Metoclopramide hydrochloride hydrate

Name: Metoclopramide hydrochloride hydrate
Brand: MedChemExpress
SKU: HY-17382A
Rating: 5.0 (2 reviews)

Cat. No.: HY-17382A Purity: 99.96%: FAQs
Data Sheet SDS COA Handling Instructions

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Metoclopramide hydrochloride hydrate is a potent antagonist of 5-HT₃ and dopamine D2 receptor, with IC₅₀s of 308 nM and 483 nM, respectively. Metoclopramide hydrochloride hydrate can be used for the research of nausea and vomiting, gastro-oesophageal reflux, and gastroparesis.

For research use only. We do not sell to patients.

Metoclopramide hydrochloride hydrate Chemical Structure

CAS No. : 54143-57-6

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	Get quote
5 g	Get quote

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Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of Metoclopramide hydrochloride hydrate:

Metoclopramide In-stock
Metoclopramide hydrochloride Get quote
Metoclopramide (hydrochloride hydrate) (Standard) Get quote

2 Publications Citing Use of MCE Metoclopramide hydrochloride hydrate

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Dopamine Receptor D₁ Receptor D₂ Receptor D₃ Receptor D₄ Receptor D₅ Receptor

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]

5-HT₃ Receptor

308 nM (IC₅₀)

D₂ Receptor

483 nM (IC₅₀)

In Vitro

Metoclopramide (0.01-10 μM) hydrochloride hydrate stimulates aldosterone release in isolated perfused rat zona glomerulosa cells^[3].
Metoclopramide hydrochloride hydrate results in prokinesis by four mechanisms: inhibition of presynaptic D2 receptors and stimulation of presynaptic excitatory 5-HT4 receptors, thereby allowing acetylcholine (ACh) release from intrinsic cholinergic motor neurons; inhibition of D2 postsynaptic receptors; and antagonism of the presynaptic inhibition of muscarinic receptors, leading to further augmentation of ACh release^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Metoclopramide (6.7 μg/g; s.c. daily for 50 days) hydrochloride hydrate significantly increases the number and the volume of lactotroph cells of the pituitary gland during all phases of the estrous cycle^[4].
Metoclopramide (5-40 mg/kg; i.p.) hydrochloride hydrate induces catalepsy and antagonizes Apomorphine induced cage climbing behaviour in mice^[5].
Metoclopramide (1.25-2.5 mg/kg; i.p.) hydrochloride hydrate induces stereotyped cage climbing behaviour in mice^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Adult, virgin female mice of the Swiss EPM-1 strain^[4]
Dosage:	6.7 µg/g
Administration:	S.c. daily for 50 days
Result:	Increased the number but also stimulated the metabolic activity of lactotrophs.

Molecular Weight

354.27

Formula

C₁₄H₂₅Cl₂N₃O₃

CAS No.

54143-57-6

Appearance

Solid

Color

White to off-white

SMILES

O=C(NCCN(CC)CC)C1=CC(Cl)=C(N)C=C1OC.[H]Cl.[H]O[H]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (141.14 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.8227 mL	14.1135 mL	28.2271 mL
5 mM	0.5645 mL	2.8227 mL	5.6454 mL
10 mM	0.2823 mL	1.4114 mL	2.8227 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.06 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (7.06 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.96%

Select Batch:

Data Sheet (276 KB) SDS (392 KB)

COA (248 KB) LCMS (93 KB)

Handling Instructions (2659 KB)

References

[1]. Hirokawa Y, et, al. Synthesis and structure-activity relationships of 4-amino-5-chloro-N-(1,4-dialkylhexahydro-1,4-diazepin-6-yl)-2-methoxybenzamide derivatives, novel and potent serotonin 5-HT3 and dopamine D2 receptors dual antagonist. Chem Pharm Bull (Tokyo). 2002 Jul;50(7):941-59. [Content Brief]

[2]. Rao AS, et, al. Review article: metoclopramide and tardive dyskinesia. Aliment Pharmacol Ther. 2010 Jan;31(1):11-9. [Content Brief]

[3]. Edwards CR, et, al. In vivo and in vitro studies on the effect of metoclopramide on aldosterone secretion. Clin Endocrinol (Oxf). 1980 Jul;13(1):45-50. [Content Brief]

[4]. Bhosale KB, et, al. Dose-dependent response of central dopaminergic systems to metoclopramide in mice. Indian J Exp Biol. 1997 Jun;35(6):618-22. [Content Brief]

[5]. Gomes RCT, et, al. Effects of metoclopramide on the mouse anterior pituitary during the estrous cycle. Clinics (Sao Paulo). 2011;66(6):1101-4. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.8227 mL	14.1135 mL	28.2271 mL	70.5676 mL
	5 mM	0.5645 mL	2.8227 mL	5.6454 mL	14.1135 mL
	10 mM	0.2823 mL	1.4114 mL	2.8227 mL	7.0568 mL
	15 mM	0.1882 mL	0.9409 mL	1.8818 mL	4.7045 mL
	20 mM	0.1411 mL	0.7057 mL	1.4114 mL	3.5284 mL
	25 mM	0.1129 mL	0.5645 mL	1.1291 mL	2.8227 mL
	30 mM	0.0941 mL	0.4705 mL	0.9409 mL	2.3523 mL
	40 mM	0.0706 mL	0.3528 mL	0.7057 mL	1.7642 mL
	50 mM	0.0565 mL	0.2823 mL	0.5645 mL	1.4114 mL
	60 mM	0.0470 mL	0.2352 mL	0.4705 mL	1.1761 mL
	80 mM	0.0353 mL	0.1764 mL	0.3528 mL	0.8821 mL
	100 mM	0.0282 mL	0.1411 mL	0.2823 mL	0.7057 mL