LTV-1

Cat. No.: HY-18667 Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

LTV-1 is a potent lymphoid tyrosine phosphatase (LYP) inhibitor in T cells with an IC₅₀ of 508 nM. LTV-1 has the potential for autoimmunity treatment.

For research use only. We do not sell to patients.

LTV-1 Chemical Structure

CAS No. : 347379-29-7

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 220	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 220	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 200	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 320	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 1100	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 1680	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

LTV-1 is a potent lymphoid tyrosine phosphatase (LYP) inhibitor in T cells with an IC₅₀ of 508 nM. LTV-1 has the potential for autoimmunity treatment^[1].

IC₅₀ & Target

IC50: 508 nM (LYP, in T cells)^[1]

In Vitro

LTV-1 (1.25-40 μM) has no notable effect on cell viability (metabolic rate assay), even at the highest concentration tested (40 μM). Thus, LTV-1 is not cytotoxic in HeLa cells, Jurkat TAg T cells and peripheral blood mononuclear cells (PBMC)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	HeLa cells, Jurkat TAg T cells, and PBMC
Concentration:	1.25, 2.5, 5.0, 10, 20 , and 40 μM
Incubation Time:	48 hours
Result:	Had no notable effect on cell viability (metabolic rate assay), even at the highest concentration tested (40 μM).

Molecular Weight

472.51

Formula

C₂₆H₂₀N₂O₅S

CAS No.

347379-29-7

Appearance

Solid

Color

Yellow to brown

SMILES

O=C(O)C1=CC=CC(COC2=CC=C(/C=C(C(N3C4=CC=CC=C4C)=O)\C(NC3=S)=O)C=C2)=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 22.73 mg/mL (48.10 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.1164 mL	10.5818 mL	21.1636 mL
5 mM	0.4233 mL	2.1164 mL	4.2327 mL
10 mM	0.2116 mL	1.0582 mL	2.1164 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.29 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.08 mg/mL (4.40 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.08 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (271 KB) SDS (252 KB)

COA (246 KB) HNMR (199 KB)

Handling Instructions (2659 KB)

References

[1]. Vang T, et al. LYP inhibits T-cell activation when dissociated from CSK. Nat Chem Biol. 2012 Mar 18;8(5):437-446. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.1164 mL	10.5818 mL	21.1636 mL	52.9089 mL
	5 mM	0.4233 mL	2.1164 mL	4.2327 mL	10.5818 mL
	10 mM	0.2116 mL	1.0582 mL	2.1164 mL	5.2909 mL
	15 mM	0.1411 mL	0.7055 mL	1.4109 mL	3.5273 mL
	20 mM	0.1058 mL	0.5291 mL	1.0582 mL	2.6454 mL
	25 mM	0.0847 mL	0.4233 mL	0.8465 mL	2.1164 mL
	30 mM	0.0705 mL	0.3527 mL	0.7055 mL	1.7636 mL
	40 mM	0.0529 mL	0.2645 mL	0.5291 mL	1.3227 mL

LTV-1 Related Classifications

Inflammation/Immunology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

LTV-1347379-29-7LTV1LTV 1PhosphataselymphoidtyrosinephosphataseLYPautoimmunityInhibitorinhibitorinhibit

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