2. Apoptosis
  3. Bcl-2 Family
  4. MIK665

MIK665 (S-64315), derived from S63845, is a myeloid cell leukemia sequence 1 (MCL1) inhibitor. MIK665 has an IC50 of 1.81 nM for MCL1.

For research use only. We do not sell to patients.

MIK665 Chemical Structure

MIK665 Chemical Structure

CAS No. : 1799631-75-6

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 530 In-stock
Solution
10 mM * 1 mL in DMSO USD 530 In-stock
Solid
1 mg USD 166 In-stock
5 mg USD 350 In-stock
10 mg USD 550 In-stock
50 mg USD 1650 In-stock
100 mg   Get quote  
200 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 14 publication(s) in Google Scholar

Other Forms of MIK665:

MIK665 Related Antibodies

Top Publications Citing Use of Products

    MIK665 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2020 Jun 8;11(6):443.  [Abstract]

    S64315+A-1331852 and S64315+ABT-263 significantly reduces cell viability compared with DMSO or with single drug treated conditions in all melanoma cell lines at sub-micromolar doses.

    MIK665 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2020 Jun 8;11(6):443.  [Abstract]

    S64315+A-1331852 and S64315+ABT-263 significantly reduces cell viability compared with DMSO or with single drug treated conditions in all melanoma cell lines at sub-micromolar doses.

    View All Bcl-2 Family Isoform Specific Products:

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    Bcl-2 Bcl-xL Bcl-W Mcl-1 Bfl-1 Bcl-B Bax Bak Bim

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    MIK665 (S-64315), derived from S63845, is a myeloid cell leukemia sequence 1 (MCL1) inhibitor[1]. MIK665 has an IC50 of 1.81 nM for MCL1[2].

    IC50 & Target[1]

    Mcl-1

    1.81 nM (IC50)

    In Vitro

    MIK665 (S-64315) has similar synergistic effects as S63845, when combined with ABT-199. The combination S64315 (0.156-10 μM) and ABT-199 (625 nM) has similar efficacy in reducing the cell viability of representative melanoma lines (MB2141, MB3616, MB3961, MB4667, A375, and 1205Lu cells)[1].
    ? MIK665 is extracted from patent WO2016207225A1, compound Preparation 13, and inhibits H929 cell with an IC50 of 250 nM[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    MIK665 Related Antibodies
    Clinical Trial
    Molecular Weight

    875.41

    Formula

    C47H44ClFN6O6S
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    OC([C@@H](CC(C=CC=C1)=C1OCC2=CC=NC(C3=CC=CC=C3OC)=N2)OC4=C5[C@]([C@]6=C(C)C(Cl)=C(OCCN7CCN(C)CC7)C=C6)=C(C8=CC=C(F)C=C8)SC5=NC=N4)=O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 125 mg/mL (142.79 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.1423 mL 5.7116 mL 11.4232 mL
    5 mM 0.2285 mL 1.1423 mL 2.2846 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

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    Volume (start)

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    C2

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    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (2.38 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 2.08 mg/mL (2.38 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.11%

    COA (254 KB) LCMS (96 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.1423 mL 5.7116 mL 11.4232 mL 28.5580 mL
    5 mM 0.2285 mL 1.1423 mL 2.2846 mL 5.7116 mL
    10 mM 0.1142 mL 0.5712 mL 1.1423 mL 2.8558 mL
    15 mM 0.0762 mL 0.3808 mL 0.7615 mL 1.9039 mL
    20 mM 0.0571 mL 0.2856 mL 0.5712 mL 1.4279 mL
    25 mM 0.0457 mL 0.2285 mL 0.4569 mL 1.1423 mL
    30 mM 0.0381 mL 0.1904 mL 0.3808 mL 0.9519 mL
    40 mM 0.0286 mL 0.1428 mL 0.2856 mL 0.7140 mL
    50 mM 0.0228 mL 0.1142 mL 0.2285 mL 0.5712 mL
    60 mM 0.0190 mL 0.0952 mL 0.1904 mL 0.4760 mL
    80 mM 0.0143 mL 0.0714 mL 0.1428 mL 0.3570 mL
    100 mM 0.0114 mL 0.0571 mL 0.1142 mL 0.2856 mL
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    MIK665 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    MIK6651799631-75-6S-64315MIK 665MIK-665S64315S 64315Bcl-2 FamilyBH3mimeticmyeloidcellsleukemiadeathmelanomaInhibitorinhibitorinhibit

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