2. Metabolic Enzyme/Protease
  3. Endogenous Metabolite
  4. DL-α-Tocopherol acetate

DL-α-Tocopherol acetate  (Synonyms: Vitamin E acetate)

Cat. No.: HY-B1278A Purity: 99.32%
COA Handling Instructions

Molarity Calculator

DL-α-Tocopherol acetate is a vitamin E derivative which is often included in the formulations of enteral nutrition.

For research use only. We do not sell to patients.

DL-α-Tocopherol acetate Chemical Structure

DL-α-Tocopherol acetate Chemical Structure

CAS No. : 52225-20-4

Size Price Stock Quantity
Oil + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 73 In-stock
Solution
10 mM * 1 mL in DMSO USD 73 In-stock
Oil
100 mg USD 66 In-stock
200 mg   Get quote  
500 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of DL-α-Tocopherol acetate:

DL-α-Tocopherol acetate Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

DL-α-Tocopherol acetate is a vitamin E derivative which is often included in the formulations of enteral nutrition.

In Vivo

DL-α-Tocopherol acetate is a vitamin E derivative which is often included in the formulations of enteral nutrition[1]. DL-α-Tocopherol acetate (α-TA) increases tissue cholesterol levels in liver, brian, testis and herart. The brain cholesterol level is much higher than those in other tissues when adjusted by protein level. DL-α-Tocopherol acetate also increases the level of lipid peroxidation in liver, brain, and testis of mice[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Clinical Trial
Molecular Weight

472.74

Formula

C31H52O3
CAS No.
Appearance

Oil

Color

Colorless to light yellow

SMILES

CC(CCC[C@H](CCC[C@H](CCC[C@@]1(CCC2=C(C(OC(C)=O)=C(C(C)=C2O1)C)C)C)C)C)C.CC(CCC[C@H](C)CCC[C@H](C)CCC[C@@]3(C)CCC4=C(C(OC(C)=O)=C(C(C)=C4O3)C)C)C
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Pure form -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

H2O : 100 mg/mL (211.53 mM; Need ultrasonic)

DMSO : ≥ 100 mg/mL (211.53 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1153 mL 10.5766 mL 21.1533 mL
5 mM 0.4231 mL 2.1153 mL 4.2307 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (5.29 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (5.29 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.32%

COA (248 KB) HNMR (89 KB) RP-HPLC (185 KB)

Handling Instructions (2659 KB)
References
Animal Administration
[2]

Male mice (specific-pathogen-free, 4 weeks of age) are divided into three groups. They are fed for 4 weeks with the CE2 diet containing one of three different antioxidants, 0.002 wt% DL-α-Tocopherol acetate (α-TA) (n=6), 0.02 wt% α-T (n=6) or 0.02 wt% α-TP(CD3) (n=7), per kilogram of diet. The mice are maintained under standardized conditions of light (7:00 AM to 7:00 PM), temperature (22°C), and humidity (70%). After 4 weeks, they are sacrificed under anesthesia with diethyl ether. The extracted brain, liver, heart, kidney, testis, and lung are homogenized in ice-cold 10 mM phosphate-buffered saline. Blood samples are collected in heparincontaining tubes. The samples are placed on ice immediately after collection. Plasma is obtained by centrifugation at 830 g for 5 min at 4°C and immediately subjected to analysis[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 2.1153 mL 10.5766 mL 21.1533 mL 52.8832 mL
5 mM 0.4231 mL 2.1153 mL 4.2307 mL 10.5766 mL
10 mM 0.2115 mL 1.0577 mL 2.1153 mL 5.2883 mL
15 mM 0.1410 mL 0.7051 mL 1.4102 mL 3.5255 mL
20 mM 0.1058 mL 0.5288 mL 1.0577 mL 2.6442 mL
25 mM 0.0846 mL 0.4231 mL 0.8461 mL 2.1153 mL
30 mM 0.0705 mL 0.3526 mL 0.7051 mL 1.7628 mL
40 mM 0.0529 mL 0.2644 mL 0.5288 mL 1.3221 mL
50 mM 0.0423 mL 0.2115 mL 0.4231 mL 1.0577 mL
60 mM 0.0353 mL 0.1763 mL 0.3526 mL 0.8814 mL
80 mM 0.0264 mL 0.1322 mL 0.2644 mL 0.6610 mL
100 mM 0.0212 mL 0.1058 mL 0.2115 mL 0.5288 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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DL-α-Tocopherol acetate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

DL-α-Tocopherol52225-20-4Vitamin EEndogenous MetaboliteInhibitorinhibitorinhibit

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