2. Stem Cell/Wnt TGF-beta/Smad
  3. PKA
  4. Kemptide

Kemptide is a synthetic heptapeptide that acts as a specific substrate for cAMP-dependent protein kinase (PKA).

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Kemptide Chemical Structure

Kemptide Chemical Structure

CAS No. : 65189-71-1

Size Price Stock Quantity
1 mg USD 60 In-stock
5 mg USD 90 In-stock
10 mg USD 145 In-stock
25 mg USD 290 In-stock
50 mg   Get quote  
100 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Kemptide:

Kemptide Related Antibodies

Top Publications Citing Use of Products

View All PKA Isoform Specific Products:

View All Isoforms
PKA PKAR

  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

Kemptide is a synthetic heptapeptide that acts as a specific substrate for cAMP-dependent protein kinase (PKA).

In Vitro

Kemptide is a synthetic construct of a substrate for cAMP-dependent protein kinase (PK). Different types of intact cells catalyze the phosphorylation of Kemptide in the presence of extracellular ATP and cAMP with Km values of 3-4 uM for Kemptide[1]. Kemptide is a synthetic peptide substrate derived from pyruvate kinase[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Kemptide Related Antibodies
Molecular Weight

771.91

Formula

C32H61N13O9
CAS No.
Appearance

Solid

Color

White to off-white

Sequence

Leu-Arg-Arg-Ala-Ser-Leu-Gly
Sequence Shortening

LRRASLG
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : 33.3 mg/mL (43.14 mM; Need ultrasonic and warming)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.2955 mL 6.4774 mL 12.9549 mL
5 mM 0.2591 mL 1.2955 mL 2.5910 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (129.55 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References
Kinase Assay
[2]

PKA activity is measured in pools of 10 oocytes at various stages of maturation using the SignaTECT cAMP-Dependent Protein Kinase (PKA) Assay System. Measurements of basal PKA activity are performed in the absence of exogenous cAMP, whereas measurements of total (cAMP-stimulated) PKA activity are performed in reaction buffer containing 0.025 mM cAMP[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 1.2955 mL 6.4774 mL 12.9549 mL 32.3872 mL
5 mM 0.2591 mL 1.2955 mL 2.5910 mL 6.4774 mL
10 mM 0.1295 mL 0.6477 mL 1.2955 mL 3.2387 mL
15 mM 0.0864 mL 0.4318 mL 0.8637 mL 2.1591 mL
20 mM 0.0648 mL 0.3239 mL 0.6477 mL 1.6194 mL
25 mM 0.0518 mL 0.2591 mL 0.5182 mL 1.2955 mL
30 mM 0.0432 mL 0.2159 mL 0.4318 mL 1.0796 mL
40 mM 0.0324 mL 0.1619 mL 0.3239 mL 0.8097 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

Kemptide Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Kemptide65189-71-1PKAProtein kinase AInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Kemptide
Cat. No.:
HY-P0248
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

Request for HNMR Report

We have received your request and will respond to you as soon as possible.