[PMID: 29120172]
MDA-MB-231	IC₅₀	9.77 μM Compound: 6	Antiproliferative activity against human MDA-MB-231 cells after 3 days by MTT assay Antiproliferative activity against human MDA-MB-231 cells after 3 days by MTT assay	[PMID: 19691293]
MGC-803	IC₅₀	17.7 μM Compound: 1, BetA, Betulonic acid	Cytotoxicity against human MGC803 cells after 72 hrs by MTT assay Cytotoxicity against human MGC803 cells after 72 hrs by MTT assay	[PMID: 25874331]
MIA PaCa-2	IC₅₀	> 10 μM Compound: 7	Cytotoxicity against human MIAPaCa2 cells assessed as reduction in cell viability after 48 hrs by SRB assay Cytotoxicity against human MIAPaCa2 cells assessed as reduction in cell viability after 48 hrs by SRB assay	[PMID: 28500966]
MOLT-4	ED₅₀	1.2 μg/mL Compound: 13	Cytotoxic activity against human MOLT-4 cell line was measured after 72 hr using MTT assay Cytotoxic activity against human MOLT-4 cell line was measured after 72 hr using MTT assay	[PMID: 15225732]
MRC5	IC₅₀	29.5 μM Compound: 5a	Cytotoxicity against human MRC5 cells incubated for 72 hrs by MTS assay Cytotoxicity against human MRC5 cells incubated for 72 hrs by MTS assay	[PMID: 31677446]
NIH3T3	EC₅₀	13.1 μM Compound: 4	Cytotoxicity against mouse NIH/3T3 cells after 96 hrs by SRB assay Cytotoxicity against mouse NIH/3T3 cells after 96 hrs by SRB assay	[PMID: 26547057]
NIH3T3	EC₅₀	19.4 μM Compound: 2	Cytotoxicity against mouse NIH3T3 cells assessed as reduction in cell viability incubated for 72 hrs by SRB assay Cytotoxicity against mouse NIH3T3 cells assessed as reduction in cell viability incubated for 72 hrs by SRB assay	[PMID: 32956968]
PA-1	ED₅₀	1.2 μg/mL Compound: 13	Cytotoxic activity against human PA-1 cell line was measured after 72 hr using MTT assay Cytotoxic activity against human PA-1 cell line was measured after 72 hr using MTT assay	[PMID: 15225732]
PC-3	IC₅₀	> 10 μM Compound: 7	Cytotoxicity against human PC3 cells assessed as reduction in cell viability after 48 hrs by SRB assay Cytotoxicity against human PC3 cells assessed as reduction in cell viability after 48 hrs by SRB assay	[PMID: 28500966]
PC-3	IC₅₀	13.9 μM Compound: 1, BetA, Betulonic acid	Cytotoxicity against human PC3 cells after 72 hrs by MTT assay Cytotoxicity against human PC3 cells after 72 hrs by MTT assay	[PMID: 25874331]
PC-3	IC₅₀	15 μM Compound: 1b	Cytotoxicity against human PC3 cells after 72 hrs by MTT assay Cytotoxicity against human PC3 cells after 72 hrs by MTT assay	[PMID: 17371067]
PC-3	IC₅₀	15 μM Compound: 7, betulonic acid	Cytotoxicity against human PC3 cells after 72 hrs by MTT assay Cytotoxicity against human PC3 cells after 72 hrs by MTT assay	[PMID: 15270560]
Platelet	IC₅₀	> 50 μg/mL Compound: Betunolic acid	Antiaggregatory activity in human platelets assessed as inhibition of thrombin-induced platelet aggregation by aggregometry Antiaggregatory activity in human platelets assessed as inhibition of thrombin-induced platelet aggregation by aggregometry	[PMID: 21106454]
Platelet	IC₅₀	44.71 μg/mL Compound: Betunolic acid	Antiaggregatory activity in human platelets assessed as inhibition of collagen-induced platelet aggregation by aggregometry Antiaggregatory activity in human platelets assessed as inhibition of collagen-induced platelet aggregation by aggregometry	[PMID: 21106454]
SiHa	IC₅₀	> 500 μM Compound: 9	Antiproliferative activity in human SiHa cells by sulforhodamine B assay Antiproliferative activity in human SiHa cells by sulforhodamine B assay	[PMID: 29120172]
SiHa	CC₅₀	116.8 μM Compound: 9	Cytotoxic activity in human SiHa cells by sulforhodamine B assay Cytotoxic activity in human SiHa cells by sulforhodamine B assay	[PMID: 29120172]
SK-MEL-2	IC₅₀	26 μM Compound: 1b	Cytotoxicity against human SK-MEL-2 cells after 72 hrs by MTT assay Cytotoxicity against human SK-MEL-2 cells after 72 hrs by MTT assay	[PMID: 17371067]
SK-MEL-2	IC₅₀	26 μM Compound: 7, betulonic acid	Cytotoxicity against human SK-MEL-2 cells after 72 hrs by MTT assay Cytotoxicity against human SK-MEL-2 cells after 72 hrs by MTT assay	[PMID: 15270560]
SW-1736	IC₅₀	3.05 μM Compound: 2	Cytotoxicity against human SW1736 cells after 96 hrs by SRB colorimetric assay Cytotoxicity against human SW1736 cells after 96 hrs by SRB colorimetric assay	[PMID: 20472329]
SW948	IC₅₀	19.17 μM Compound: 6	Antiproliferative activity against human SW948 cells after 3 days by XTT assay Antiproliferative activity against human SW948 cells after 3 days by XTT assay	[PMID: 19691293]
U2OS	IC₅₀	38 μM Compound: 5a	Cytotoxicity against human U2OS cells incubated for 72 hrs by MTS assay Cytotoxicity against human U2OS cells incubated for 72 hrs by MTS assay	[PMID: 31677446]
U-87MG ATCC	IC₅₀	159 μM Compound: 1b	Cytotoxicity against human U87-Mg cells after 72 hrs by MTT assay Cytotoxicity against human U87-Mg cells after 72 hrs by MTT assay	[PMID: 17371067]
U-937	ED₅₀	2.8 μg/mL Compound: 13	Cytotoxic activity against human U937 cell line was measured after 72 hr using MTT assay Cytotoxic activity against human U937 cell line was measured after 72 hr using MTT assay	[PMID: 15225732]
Vero	CC₅₀	> 400 μM Compound: 4b	Cytotoxicity against African green monkey Vero cells after 72 hrs by MTT assay Cytotoxicity against African green monkey Vero cells after 72 hrs by MTT assay	[PMID: 29326018]
Vero	CC₅₀	> 500 μM Compound: 9	Cytotoxic activity in African green monkey Vero cells by sulforhodamine B assay Cytotoxic activity in African green monkey Vero cells by sulforhodamine B assay	[PMID: 29120172]
Vero	IC₅₀	> 500 μM Compound: 9	Antiproliferative activity in African green monkey Vero cells by sulforhodamine B assay Antiproliferative activity in African green monkey Vero cells by sulforhodamine B assay	[PMID: 29120172]
Vero	EC₅₀	0.63 μM Compound: 14	Antiviral activity against SARS coronavirus in Vero E6 cells assessed as inhibition of viral replication by ELISA Antiviral activity against SARS coronavirus in Vero E6 cells assessed as inhibition of viral replication by ELISA	[PMID: 17663539]
Vero	CC₅₀	112 μM Compound: 14	Cytotoxicity against Vero E6 cells by MTT assay Cytotoxicity against Vero E6 cells by MTT assay	[PMID: 17663539]
Vero	CC₅₀	35.6 μM Compound: Betulonic acid	Cytotoxicity against African green monkey Vero cells assessed as cell viability after 2 days by trypan blue exclusion assay Cytotoxicity against African green monkey Vero cells assessed as cell viability after 2 days by trypan blue exclusion assay	[PMID: 26112446]

