2. Metabolic Enzyme/Protease Apoptosis Anti-infection
  3. Cytochrome P450 Apoptosis Parasite
  4. Columbamine

Columbamine  (Synonyms: Columbamin; Dehydroisocorypalmine)

Cat. No.: HY-N0926 Purity: 98.38%
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COA
Handling Instructions Technical Support

Columbamine (Columbamin; Dehydroisocorypalmine) is an organic heterotetracyclic alkaloid extracted from plants. Columbamine is a metabolite of Berberine (HY-N0716). Columbamine inhibits the cytochrome P450 (CYP) isoform CYP3A4 (IC50 = 30.6 µM). Columbamine induces apoptosis in cancer cells. Columbamine can be used for antioxidant, anti-inflammatory, antitumor, antifungal, antiparasite, hepatoprotective, neuroprotective, hypolipidemic and hypoglycemic study.

For research use only. We do not sell to patients.

Columbamine Chemical Structure

Columbamine Chemical Structure

CAS No. : 3621-36-1

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Columbamine:

Columbamine Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Columbamine

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View All Isoforms
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Amebae Coccidia Leishmania Mite Plasmodium Toxoplasma Trypanosoma Schistosome

  • Biological Activity

  • Purity & Documentation

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  • Customer Review

Description

Columbamine (Columbamin; Dehydroisocorypalmine) is an organic heterotetracyclic alkaloid extracted from plants. Columbamine is a metabolite of Berberine (HY-N0716). Columbamine inhibits the cytochrome P450 (CYP) isoform CYP3A4 (IC50 = 30.6 µM). Columbamine induces apoptosis in cancer cells. Columbamine can be used for antioxidant, anti-inflammatory, antitumor, antifungal, antiparasite, hepatoprotective, neuroprotective, hypolipidemic and hypoglycemic study[1][2][3][4][5].

IC50 & Target

CYP3A4

30.6 μM (IC50)

In Vitro

Columbamine (M2) (15 μM, 24 h) decreases the intracellular triglyceride (TG) contents in Hep G2 cells[2].
Columbamine (15 μM, 24 h) induces a reduction of lipid synthesis and increases in fatty acid oxidation mediated via AMPK activation in Hep G2 cells[2].
Columbamine (10-50 μM, 24-72 h) inhibits the proliferation of colon cancer cells via suppressing Wnt/β-catenin signaling pathway[4].
Columbamine (20-40 μM, 24 h) inhibits the invasion and migration of colon cancer cells[4].
Columbamine (20-40 μM, 48 h) triggers apoptosis by inhibiting a caspase-dependent mechanism in colon cancer cells[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Columbamine Related Antibodies

Cell Proliferation Assay[4]

Cell Line: LoVo, HCT-116, SW480 (Human colorectal cancer cell lines)
Concentration: 10, 20, 30, 40, 50 μM
Incubation Time: 24, 48, 72 h
Result: Inhibited the growth of the colon cells, which were proportional to treatment time and dosage.
Suppressed clone formation of HCT116 cells in a concentration and time-dependent manner.

Cell Migration Assay [4]

Cell Line: LoVo, HCT-116
Concentration: 20, 30, 40 μM
Incubation Time: 24 h
Result: Significantly inhibited the migration and invasion of HCT116 and Lovo cells compared with the control cells.
Greatly impaired the invasion and migration of HCT116 cells in dose-dependent manner.
Suppressed the expression of N-Cadherin and promoted the expression of E-cadherin, resulting in the inhibition of epithelial-mesenchymal transition (EMT) of colon cancer cells.
Attenuated expressions of MMP2, MMP7 and MMP9 proteins.

Apoptosis Analysis[4]

Cell Line: HCT-116
Concentration: 20, 30, 40 μM
Incubation Time: 48 h
Result: Significantly prevented cell growth and promoted cell death at concentration from 20 μM to 40 μM.
Increased the population of Annexin V-positive cells.
The cleaved form of caspase-3 and PARP were increased without affecting the expression of caspase-3 and PARP.
Promoted pro-apoptosis factor BAD expression and repressed anti-apoptosis factor Bcl-2 expression.

RT-PCR[2]

Cell Line: Hep G2 cells
Concentration: 15 μM
Incubation Time: 24 h
Result: Inhibited the expression of genes, such as FAS, HMGR, ACC and GPAT, which are associated with fat synthesis.
Increased the gene expression of mCAD, but had no effects on CPT-1.
In Vivo

Columbamine (5-20 mg/kg; 23 days) significantly decreases the tumor volumes in a dose-dependent manner in HCT116 xenograft mice model[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Six-weeks-old male BALB/c nude mice (subcutaneously injected with HCT116 and control cells on the opposite flanks of the same mouse)[4]
Dosage: 5, 10, 20 mg/kg
Administration: Administered to experimental mice for 23 days
Result: Decreased the tumor volumes in a dose-dependent manner.
Molecular Weight

338.38

Formula

C20H20NO4
CAS No.
Appearance

Solid

Color

Light yellow to orange

SMILES

COC1=C(OC)C2=C[N+]3=C(C4=CC(O)=C(OC)C=C4CC3)C=C2C=C1
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : ≥ 23 mg/mL (67.97 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.9553 mL 14.7763 mL 29.5526 mL
5 mM 0.5911 mL 2.9553 mL 5.9105 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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(per animal)

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.70%

SDS (420 KB)

COA (249 KB) LCMS (94 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.9553 mL 14.7763 mL 29.5526 mL 73.8814 mL
5 mM 0.5911 mL 2.9553 mL 5.9105 mL 14.7763 mL
10 mM 0.2955 mL 1.4776 mL 2.9553 mL 7.3881 mL
15 mM 0.1970 mL 0.9851 mL 1.9702 mL 4.9254 mL
20 mM 0.1478 mL 0.7388 mL 1.4776 mL 3.6941 mL
25 mM 0.1182 mL 0.5911 mL 1.1821 mL 2.9553 mL
30 mM 0.0985 mL 0.4925 mL 0.9851 mL 2.4627 mL
40 mM 0.0739 mL 0.3694 mL 0.7388 mL 1.8470 mL
50 mM 0.0591 mL 0.2955 mL 0.5911 mL 1.4776 mL
60 mM 0.0493 mL 0.2463 mL 0.4925 mL 1.2314 mL
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Columbamine Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Columbamine3621-36-1Columbamin DehydroisocorypalmineCytochrome P450ApoptosisParasiteCYPsdehydroisocorypalmineantioxidantanti-inflammatoryantitumorantimicrobialhepatoprotectiveneuroprotectivehypolipidemichypoglycemictriglycerideHep G2Inhibitorinhibitorinhibit

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