Cyromazine (Synonyms: Cyromazin; CGA-72662)

Cat. No.: HY-B1331 Purity: 99.79%: FAQs
Data Sheet SDS COA Handling Instructions

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Cyromazine (CGA-72662) is a triazine insect growth regulator used as an insecticide and an acaricide. It is a cyclopropyl derivative of melamine. Cyromazine works by affecting the nervous system of the immature larval stages of certain insects. Cyromazine reduced the number of germ cells by interfering with the Ecdysone (HY-N0179) signaling pathway. Cyromazine induces renal toxicity attenuated and restored by green tea extract in rats.

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Cyromazine Chemical Structure

CAS No. : 66215-27-8

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10 mM * 1 mL in DMSO ready for reconstitution	USD 66	In-stock	Estimated Time of Arrival: December 31
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Other Forms of Cyromazine:

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Biological Activity
Purity & Documentation
References
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Description

IC₅₀ & Target

Human Endogenous Metabolite

In Vivo

Cyromazine (50 PPM, feed on a diet for 3 days) may decrease the number of germline stem cells (GSCs) and cystoblasts (CBs) in the 3-day old female adult ovary by affecting the expression of Ecdysone (HY-N0179) signaling-related genes, leading to a decrease fecundity^[2].
Cyromazine (169.35 mg/kg, oral gavage for 28 consecutive days) combined with Chlorpyrifos (HY-B0815) treated rats show huddling, mild tremor and diarrhea few minutes after dosing, but no manifestation is observed following aqueous green tea (GT) extract supplementation^[3].
Cyromazine (169.35 mg/kg, oral gavage for 28 consecutive days) combined with Chlorpyrifos (HY-B0815) treated rats ameliorates the loss of body weight and the increase of relative kidney after the administration of aqueous green tea (GT) extract^[3].
Cyromazine (50 PPM, feed on a diet for 7 days) on the inhibition effect of primordial germ cells (PGCs) is through the Ecdysone (HY-N0179) signaling pathway in the germ cells of larval ovaries^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	3-day-old female adult ovary of D. melanogaster^[2]^[3]
Dosage:	50 PPM
Administration:	feed on a diet for 3 days
Result:	Decreased the number of germline stem cells (GSCs) and cystoblasts (CBs) compared to the control group.

Molecular Weight

166.18

Formula

C₆H₁₀N₆

CAS No.

66215-27-8

Appearance

Solid

Color

White to off-white

SMILES

NC1=NC(N)=NC(NC2CC2)=N1

Structure Classification

Others

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 200 mg/mL (1203.51 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	6.0176 mL	30.0879 mL	60.1757 mL
5 mM	1.2035 mL	6.0176 mL	12.0351 mL
10 mM	0.6018 mL	3.0088 mL	6.0176 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (30.09 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (30.09 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 5 mg/mL (30.09 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.79%

Select Batch:

Data Sheet (274 KB) SDS (393 KB)

COA (246 KB) HNMR (244 KB) RP-HPLC (252 KB) LCMS (92 KB)

Handling Instructions (2659 KB)

References

[1]. Levot GW, et al. Survival advantage of cyromazine-resistant sheep blowfly larvae on dicyclanil- and cyromazine-treated Merinos. Aust Vet J. 2014 Nov;92(11):421-6. [Content Brief]

[2]. Khalid MZ, et al. Cyromazine Effects the Reproduction of Drosophila by Decreasing the Number of Germ Cells in the Female Adult Ovary[J]. Insects. 2022 Apr 27;13(5):414. [Content Brief]

[3]. T. Heikal, A. Mossa, et al. Cyromazine and Chlorpyrifos Induced Renal Toxicity in Rats: The Ameliorated Effects of Green Tea Extract[J]. Journal of Environmental and Analytical Toxicology. 2012 June 9; 2(5): 1000146.

[4]. Khalid MZ, et al. Cyromazine affects the ovarian germ cells of Drosophila via the ecdysone signaling pathway[J]. Front Physiol. 2022 Sep 29;13:992306. [Content Brief]

[5]. TAREK M.HEIKAL, et al. The ameliorating effects of green tea extract against cyromazine and chlorpyrifos induced liver toxicity in male rats[J]. Asian Journal of Pharmaceutical and Clinical Research. 2013 January; 6(1):47-55.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	6.0176 mL	30.0879 mL	60.1757 mL	150.4393 mL
	5 mM	1.2035 mL	6.0176 mL	12.0351 mL	30.0879 mL
	10 mM	0.6018 mL	3.0088 mL	6.0176 mL	15.0439 mL
	15 mM	0.4012 mL	2.0059 mL	4.0117 mL	10.0293 mL
	20 mM	0.3009 mL	1.5044 mL	3.0088 mL	7.5220 mL
	25 mM	0.2407 mL	1.2035 mL	2.4070 mL	6.0176 mL
	30 mM	0.2006 mL	1.0029 mL	2.0059 mL	5.0146 mL
	40 mM	0.1504 mL	0.7522 mL	1.5044 mL	3.7610 mL
	50 mM	0.1204 mL	0.6018 mL	1.2035 mL	3.0088 mL
	60 mM	0.1003 mL	0.5015 mL	1.0029 mL	2.5073 mL
	80 mM	0.0752 mL	0.3761 mL	0.7522 mL	1.8805 mL
	100 mM	0.0602 mL	0.3009 mL	0.6018 mL	1.5044 mL

Cyromazine Related Classifications

Neurological Disease Metabolic Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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