In Vitro

Betulonic acid (72 h) inhibits the growth of various types of human tumor cell lines, including MGC-803, PC3, Bcap-37, A375, MCF-7 tumor cell lines, with IC₅₀s of 17.7, 13.9, 25.7, 28.9, 18.2 μM, respectively^[2].
Betulonic acid has antiplasmodial activity, with IC₅₀ of 10 μM^[3].
Betulonic acid inhibits HSV-1, ECHO6 and influenza FPV viruses, with EC₅₀s of 0.9, 73.32, and 5.7 μM, respectively^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

454.68

Formula

C₃₀H₄₆O₃

CAS No.

4481-62-3

Appearance

Solid

Color

White to off-white

SMILES

CC1(C)C(CC[C@]2(C)[C@@]3([H])CC[C@]4([H])[C@@]5([H])[C@H](C(C)=C)CC[C@@](C(O)=O)5CC[C@](C)4[C@@](C)3CC[C@@]12[H])=O

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 5 mg/mL (11.00 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.1993 mL	10.9967 mL	21.9935 mL
5 mM	0.4399 mL	2.1993 mL	4.3987 mL
10 mM	0.2199 mL	1.0997 mL	2.1993 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 0.67 mg/mL (1.47 mM); Clear solution

This protocol yields a clear solution of ≥ 0.67 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (6.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 0.67 mg/mL (1.47 mM); Clear solution

This protocol yields a clear solution of ≥ 0.67 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (6.7 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.06%

Select Batch:

Data Sheet (278 KB) SDS (393 KB)

COA (254 KB) HNMR (296 KB) RP-HPLC (248 KB) MS (69 KB)

Handling Instructions (2659 KB)

References

[1]. Bastos DZL, et, al. Biotransformation of betulinic and betulonic acids by fungi. Phytochemistry. 2007 Mar;68(6):834-9. [Content Brief]

[2]. Yang SJ, et, al. Synthesis and biological evaluation of betulonic acid derivatives as antitumor agents. Eur J Med Chem. 2015;96:58-65. [Content Brief]

[3]. Sá MS, et, al. Antimalarial activity of betulinic acid and derivatives in vitro against Plasmodium falciparum and in vivo in P. berghei-infected mice. Parasitol Res. 2009 Jul;105(1):275-9. [Content Brief]

[4]. Pavlova NI, et, al. Antiviral activity of betulin, betulinic and betulonic acids against some enveloped and non-enveloped viruses. Fitoterapia. 2003 Jul;74(5):489-92. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.1993 mL	10.9967 mL	21.9935 mL	54.9837 mL
	5 mM	0.4399 mL	2.1993 mL	4.3987 mL	10.9967 mL
	10 mM	0.2199 mL	1.0997 mL	2.1993 mL	5.4984 mL

Betulonic acid Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Betulonic acid4481-62-3Betunolic acid Liquidambaric acid (+)-Betulonic acidParasiteHSVHerpes simplex virustriterpeneanti-tumoranti-inflammatoryantiparasiticanti-viralHSV-1Inhibitorinhibitorinhibit

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Betulonic acid (Synonyms: Betunolic acid; Liquidambaric acid; (+)-Betulonic acid)

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