KN-93 phosphate

Name: KN-93 phosphate
Brand: MedChemExpress
SKU: HY-15465B
Rating: 5.0 (67 reviews)

Cat. No.: HY-15465B Purity: 99.67%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

KN-93 phosphate is a novel membrane-permeant synthetic inhibitor of purified neuronal CaMK-II, with K_i of 370 nM.

For research use only. We do not sell to patients.

KN-93 phosphate Chemical Structure

CAS No. : 1913269-12-1

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 174	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 174	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	USD 66	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 132	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 180	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 720	In-stock	Estimated Time of Arrival: December 31
100 mg		Get quote
200 mg		Get quote

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Customer Review

Based on 67 publication(s) in Google Scholar

Other Forms of KN-93 phosphate:

KN-93 In-stock
KN-93 hydrochloride In-stock
(E)-KN-93 phosphate Get quote

63 Publications Citing Use of MCE KN-93 phosphate

: KN-93 phosphate purchased from MedChemExpress. Usage Cited in: Acta Pharmacol Sin. 2020 Feb;41(2):218-228. [Abstract]; HUVECs are pretreated with KN93 for 1 h, followed by ZYZ-803 (1 μM) for 30 min. Protein levels are assessed by Western blot.

: KN-93 phosphate purchased from MedChemExpress. Usage Cited in: Diabetes. 2018 Sep;67(9):1748-1760. [Abstract]; Immunoblotting analysis of PCK1, G6Pase, and phospho-CaMKIIγ proteins in NTG and HCTG hepatocytes in response to PGF2α and in the presence or absence of KN-93 treatment.

: KN-93 phosphate purchased from MedChemExpress. Usage Cited in: Front Pharmacol. 2018 Apr 12;9:362. [Abstract]; MK 733-increased biotinylated α7nAChR protein is blocked by the addition of KN93 (P<0.01, n=6 mice).

: KN-93 phosphate purchased from MedChemExpress. Usage Cited in: Exp Cell Res. 2018 Mar 15;364(2):198-207. [Abstract]; Western blot analysis of β-catenin protein level in HEK 293T cells stimulated for 12h with rAGR2 (100 ng/mL) coupled with or without KN93 (10 μM).

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Biological Activity
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References
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Description

KN-93 phosphate is a novel membrane-permeant synthetic inhibitor of purified neuronal CaMK-II, with K_i of 370 nM.

IC₅₀ & Target

CaMK II

In Vitro

After 2 days of KN-93 treatment, 95% of cells are arrested in G1. G1 arrest is reversible; 1 day after KN-93 release, a peak of cells had progressed into S and G2-M. KN-93 also blocks cell growth stimulated by basic fibroblast growth factor, platelet-derived growth factor-BB, and epidermal growth factor in NIH 3T3 fibroblasts^[1]. KN-93 inhibits the H⁺, K⁺-ATPase activity but strongly dissipates the proton gradient formed in the gastric membrane vesicles and reduces the volume of luminal space^[2]. KN-93 (0.5 μM) prevents increased LV developed pressure during action potential prolongation and early afterdepolarizations. Ca²⁺-independent CaM kinase activity is increased during early afterdepolarizations and this increase is prevented by KN-93^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

599.03

Formula

C₂₆H₃₂ClN₂O₈PS

CAS No.

1913269-12-1

Appearance

Solid

Color

White to off-white

SMILES

OP(O)(O)=O.O=S(C1=CC=C(OC)C=C1)(N(C2=CC=CC=C2CN(C/C=C/C3=CC=C(Cl)C=C3)C)CCO)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (166.94 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 50 mg/mL (83.47 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.6694 mL	8.3468 mL	16.6937 mL
5 mM	0.3339 mL	1.6694 mL	3.3387 mL
10 mM	0.1669 mL	0.8347 mL	1.6694 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 10 mg/mL (16.69 mM); Clear solution

This protocol yields a clear solution of ≥ 10 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (100.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 10 mg/mL (16.69 mM); Clear solution

This protocol yields a clear solution of ≥ 10 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (100.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 10 mg/mL (16.69 mM); Clear solution

This protocol yields a clear solution of ≥ 10 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (100.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.67%

Select Batch:

Data Sheet (281 KB) SDS (393 KB)

COA (258 KB) LCMS (93 KB)

Handling Instructions (2659 KB)

References

[1]. Tombes RM, et al. G1 cell cycle arrest and apoptosis are induced in NIH 3T3 cells by KN-93, an inhibitor of CaMK-II (the multifunctional Ca2+/CaM kinase). Cell Growth Differ. 1995 Sep;6(9):1063-70. [Content Brief]

[2]. Li J, et al. Curcumin Attenuates Retinal Vascular Leakage by Inhibiting Calcium/Calmodulin-Dependent Protein Kinase II Activity in Streptozotocin-Induced Diabetes. Cell Physiol Biochem. 2016;39(3):1196-208. [Content Brief]

[3]. Mamiya N, et al. Inhibition of acid secretion in gastric parietal cells by the Ca2+/calmodulin-dependent protein kinase II inhibitorKN-93. Biochem Biophys Res Commun. 1993 Sep 15;195(2):608-15. [Content Brief]

[4]. Anderson ME, et al. KN-93, an inhibitor of multifunctional Ca++/calmodulin-dependent protein kinase, decreases early afterdepolarizations in rabbit heart. J Pharmacol Exp Ther. 1998 Dec;287(3):996-1006. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	1.6694 mL	8.3468 mL	16.6937 mL	41.7341 mL
	5 mM	0.3339 mL	1.6694 mL	3.3387 mL	8.3468 mL
	10 mM	0.1669 mL	0.8347 mL	1.6694 mL	4.1734 mL
	15 mM	0.1113 mL	0.5565 mL	1.1129 mL	2.7823 mL
	20 mM	0.0835 mL	0.4173 mL	0.8347 mL	2.0867 mL
	25 mM	0.0668 mL	0.3339 mL	0.6677 mL	1.6694 mL
	30 mM	0.0556 mL	0.2782 mL	0.5565 mL	1.3911 mL
	40 mM	0.0417 mL	0.2087 mL	0.4173 mL	1.0434 mL
	50 mM	0.0334 mL	0.1669 mL	0.3339 mL	0.8347 mL
	60 mM	0.0278 mL	0.1391 mL	0.2782 mL	0.6956 mL
	80 mM	0.0209 mL	0.1043 mL	0.2087 mL	0.5217 mL
DMSO	100 mM	0.0167 mL	0.0835 mL	0.1669 mL	0.4173 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

KN-93 phosphate Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

KN-931913269-12-1KN93KN 93CaMKAutophagyCalmodulin-dependent protein kinasesCalmodulin-dependent kinasesInhibitorinhibitorinhibit

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KN-93 phosphate

Customer Review

Other Forms of KN-93 phosphate:

KN-93 phosphate Related Antibodies

63 Publications Citing Use of MCE KN-93 phosphate

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All CaMK Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

KN-93 phosphate Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

KN-93 phosphate Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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