2. Metabolic Enzyme/Protease
  3. Endogenous Metabolite
  4. Biocytin

Biocytin  (Synonyms: (+)-Biocytin)

Cat. No.: HY-101884 Purity: 99.52%
SDS COA Handling Instructions

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Biocytin is a conjugate of D-biotin and L-lysine, where the carboxylate of D-biotin is coupled with the ?-amine of L-lysine via a secondary amide bond. Biocytin is a classical neuroanatomical tracer commonly used to map brain connectivity. Biocytin is used as a versatile marker in anterograde, retrograde and intracellular neuroanatomical investigations and in biotinidase assays.

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Biocytin Chemical Structure

Biocytin Chemical Structure

CAS No. : 576-19-2

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
 USD 66 In-stock
Solution
10 mM * 1 mL in Water USD 66 In-stock
Solid
5 mg USD 38 In-stock
10 mg USD 60 In-stock
25 mg USD 84 In-stock
50 mg USD 108 In-stock
100 mg USD 162 In-stock
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Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of Biocytin:

Biocytin Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Biocytin is a conjugate of D-biotin and L-lysine, where the carboxylate of D-biotin is coupled with the ?-amine of L-lysine via a secondary amide bond. Biocytin is a classical neuroanatomical tracer commonly used to map brain connectivity. Biocytin is used as a versatile marker in anterograde, retrograde and intracellular neuroanatomical investigations and in biotinidase assays[1][2].

IC50 & Target

Human Endogenous Metabolite

 

In Vitro

Due to its high affinity for avidin, Biocytin can be visualized by using a host of avidin-conjugated markers at the light and electron microscopic level. Biocytin is used to visualize the morphology of dendritic and axonal arborizations[1].
The Biocytin wide-field bipolar cell in rabbit retina is a sparsely populated ON cone bipolar cell with a broad dendritic arbor that does not contact all cones in its dendritic field. Identification of bipolar cells by selective uptake of Biocytin, labeled the cones with peanut agglutinin, and then identification of cone types using antibodies against blue cone opsin and red-green cone opsin. The Biocytin-labeled cells selectively contact cones whose outer segments stained for blue cone opsin and avoided cones that do not. Biocytin wide-field bipolar cell is an ON blue cone bipolar cell in the rabbit retina and is homologous to the blue cone bipolar cells that have been previously described in primate, mouse, and ground squirrel retinas[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Biocytin Related Antibodies
Molecular Weight

372.48

Formula

C16H28N4O4S
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

N[C@@H](CCCCNC(CCCC[C@@H]1SC[C@]([C@]1([H])N2)([H])NC2=O)=O)C(O)=O
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

H2O : 50 mg/mL (134.24 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6847 mL 13.4235 mL 26.8471 mL
5 mM 0.5369 mL 2.6847 mL 5.3694 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (268.47 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
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g

Dosing volume
(per animal)

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.52%

SDS (393 KB)

COA (245 KB) HNMR (281 KB) LCMS (88 KB) Elemental Analysis Report (104 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 2.6847 mL 13.4235 mL 26.8471 mL 67.1177 mL
5 mM 0.5369 mL 2.6847 mL 5.3694 mL 13.4235 mL
10 mM 0.2685 mL 1.3424 mL 2.6847 mL 6.7118 mL
15 mM 0.1790 mL 0.8949 mL 1.7898 mL 4.4745 mL
20 mM 0.1342 mL 0.6712 mL 1.3424 mL 3.3559 mL
25 mM 0.1074 mL 0.5369 mL 1.0739 mL 2.6847 mL
30 mM 0.0895 mL 0.4475 mL 0.8949 mL 2.2373 mL
40 mM 0.0671 mL 0.3356 mL 0.6712 mL 1.6779 mL
50 mM 0.0537 mL 0.2685 mL 0.5369 mL 1.3424 mL
60 mM 0.0447 mL 0.2237 mL 0.4475 mL 1.1186 mL
80 mM 0.0336 mL 0.1678 mL 0.3356 mL 0.8390 mL
100 mM 0.0268 mL 0.1342 mL 0.2685 mL 0.6712 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Biocytin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Biocytin576-19-2(+)-BiocytinEndogenous MetaboliteInhibitorinhibitorinhibit

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