2. Neuronal Signaling
  3. Cholinesterase (ChE)
  4. Chlorpyrifos

Chlorpyrifos is a neurotoxic insecticide that belongs to the class of thionite esters. Chlorpyrifos is also a AChE inhibitor that affects neurological function in insects, humans and other animals. Chlorpyrifos interferes with cell replication and differentiation, ultimately altering synaptic transmission in neurons.

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Chlorpyrifos Chemical Structure

Chlorpyrifos Chemical Structure

CAS No. : 2921-88-2

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Based on 1 publication(s) in Google Scholar

Other Forms of Chlorpyrifos:

Chlorpyrifos Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Chlorpyrifos

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Description

Chlorpyrifos is a neurotoxic insecticide that belongs to the class of thionite esters. Chlorpyrifos is also a AChE inhibitor that affects neurological function in insects, humans and other animals. Chlorpyrifos interferes with cell replication and differentiation, ultimately altering synaptic transmission in neurons[1][2][3][4].

IC50 & Target

AChE

 

In Vitro

Chlorpyrifos mediates desulfuration to produce chlorpyrifos oxon (CPO), which has higher affinity toward the active site of serine-dependent ester hydrolases such as AChE[2].
Chlorpyrifos (3.9-250 μM; 24-72 h) is toxic to oligodendrocyte progenitors[3].
Chlorpyrifos toxicity is associated with (7.5-480 μM; 18 h) nuclear condensation and elevation of caspase 3/7 activity, (60 μM; 2, 4 h) Heme oxygenase-1 mRNA expression in Central Glia (CG-4) cells, and (30, 60, 120 μM; 24 h) enhances H2DCF-DA intensity[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chlorpyrifos Related Antibodies

Cell Viability Assay[3]

Cell Line: Oligodendrocyte CG-4 cells
Concentration: 3.9, 7.8, 15.6, 31.25, 62.5, 125, and 250 μM
Incubation Time: 24, 48, and 72 hours
Result: Significantly inhibited cell viability over 62.5 μM.

Immunofluorescence[3]

Cell Line: Oligodendrocyte CG-4 cells
Concentration: 0, 30, 60, 120 μM
Incubation Time: 24 hours
Result: Resulted nuclear condensation and elevation in a dose-dependent manner.
In Vivo

Chlorpyrifos (97-276 mg/kg; p.o.; single dose) has moderately acute oral toxicity, with lethal dose, 50% (LD50) of 97-276 mg/kg in rats[2].
Chlorpyrifos (1 mg/kg and 5 mg/kg for 1 mL/kg; s.c.; once daily for 3 d) has adverse effect on learning and memory abilities of rats, and affects pregnant rats on gestational days 9-12, results offspring behavioral abnormalities[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

350.59

Formula

C9H11Cl3NO3PS
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

S=P(OCC)(OCC)OC1=NC(Cl)=C(Cl)C=C1Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : 100 mg/mL (285.23 mM; Need ultrasonic)

DMSO : 50 mg/mL (142.62 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.8523 mL 14.2617 mL 28.5233 mL
5 mM 0.5705 mL 2.8523 mL 5.7047 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 6.25 mg/mL (17.83 mM); Clear solution

    This protocol yields a clear solution of ≥ 6.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (62.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 2.5 mg/mL (7.13 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.88%

COA (252 KB) LCMS (87 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 2.8523 mL 14.2617 mL 28.5233 mL 71.3084 mL
5 mM 0.5705 mL 2.8523 mL 5.7047 mL 14.2617 mL
10 mM 0.2852 mL 1.4262 mL 2.8523 mL 7.1308 mL
15 mM 0.1902 mL 0.9508 mL 1.9016 mL 4.7539 mL
20 mM 0.1426 mL 0.7131 mL 1.4262 mL 3.5654 mL
25 mM 0.1141 mL 0.5705 mL 1.1409 mL 2.8523 mL
30 mM 0.0951 mL 0.4754 mL 0.9508 mL 2.3769 mL
40 mM 0.0713 mL 0.3565 mL 0.7131 mL 1.7827 mL
50 mM 0.0570 mL 0.2852 mL 0.5705 mL 1.4262 mL
60 mM 0.0475 mL 0.2377 mL 0.4754 mL 1.1885 mL
80 mM 0.0357 mL 0.1783 mL 0.3565 mL 0.8914 mL
100 mM 0.0285 mL 0.1426 mL 0.2852 mL 0.7131 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Chlorpyrifos Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Chlorpyrifos2921-88-2Cholinesterase (ChE)Inhibitorinhibitorinhibit

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