Enterostatin(human,mouse,rat)

Name: Enterostatin(human,mouse,rat)
Brand: MedChemExpress
SKU: HY-P1067
Rating: 4.5 (1 reviews)

Cat. No.: HY-P1067 Purity: 99.33%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Enterostatin, human, mouse, rat is a pentapeptide that reduces fat intake.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Enterostatin(human,mouse,rat) Chemical Structure

CAS No. : 117830-79-2

Size	Price	Stock	Quantity
5 mg	USD 180	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 270	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Based on 1 publication(s) in Google Scholar

Other Forms of Enterostatin(human,mouse,rat):

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Biological Activity
Purity & Documentation
References
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Description

Enterostatin, human, mouse, rat is a pentapeptide that reduces fat intake.

IC₅₀ & Target

Human Endogenous Metabolite

In Vitro

In the perfused ratpancreas, Enterostatin, at 100 mM, inhibits the insulin response to 9 mM glucose (by 70%), 0.1 mM tolbutamide (by 40%), and 5 mM arginine (by 70%)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Chronically, enterostatin reduces fat intake, bodyweight, and body fat. This response may involve multiple metabolic effects of enterostatin, which include a reduction of insulin secretion, an increase in sympathetic drive to brown adipose tissue, and the stimulation of adrenal corticosteroid secretion^[2]. Enterostatin enhances memory consolidation after central or oral administration at a dose of 10 nmol/mouse or 300 mg/kg, respectively, in a step-through type passive avoidance test in mice^[3]. A dose of 38 nmol of enterostatin gives a significant inhibition of high-fat food intake, while at a higher dose of 76 nmol the inhibiting effect is lost. During the first hour, after injection of enterostatin, there is even a slight increase in food intake^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

496.56

Formula

C₂₁H₃₆N₈O₆

CAS No.

117830-79-2

Appearance

Solid

Color

White to off-white

Sequence

Ala-Pro-Gly-Pro-Arg

Sequence Shortening

APGPR

Structure Classification

Others

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (251.73 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.0139 mL	10.0693 mL	20.1386 mL
5 mM	0.4028 mL	2.0139 mL	4.0277 mL
10 mM	0.2014 mL	1.0069 mL	2.0139 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (4.19 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (4.19 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.33%

Select Batch:

Data Sheet (273 KB) SDS (252 KB)

COA (247 KB) RP-HPLC (111 KB) MS (219 KB) Elemental Analysis Report (107 KB)

Handling Instructions (2659 KB)

References

[1]. Silvestre RA, et al. Effect of enterostatin on insulin, glucagon, and somatostatin secretion in the perfused rat pancreas. Diabetes. 1996 Sep;45(9):1157-60. [Content Brief]

[2]. Erlanson-Albertsson C, et al. Enterostatin--a peptide regulating fat intake. Obes Res. 1997 Jul;5(4):360-72. [Content Brief]

[3]. Ohinata K, et al. Enterostatin (APGPR) enhances memory consolidation in mice. Peptides. 2007 Mar;28(3):719-21. [Content Brief]

[4]. Sörhede M, et al. Enterostatin: a gut-brain peptide regulating fat intake in rat. J Physiol Paris. 1993;87(4):273-5. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.0139 mL	10.0693 mL	20.1386 mL	50.3464 mL
	5 mM	0.4028 mL	2.0139 mL	4.0277 mL	10.0693 mL
	10 mM	0.2014 mL	1.0069 mL	2.0139 mL	5.0346 mL
	15 mM	0.1343 mL	0.6713 mL	1.3426 mL	3.3564 mL
	20 mM	0.1007 mL	0.5035 mL	1.0069 mL	2.5173 mL
	25 mM	0.0806 mL	0.4028 mL	0.8055 mL	2.0139 mL
	30 mM	0.0671 mL	0.3356 mL	0.6713 mL	1.6782 mL
	40 mM	0.0503 mL	0.2517 mL	0.5035 mL	1.2587 mL
	50 mM	0.0403 mL	0.2014 mL	0.4028 mL	1.0069 mL
	60 mM	0.0336 mL	0.1678 mL	0.3356 mL	0.8391 mL
	80 mM	0.0252 mL	0.1259 mL	0.2517 mL	0.6293 mL
	100 mM	0.0201 mL	0.1007 mL	0.2014 mL	0.5035 mL