IDO-IN-1

Cat. No.: HY-79531 Purity: 98.13%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

IDO-IN-1 is a potent indoleamine 2,3-dioxygenase (IDO) inhibitor with an IC₅₀ of 59 nM.

For research use only. We do not sell to patients.

IDO-IN-1 Chemical Structure

CAS No. : 914638-30-5

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 243	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 243	In-stock	Estimated Time of Arrival: December 31
Solid
2 mg	USD 140	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 210	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 336	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 604	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 907	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 1269	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IDO-IN-1 is a potent indoleamine 2,3-dioxygenase (IDO) inhibitor with an IC₅₀ of 59 nM.

IC₅₀ & Target^[1]

IDO

59 nM (IC₅₀)

IDO

12 nM (IC₅₀, in HeLa cell)

In Vitro

IDO-IN-1 (Compound 5m) is a potent (HeLa IC₅₀=12 nM) inhibitor of IDO^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

316.09

Formula

C₉H₇BrFN₅O₂

CAS No.

914638-30-5

Appearance

Solid

Color

White to gray

SMILES

NC1=NON=C1/C(NO)=N/C2=CC(Br)=C(F)C=C2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 120 mg/mL (379.64 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.1637 mL	15.8183 mL	31.6366 mL
5 mM	0.6327 mL	3.1637 mL	6.3273 mL
10 mM	0.3164 mL	1.5818 mL	3.1637 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 3 mg/mL (9.49 mM); Clear solution

This protocol yields a clear solution of ≥ 3 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (30.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 3 mg/mL (9.49 mM); Clear solution

This protocol yields a clear solution of ≥ 3 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (30.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 3 mg/mL (9.49 mM); Clear solution

This protocol yields a clear solution of ≥ 3 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (30.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.13%

Select Batch:

Data Sheet (273 KB) SDS (393 KB)

COA (251 KB) LCMS (96 KB)

Handling Instructions (2659 KB)

References

[1]. Yue EW, et al. Discovery of potent competitive inhibitors of indoleamine 2,3-dioxygenase with in vivo pharmacodynamic activity and efficacy in a mouse melanoma model. J Med Chem. 2009 Dec 10;52(23):7364-7. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.1637 mL	15.8183 mL	31.6366 mL	79.0914 mL
	5 mM	0.6327 mL	3.1637 mL	6.3273 mL	15.8183 mL
	10 mM	0.3164 mL	1.5818 mL	3.1637 mL	7.9091 mL
	15 mM	0.2109 mL	1.0546 mL	2.1091 mL	5.2728 mL
	20 mM	0.1582 mL	0.7909 mL	1.5818 mL	3.9546 mL
	25 mM	0.1265 mL	0.6327 mL	1.2655 mL	3.1637 mL
	30 mM	0.1055 mL	0.5273 mL	1.0546 mL	2.6364 mL
	40 mM	0.0791 mL	0.3955 mL	0.7909 mL	1.9773 mL
	50 mM	0.0633 mL	0.3164 mL	0.6327 mL	1.5818 mL
	60 mM	0.0527 mL	0.2636 mL	0.5273 mL	1.3182 mL
	80 mM	0.0395 mL	0.1977 mL	0.3955 mL	0.9886 mL
	100 mM	0.0316 mL	0.1582 mL	0.3164 mL	0.7909 mL

IDO-IN-1 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

IDO-IN-1914638-30-5Indoleamine 2,3-Dioxygenase (IDO)Inhibitorinhibitorinhibit

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