2. Immunology/Inflammation NF-κB Metabolic Enzyme/Protease Apoptosis
  3. Reactive Oxygen Species Ferroptosis
  4. Butylhydroxyanisole

Butylhydroxyanisole  (Synonyms: Butylated hydroxyanisole; BHA; E320)

Cat. No.: HY-B1066 Purity: 99.78%
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Butylhydroxyanisole (Butylated hydroxyanisole) is an antioxidant used as a food additive preservative. Butylhydroxyanisole mediates liver toxicity, retardation in reproductive organ development and learning, and sleep deficit. Butylhydroxyanisole exerts neurotoxic effects and leads to disruption of the brain and nerve development. Butylhydroxyanisole is a ferroptosis inducer.

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Butylhydroxyanisole Chemical Structure

Butylhydroxyanisole Chemical Structure

CAS No. : 25013-16-5

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10 mM * 1 mL in DMSO
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Based on 5 publication(s) in Google Scholar

Other Forms of Butylhydroxyanisole:

Butylhydroxyanisole Related Antibodies

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  • Purity & Documentation

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Description

Butylhydroxyanisole (Butylated hydroxyanisole) is an antioxidant used as a food additive preservative. Butylhydroxyanisole mediates liver toxicity, retardation in reproductive organ development and learning, and sleep deficit. Butylhydroxyanisole exerts neurotoxic effects and leads to disruption of the brain and nerve development[1][2][3]. Butylhydroxyanisole is a ferroptosis inducer[4].

In Vitro

Butylhydroxyanisole exerts neurotoxic effects by promoting cytosolic calcium accumulation and endoplasmic reticulum stress in astrocytes[1].
Butylhydroxyanisole (25-100 μM; 48 hours) inhibits growth and induces death in human astrocytes[1].
Butylhydroxyanisole (100 μM; 48 hours) decreases the expression of cell-cycle-related proteins and increased the expression of the cell cycle inhibitory protein[1].
Butylhydroxyanisole (100 μM; 48 hours) mediates apoptotic signals in NHA-SV40LT Cells[1].
Butylhydroxyanisole also increases the cytosolic calcium level and the expression of endoplasmic reticulum stress proteins[1].
Butylhydroxyanisole induces testicular dysfunction in mouse testis cells by dysregulating calcium homeostasis and stimulating endoplasmic reticulum stress[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Butylhydroxyanisole Related Antibodies

Cell Proliferation Assay[1]

Cell Line: NHA-SV40LT cells
Concentration: 0 μM, 25 μM, 50 μM, 75 μM, 100 μM
Incubation Time: 48 hours
Result: Exerted antiproliferative effects.

Cell Cycle Analysis[1]

Cell Line: NHA-SV40LT cells
Concentration: 100 μM
Incubation Time: 48 hours
Result: Downregulated the typical cell proliferative signaling pathways, phosphoinositide 3-kinase/protein kinase B and extracellular signal-regulated kinase 1/2.

Apoptosis Analysis[1]

Cell Line: NHA-SV40LT cells
Concentration: 100 μM
Incubation Time: 48 hours
Result: Increased the levels of pro-apoptotic proteins, such as BAX, cytochrome c, cleaved caspase 3, and cleaved caspase 9, and decreased the level of anti-apoptotic protein BCL-XL.

Western Blot Analysis[1]

Cell Line: NHA-SV40LT cells
Concentration: 100 μM
Incubation Time: 48 hours
Result: Increased the expression of pro-apoptotic proteins and decreased the levels of anti-apoptotic proteins. Asterisks show significant effects.
In Vivo

Butylhydroxyanisole (200 mg/kg; i.g.; daily; for three days) induces distinct expression patterns of Nrf2 and detoxification enzymes in the liver and small intestine of C57BL/6 mice[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Five-week-old C57BL/6 mice (WT and Nrf2-/-)[3]
Dosage: 200 mg/kg
Administration: Oral gavage, daily, for three days
Result: Increased Nqo1 staining in hepatocytes, predominately in the pericentral region.
Clinical Trial
Molecular Weight

180.24

Formula

C22H32O4
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

OC1=CC=C(OC)C=C1C(C)(C)C.OC2=CC=C(OC)C(C(C)(C)C)=C2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (554.82 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 1 mg/mL (5.55 mM; ultrasonic and warming and heat to 60°C)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 5.5482 mL 27.7408 mL 55.4816 mL
5 mM 1.1096 mL 5.5482 mL 11.0963 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (554.82 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 5.5482 mL 27.7408 mL 55.4816 mL 138.7039 mL
5 mM 1.1096 mL 5.5482 mL 11.0963 mL 27.7408 mL
DMSO 10 mM 0.5548 mL 2.7741 mL 5.5482 mL 13.8704 mL
15 mM 0.3699 mL 1.8494 mL 3.6988 mL 9.2469 mL
20 mM 0.2774 mL 1.3870 mL 2.7741 mL 6.9352 mL
25 mM 0.2219 mL 1.1096 mL 2.2193 mL 5.5482 mL
30 mM 0.1849 mL 0.9247 mL 1.8494 mL 4.6235 mL
40 mM 0.1387 mL 0.6935 mL 1.3870 mL 3.4676 mL
50 mM 0.1110 mL 0.5548 mL 1.1096 mL 2.7741 mL
60 mM 0.0925 mL 0.4623 mL 0.9247 mL 2.3117 mL
80 mM 0.0694 mL 0.3468 mL 0.6935 mL 1.7338 mL
100 mM 0.0555 mL 0.2774 mL 0.5548 mL 1.3870 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Butylhydroxyanisole Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Butylhydroxyanisole25013-16-5Butylated hydroxyanisole BHA E320E320E 320E-320Reactive Oxygen SpeciesFerroptosistoxicityneurotoxicantioxidantadditivepreservativetesticulardysfunctionstimulating endoplasmicreticulumstressendocrinedisruptorInhibitorinhibitorinhibit

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