2. Metabolic Enzyme/Protease Apoptosis Anti-infection
  3. Cytochrome P450 Apoptosis Parasite
  4. Columbamine chloride

Columbamine chloride  (Synonyms: Columbamin chloride; Dehydroisocorypalmine chloride)

Cat. No.: HY-N0926A Purity: 98.63%
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Columbamine (Columbamin; Dehydroisocorypalmine) chloride is an organic heterotetracyclic alkaloid extracted from plants. Columbamine chloride is a metabolite of Berberine (HY-N0716). Columbamine chloride inhibits the cytochrome P450 (CYP) isoform CYP3A4 (IC50 = 30.6 µM). Columbamine chloride induces apoptosis in cancer cells. Columbamine chloride can be used for antioxidant, anti-inflammatory, antitumor, antifungal, antiparasite, hepatoprotective, neuroprotective, hypolipidemic and hypoglycemic study.

For research use only. We do not sell to patients.

Columbamine chloride Chemical Structure

Columbamine chloride Chemical Structure

CAS No. : 1916-10-5

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Based on 1 publication(s) in Google Scholar

Other Forms of Columbamine chloride:

Columbamine chloride Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Columbamine chloride

View All Cytochrome P450 Isoform Specific Products:

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CYP1 CYP2 CYP3 CYP4 CYP11 CYP17 Aromatase/CYP19A1 CYP26 CYP51 CYP1A2 CYP2D6 CYP2C9 CYP2C19 CYP3A4 CYP17A1

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Amebae Coccidia Leishmania Mite Plasmodium Toxoplasma Trypanosoma Schistosome

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Description

Columbamine (Columbamin; Dehydroisocorypalmine) chloride is an organic heterotetracyclic alkaloid extracted from plants. Columbamine chloride is a metabolite of Berberine (HY-N0716). Columbamine chloride inhibits the cytochrome P450 (CYP) isoform CYP3A4 (IC50 = 30.6 µM). Columbamine chloride induces apoptosis in cancer cells. Columbamine chloride can be used for antioxidant, anti-inflammatory, antitumor, antifungal, antiparasite, hepatoprotective, neuroprotective, hypolipidemic and hypoglycemic study[1][2][3][4][5][6].

IC50 & Target

CYP3A4

30.6 μM (IC50)

Cellular Effect
Cell Line Type Value Description References
KB IC50
77.9 μM
Compound: Columbamine chloride
Cytotoxicity against human KB cells after 72 hrs
Cytotoxicity against human KB cells after 72 hrs
[PMID: 11141105]
In Vitro

Columbamine (M2) (15 μM, 24 h) chloride decreases the intracellular triglyceride (TG) contents in Hep G2 cells[2].
Columbamine (15 μM, 24 h) chloride induces a reduction of lipid synthesis and increases in fatty acid oxidation mediated via AMPK activation in Hep G2 cells[2].
Columbamine (10-50 μM, 24-72 h) chloride inhibits the proliferation of colon cancer cells via suppressing Wnt/β-catenin signaling pathway[4].
Columbamine (20-40 μM, 24 h) chloride inhibits the invasion and migration of colon cancer cells[4].
Columbamine (20-40 μM, 48 h) chloride triggers apoptosis by inhibiting a caspase-dependent mechanism in colon cancer cells[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Columbamine chloride Related Antibodies

Cell Proliferation Assay[4]

Cell Line: LoVo, HCT-116, SW480 (Human colorectal cancer cell lines)
Concentration: 10, 20, 30, 40, 50 μM
Incubation Time: 24, 48, 72 h
Result: Inhibited the growth of the colon cells, which were proportional to treatment time and dosage.
Suppressed clone formation of HCT116 cells in a concentration and time-dependent manner.

Cell Migration Assay [4]

Cell Line: LoVo, HCT-116
Concentration: 20, 30, 40 μM
Incubation Time: 24 h
Result: Significantly inhibited the migration and invasion of HCT116 and Lovo cells compared with the control cells.
Greatly impaired the invasion and migration of HCT116 cells in dose-dependent manner.
Suppressed the expression of N-Cadherin and promoted the expression of E-cadherin, resulting in the inhibition of epithelial-mesenchymal transition (EMT) of colon cancer cells.
Attenuated expressions of MMP2, MMP7 and MMP9 proteins.

Apoptosis Analysis[4]

Cell Line: HCT-116
Concentration: 20, 30, 40 μM
Incubation Time: 48 h
Result: Significantly prevented cell growth and promoted cell death at concentration from 20 μM to 40 μM.
Increased the population of Annexin V-positive cells.
The cleaved form of caspase-3 and PARP were increased without affecting the expression of caspase-3 and PARP.
Promoted pro-apoptosis factor BAD expression and repressed anti-apoptosis factor Bcl-2 expression.

RT-PCR[2]

Cell Line: Hep G2 cells
Concentration: 15 μM
Incubation Time: 24 h
Result: Inhibited the expression of genes, such as FAS, HMGR, ACC and GPAT, which are associated with fat synthesis.
Increased the gene expression of mCAD, but had no effects on CPT-1.
In Vivo

Columbamine (5-20 mg/kg; 23 days) significantly decreases the tumor volumes in a dose-dependent manner in HCT116 xenograft mice model[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Six-weeks-old male BALB/c nude mice (subcutaneously injected with HCT116 and control cells on the opposite flanks of the same mouse)[4]
Dosage: 5, 10, 20 mg/kg
Administration: Administered to experimental mice for 23 days
Result: Decreased the tumor volumes in a dose-dependent manner.
Molecular Weight

373.83

Formula

C20H20ClNO4
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

COC1=C(OC)C2=C[N+]3=C(C4=CC(O)=C(OC)C=C4CC3)C=C2C=C1.[Cl-]
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 5 mg/mL (13.38 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6750 mL 13.3751 mL 26.7501 mL
5 mM 0.5350 mL 2.6750 mL 5.3500 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.6750 mL 13.3751 mL 26.7501 mL 66.8753 mL
5 mM 0.5350 mL 2.6750 mL 5.3500 mL 13.3751 mL
10 mM 0.2675 mL 1.3375 mL 2.6750 mL 6.6875 mL
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Columbamine chloride Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Columbamine1916-10-5ColumbaminDehydroisocorypalmineCytochrome P450ApoptosisParasiteCYPsdehydroisocorypalmineantioxidantanti-inflammatoryantitumorantimicrobialhepatoprotectiveneuroprotectivehypolipidemichypoglycemictriglycerideHep G2Inhibitorinhibitorinhibit

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