2. Metabolic Enzyme/Protease
  3. Endogenous Metabolite
  4. Glycerol

Glycerol  (Synonyms: Trihydroxypropane)

Cat. No.: HY-B1659 Purity: 99.93%
COA Handling Instructions

Molarity Calculator

Glycerol is used in sample preparation and gel formation for polyacrylamide gel electrophoresis.

For research use only. We do not sell to patients.

Glycerol Chemical Structure

Glycerol Chemical Structure

CAS No. : 56-81-5

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Based on 1 publication(s) in Google Scholar

Other Forms of Glycerol:

Glycerol Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

Glycerol is used in sample preparation and gel formation for polyacrylamide gel electrophoresis.

IC50 & Target

Human Endogenous Metabolite

 

In Vitro

Glycerol is often included in polyacrylamide gels to prevent dissociation of nucleosomes and other protein-DNA complexes during electrophoresis. With glycerol included, fractionation seems to be largely based on particle mass and charge. The concentration of glycerol during electrophoresis strongly affects the separation characteristics of polyacrylamide gels[1]. Glycerol is an inevitable by-product of oils/fats processing, regardless of the pathway. Fermentative metabolism of glycerol has been studied in great detail in several species of the Enterobacteriaceae family, such as Citrobacter freundii and Klebsiella pneumoniae. The use of anaerobic fermentation to convert abundant and low-priced glycerol streams generated in the production of biodiesel into higher value products represents a promising route to achieve economic viability in the biofuels industry[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Glycerol Related Antibodies
In Vivo

Glycerol can induce acute renal failure in rat models. Acute renal failure induced by glycerol or uranyl nitrate reduces the hepato-biliary transport of some drugs, modulates the distribution of drugs into the central nervous system and affects the activity of various hepatic microsomal enzymes [3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

92.09

Formula

C3H8O3
CAS No.
Appearance

Liquid (Density: 1.26 g/cm3)

Color

Colorless to light yellow

SMILES

OCC(O)CO
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Store at room temperature 3 years

In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (1085.89 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 100 mg/mL (1085.89 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 10.8589 mL 54.2947 mL 108.5894 mL
5 mM 2.1718 mL 10.8589 mL 21.7179 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 3.79 mg/mL (41.16 mM); Clear solution

    This protocol yields a clear solution of ≥ 3.79 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (37.9 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (22.59 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (1085.89 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.99%

COA (243 KB) HNMR (116 KB) GC (250 KB)

Handling Instructions (2659 KB)
References
Animal Administration
[3]

Rats: Experimental acute renal failure was induced in male Wistar rats 230-300 g by an injection of glycerol dissolved in saline (50% v/v, 10 mL/kg) into the leg muscle after a 24-h period of water deprivation[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 10.8589 mL 54.2947 mL 108.5894 mL 271.4736 mL
5 mM 2.1718 mL 10.8589 mL 21.7179 mL 54.2947 mL
10 mM 1.0859 mL 5.4295 mL 10.8589 mL 27.1474 mL
15 mM 0.7239 mL 3.6196 mL 7.2393 mL 18.0982 mL
20 mM 0.5429 mL 2.7147 mL 5.4295 mL 13.5737 mL
25 mM 0.4344 mL 2.1718 mL 4.3436 mL 10.8589 mL
30 mM 0.3620 mL 1.8098 mL 3.6196 mL 9.0491 mL
40 mM 0.2715 mL 1.3574 mL 2.7147 mL 6.7868 mL
50 mM 0.2172 mL 1.0859 mL 2.1718 mL 5.4295 mL
60 mM 0.1810 mL 0.9049 mL 1.8098 mL 4.5246 mL
80 mM 0.1357 mL 0.6787 mL 1.3574 mL 3.3934 mL
100 mM 0.1086 mL 0.5429 mL 1.0859 mL 2.7147 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Glycerol Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Glycerol56-81-5TrihydroxypropaneEndogenous MetaboliteInhibitorinhibitorinhibit

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