2. GPCR/G Protein
  3. Prostaglandin Receptor
  4. GW627368

GW627368 (GW627368X) is a novel, potent and selective competitive antagonist of prostanoid EP4 receptor with additional human TP receptor affinity, with pKi values of 7.0 and 6.8 for human prostanoid EP4 and TP receptors respectively.

For research use only. We do not sell to patients.

GW627368 Chemical Structure

GW627368 Chemical Structure

CAS No. : 439288-66-1

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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10 mM * 1 mL in DMSO USD 79 In-stock
Solid
5 mg USD 66 In-stock
10 mg USD 99 In-stock
25 mg USD 165 In-stock
50 mg USD 275 In-stock
100 mg USD 495 In-stock
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Customer Review

Based on 9 publication(s) in Google Scholar

GW627368 Related Antibodies

Top Publications Citing Use of Products

    GW627368 purchased from MedChemExpress. Usage Cited in: J Cell Physiol. 2018 Nov;233(11):8984-8995.  [Abstract]

    Small white follicles (SWFs) are treated with TG6-10-1 or GW627368 and WB are used and gray analysis of PCNA, CDK2, and CCND1.

    GW627368 purchased from MedChemExpress. Usage Cited in: Oncol Lett. 2018 Jan;15(1):552-558.  [Abstract]

    The level of PD 1 expression is subsequently detected by western blot analysis. Data is presented as the mean±standard deviation. P<0.05. The control group is treated with PGE2, but not with an antagonist.

    View All Prostaglandin Receptor Isoform Specific Products:

    View All Isoforms
    DP EP FP IP TXA2/TP TXB2

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    GW627368 (GW627368X) is a novel, potent and selective competitive antagonist of prostanoid EP4 receptor with additional human TP receptor affinity, with pKi values of 7.0 and 6.8 for human prostanoid EP4 and TP receptors respectively[1].

    IC50 & Target

    EP

     

    In Vitro

    GW627368 (GW627368X) appears to bind to human prostanoid TP receptors but not the TP receptors of other species[1].
    GW627368 (GW627368X) (10 μM) produces 100% inhibition of U-46619 (EC100)-induced aggregation (approximate pA2 approximately 7.0) in human washed platelets[1].
    GW627368 (GW627368X) is devoid of agonist activity and actually produced a significant and concentration-related reduction in basal cAMP levels with pIC50 value of 6.3[1].
    GW627368 (GW627368X) induces inhibition of proliferation and invasion of human SUM149 IBC tumor cells beginning at 0.1 μM, with inhibition of proliferation and invasion of MDA-MB-231 non-IBC cells at higher concentrations[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    GW627368 Related Antibodies
    In Vivo

    GW627368 (GW627368X) (0-15 mg/kg; p.o.; every alternate day for 28 days) shows significant tumor regression characterized by tumor reduction and induction of apoptosis[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: 6-8 weeks Swiss albino mice[3]
    Dosage: 0 mg/kg, 5 mg/kg, 10 mg/kg, 15 mg/kg, 15 mg/kg
    Administration: Oral administration, every alternate day for 28 days
    Result: Displayed anti-tumor and anti-proliferative potential in sarcoma 180 bearing mice.
    Molecular Weight

    544.62

    Formula

    C30H28N2O6S
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C(NS(=O)(C1=CC=CC=C1)=O)CC2=CC=C(N(CC3=C4C(OCC)=C(C=CC=C5)C5=C3OCC)C4=O)C=C2
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (183.61 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.8361 mL 9.1807 mL 18.3614 mL
    5 mM 0.3672 mL 1.8361 mL 3.6723 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Volume
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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    Volume (start)

    V1

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    C2

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    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (4.59 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 2.5 mg/mL (4.59 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

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    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.81%

    SDS (393 KB)

    COA (251 KB) LCMS (266 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.8361 mL 9.1807 mL 18.3614 mL 45.9036 mL
    5 mM 0.3672 mL 1.8361 mL 3.6723 mL 9.1807 mL
    10 mM 0.1836 mL 0.9181 mL 1.8361 mL 4.5904 mL
    15 mM 0.1224 mL 0.6120 mL 1.2241 mL 3.0602 mL
    20 mM 0.0918 mL 0.4590 mL 0.9181 mL 2.2952 mL
    25 mM 0.0734 mL 0.3672 mL 0.7345 mL 1.8361 mL
    30 mM 0.0612 mL 0.3060 mL 0.6120 mL 1.5301 mL
    40 mM 0.0459 mL 0.2295 mL 0.4590 mL 1.1476 mL
    50 mM 0.0367 mL 0.1836 mL 0.3672 mL 0.9181 mL
    60 mM 0.0306 mL 0.1530 mL 0.3060 mL 0.7651 mL
    80 mM 0.0230 mL 0.1148 mL 0.2295 mL 0.5738 mL
    100 mM 0.0184 mL 0.0918 mL 0.1836 mL 0.4590 mL
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    GW627368 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    GW627368439288-66-1GW 627368GW-627368Prostaglandin ReceptorInhibitorinhibitorinhibit

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