Mephenesin

Cat. No.: HY-B1283 Purity: 99.69%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Mephenesin is an NMDA receptor antagonist. Mephenesin is also a central muscle relaxant with antianxiety, muscle-paralyzing and anticonvulsant effects. Mephenesin acts directly on the skeletal muscle fibres to produce skeletal muscle relaxation. Mephenesin is promising for research of spasticity or as an adjunctive treatment of painful muscle spasm.

For research use only. We do not sell to patients.

Mephenesin Chemical Structure

CAS No. : 59-47-2

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 28	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 28	In-stock	Estimated Time of Arrival: December 31
Solid
500 mg	USD 25	In-stock	Estimated Time of Arrival: December 31
1 g	USD 40	In-stock	Estimated Time of Arrival: December 31
5 g		Get quote
10 g		Get quote

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Other Forms of Mephenesin:

Mephenesin (Standard) Get quote

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AMPA Receptor NMDA Receptor Kainate Receptor

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

NMDA Receptor

In Vivo

Mephenesin (5-155 mg/kg, i.v., 10 min intervals and measurements were taken 4 min after administration) elicits polysynaptic flexor reflexes and monosynaptic knee-jerk reflexes and reduces the polysynaptic component of the ventral root potential in cats^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Adult cats^[3]
Dosage:	5-155 mg/kg
Administration:	i.v., 10 min intervals and measurements were taken 4 min after administration
Result:	Produced a 95% reduction in the flexor contractions and reduced the areas of the polysynaptic components of the ventral root potentials to approximately half the control values in cats.

Molecular Weight

182.22

Formula

C₁₀H₁₄O₃

CAS No.

59-47-2

Appearance

Solid

Color

White to off-white

SMILES

OCC(O)COC1=CC=CC=C1C

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL (548.79 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.4879 mL	27.4394 mL	54.8787 mL
5 mM	1.0976 mL	5.4879 mL	10.9757 mL
10 mM	0.5488 mL	2.7439 mL	5.4879 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (13.72 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (13.72 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (13.72 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.69%

Select Batch:

Data Sheet (272 KB) SDS (392 KB)

COA (245 KB) RP-HPLC (250 KB)

Handling Instructions (2659 KB)

References

[1]. Mephenesin: abuse and dependence. Prescrire Int. 2013 May;22(138):127-128. [Content Brief]

[2]. ?cija P, et al. Conformational flexibility of mephenesin. J Phys Chem B. 2014 May 22;118(20):5357-5364. [Content Brief]

[3]. Crankshaw DP, et al. Mephenesin, methocarbamol, chlordiazepoxide and diazepam: actions on spinal reflexes and ventral root potentials. Br J Pharmacol. 1970 Jan;38(1):148-56. [Content Brief]

[4]. Ludwig B J, et al. Muscle-paralyzing compounds related to mephenesin[J]. Journal of the American Chemical Society, 1952, 74(8): 1935-1939.

[5]. DIAZ-RIVERA RS, et al. Muscular relaxation in tetanus with special reference to the effect of mephenesin. Ann Intern Med. 1954 Mar;40(3):563-80. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	5.4879 mL	27.4394 mL	54.8787 mL	137.1968 mL
	5 mM	1.0976 mL	5.4879 mL	10.9757 mL	27.4394 mL
	10 mM	0.5488 mL	2.7439 mL	5.4879 mL	13.7197 mL
	15 mM	0.3659 mL	1.8293 mL	3.6586 mL	9.1465 mL
	20 mM	0.2744 mL	1.3720 mL	2.7439 mL	6.8598 mL
	25 mM	0.2195 mL	1.0976 mL	2.1951 mL	5.4879 mL
	30 mM	0.1829 mL	0.9146 mL	1.8293 mL	4.5732 mL
	40 mM	0.1372 mL	0.6860 mL	1.3720 mL	3.4299 mL
	50 mM	0.1098 mL	0.5488 mL	1.0976 mL	2.7439 mL
	60 mM	0.0915 mL	0.4573 mL	0.9146 mL	2.2866 mL
	80 mM	0.0686 mL	0.3430 mL	0.6860 mL	1.7150 mL
	100 mM	0.0549 mL	0.2744 mL	0.5488 mL	1.3720 mL

Mephenesin Related Classifications

Neurological Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Mephenesin59-47-2iGluRIonotropic glutamate receptorsNMDA receptorentral muscle relaxantantianxietymuscleparalyzinganticonvulsantskeletal muscle fibresInhibitorinhibitorinhibit

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