Lin28-let-7a antagonist 1

Name: Lin28-let-7a antagonist 1
Brand: MedChemExpress
SKU: HY-100692
Rating: 5.0 (2 reviews)

Cat. No.: HY-100692 Purity: 99.48%: FAQs
Data Sheet SDS COA Handling Instructions

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Lin28-let-7a antagonist 1 shows a clear antagonistic effect against the Lin28-let-7a interaction with an IC₅₀ of 4.03 μM for Lin28A-let-7a-1 interaction.

For research use only. We do not sell to patients.

Lin28-let-7a antagonist 1 Chemical Structure

CAS No. : 2024548-03-4

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 359	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 359	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 280	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 420	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 756	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 1134	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 1700	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 2 publication(s) in Google Scholar

2 Publications Citing Use of MCE Lin28-let-7a antagonist 1

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Purity & Documentation
References
Customer Review

Description

Lin28-let-7a antagonist 1 shows a clear antagonistic effect against the Lin28-let-7a interaction with an IC₅₀ of 4.03 μM for Lin28A-let-7a-1 interaction.

IC₅₀ & Target

IC50：4.03 μM (Lin28A-let-7a-1)^[1].

In Vitro

Lin28-let-7a antagonist 1 (compound 1) shows a clear antagonistic effect against the Lin28A-let-7a-1 interaction with an IC₅₀ of 4.03 μM. Interestingly, Lin28-let-7a antagonist 1 also inhibits the Lin28B-let-7 interaction, with slightly reduced potency. Dicer processing assay reveals that Lin28-let-7a antagonist 1 clearly blocks the formation of the Lin28A-pre-let-7g complex and induces miRNA processing, resulting in the generation of mature let-7g miRNA. When the expression of both Lin28A and Lin28B are knocked-down using siRNA in JAR cells, the Lin28-let-7a antagonist 1-induced increase in cellular let-7 levels is clearly attenuated. Lin28A-let-7a-IN-1 increases mature let-7 levels in PA-1 cells that express high level of Lin28A. On the other hand, let-7 levels are not affected by 1 in MCF7 cells that rarely express Lin28 proteins. Taken together, Lin28-let-7a antagonist 1 specifically induces the increase in the cellular let-7 levels by targeting Lin28^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

583.59

Formula

C₃₁H₂₉N₅O₇

CAS No.

2024548-03-4

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(O)C(C=C1)=CC=C1N2N=CC3=C2C4=CC([N+]([O-])=O)=C(N5CCN(C(OCC6=CC=CC=C6)=O)CC5)C=C4OC3(C)C

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (171.35 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.7135 mL	8.5677 mL	17.1353 mL
5 mM	0.3427 mL	1.7135 mL	3.4271 mL
10 mM	0.1714 mL	0.8568 mL	1.7135 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

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Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.48%

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Data Sheet (278 KB) SDS (251 KB)

COA (257 KB) HNMR (198 KB) LCMS (111 KB)

Handling Instructions (2659 KB)

References

[1]. Lim D, et al. Discovery of a Small-Molecule Inhibitor of Protein-MicroRNA Interaction Using Binding Assay with a Site-Specifically Labeled Lin28. J Am Chem Soc. 2016 Oct 7. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.7135 mL	8.5677 mL	17.1353 mL	42.8383 mL
	5 mM	0.3427 mL	1.7135 mL	3.4271 mL	8.5677 mL
	10 mM	0.1714 mL	0.8568 mL	1.7135 mL	4.2838 mL
	15 mM	0.1142 mL	0.5712 mL	1.1424 mL	2.8559 mL
	20 mM	0.0857 mL	0.4284 mL	0.8568 mL	2.1419 mL
	25 mM	0.0685 mL	0.3427 mL	0.6854 mL	1.7135 mL
	30 mM	0.0571 mL	0.2856 mL	0.5712 mL	1.4279 mL
	40 mM	0.0428 mL	0.2142 mL	0.4284 mL	1.0710 mL
	50 mM	0.0343 mL	0.1714 mL	0.3427 mL	0.8568 mL
	60 mM	0.0286 mL	0.1428 mL	0.2856 mL	0.7140 mL
	80 mM	0.0214 mL	0.1071 mL	0.2142 mL	0.5355 mL
	100 mM	0.0171 mL	0.0857 mL	0.1714 mL	0.4284 mL