2. Metabolic Enzyme/Protease
  3. Phospholipase
  4. CAY10650

CAY10650 is a highly potent cytosolic phospholipase A2α (cPLA2α) inhibitor with an IC50 value of 12 nM. CAY10650 suppresses lipid droplets formation and PGE2 secretion.

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CAY10650 Chemical Structure

CAY10650 Chemical Structure

CAS No. : 1233706-88-1

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10 mM * 1 mL in DMSO
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Customer Review

Based on 7 publication(s) in Google Scholar

CAY10650 Related Antibodies

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Description

CAY10650 is a highly potent cytosolic phospholipase A2α (cPLA2α) inhibitor with an IC50 value of 12 nM. CAY10650 suppresses lipid droplets formation and PGE2 secretion[1][2].

In Vitro

CAY10650 (12 nM; 30 min; neutrophils) inhibits the expression of the phosphorylated cPLA2-α (p-cPLA2-α) in cells[1].
CAY10650 (12 nM; 2 h; neutrophils) inhibits PGE2 release in neutrophils[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

CAY10650 Related Antibodies

Western Blot Analysis[1]

Cell Line: Neutrophils
Concentration: 12 nM
Incubation Time: 30 minutes
Result: Inhibited cPLA2-α and inhibited the expression of the p-cPLA2-α.

Western Blot Analysis[1]

Cell Line: Neutrophils
Concentration: 12 nM
Incubation Time: 2 hours
Result: Inhibited the PGE2 secretion and inhibited PGE2 realease.
In Vivo

CAY10650 (50 μg/5 μL; Inject with topical eye-drop; Chinese hamsters) relieves acanthamoeba keratitis in vivo.[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Chinese hamsters with infected with parasite-laden contact lenses[1]
Dosage: 50 μg/5 μL
Administration: Inject with topical eye-drop under the contact lens; three times a day for 6 days and topically on days 7 to 20 postinfection
Result: Reduced the severity of the keratitis and hasten the onset of resolution.
Had little mild inflammation and very few PMNs infiltration in the corneal stroma.
Molecular Weight

471.50

Formula

C28H25NO6
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(CN1C(C=CC(C(O)=O)=C2)=C2C(C(C(C)C)=O)=C1)COC(C=C3)=CC=C3OC4=CC=CC=C4
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (212.09 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1209 mL 10.6045 mL 21.2089 mL
5 mM 0.4242 mL 2.1209 mL 4.2418 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (5.30 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (5.30 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: ≥98.0%

SDS (252 KB)

COA (248 KB) HNMR (271 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.1209 mL 10.6045 mL 21.2089 mL 53.0223 mL
5 mM 0.4242 mL 2.1209 mL 4.2418 mL 10.6045 mL
10 mM 0.2121 mL 1.0604 mL 2.1209 mL 5.3022 mL
15 mM 0.1414 mL 0.7070 mL 1.4139 mL 3.5348 mL
20 mM 0.1060 mL 0.5302 mL 1.0604 mL 2.6511 mL
25 mM 0.0848 mL 0.4242 mL 0.8484 mL 2.1209 mL
30 mM 0.0707 mL 0.3535 mL 0.7070 mL 1.7674 mL
40 mM 0.0530 mL 0.2651 mL 0.5302 mL 1.3256 mL
50 mM 0.0424 mL 0.2121 mL 0.4242 mL 1.0604 mL
60 mM 0.0353 mL 0.1767 mL 0.3535 mL 0.8837 mL
80 mM 0.0265 mL 0.1326 mL 0.2651 mL 0.6628 mL
100 mM 0.0212 mL 0.1060 mL 0.2121 mL 0.5302 mL
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CAY10650 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

CAY106501233706-88-1CAY 10650CAY-10650Phospholipaselipid dropletsPGE2neutrophilsacanthamoebakeratitismannose-induced proteincorneal epithelial cellsInhibitorinhibitorinhibit

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