2. Membrane Transporter/Ion Channel Autophagy
  3. Potassium Channel Autophagy
  4. NS1643

NS1643 is a partial agonist of human ether-a-go-go-related gene (hERG) K+ channels with an EC50 of 10.5 μM. NS1643 inhibits the growth of breast cancer tumors in TNBC mouse models. NS1643 inhibits cell migration and invasion of breast cancer cells.

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NS1643 Chemical Structure

NS1643 Chemical Structure

CAS No. : 448895-37-2

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Based on 1 publication(s) in Google Scholar

NS1643 Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE NS1643

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

NS1643 is a partial agonist of human ether-a-go-go-related gene (hERG) K+ channels with an EC50 of 10.5 μM. NS1643 inhibits the growth of breast cancer tumors in TNBC mouse models. NS1643 inhibits cell migration and invasion of breast cancer cells[1][2].

IC50 & Target

EC50: 10.5 μM (hERG) K+ channel[1]
In Vitro

NS1643 (0-100 μM) dose- and voltage-dependently increases hERG current in oocytes[1].
NS1643 (3, 10 and 30 μM) slows the rate of hERG inactivation throughout the testing voltage range and reduces the extent of hERG channel rectification[1].
NS1643 (10 and 50 μM) dose-dependently inhibits cell migration and invasion of MDA-MB-231 and SKBR3 cancer cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

NS1643 Related Antibodies

Western Blot Analysis[2]

Cell Line: MDA-MB-231, SKBR3 and MCF7 breast cancer cell lines
Concentration: 50 μM
Incubation Time: 24 h
Result: Decreased Vimentin, N-cadherin and CD44 levels, and increased E-cadherin in breast cancer cell lines.
In Vivo

NS1643 (6 mg/kg; i.p., twice per week) inhibits breast tumor metastasis[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: NSG mice with human-derived TNBC tumor xenografts[2]
Dosage: 6 mg/kg
Administration: Intraperitoneal injection; 6 mg/kg; twice per week
Result: Significantly reduceed tumor growth and the metastatic liver tumors were significantly smaller than those in the control group. Decreased levels of human nuclear antigen (HNA).
Molecular Weight

380.24

Formula

C15H10F6N2O3
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(NC1=C(O)C=CC(C(F)(F)F)=C1)NC2=C(O)C=CC(C(F)(F)F)=C2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (262.99 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6299 mL 13.1496 mL 26.2992 mL
5 mM 0.5260 mL 2.6299 mL 5.2598 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (6.57 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (6.57 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.14%

COA (252 KB) HNMR (220 KB) RP-HPLC (264 KB) LCMS (104 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.6299 mL 13.1496 mL 26.2992 mL 65.7480 mL
5 mM 0.5260 mL 2.6299 mL 5.2598 mL 13.1496 mL
10 mM 0.2630 mL 1.3150 mL 2.6299 mL 6.5748 mL
15 mM 0.1753 mL 0.8766 mL 1.7533 mL 4.3832 mL
20 mM 0.1315 mL 0.6575 mL 1.3150 mL 3.2874 mL
25 mM 0.1052 mL 0.5260 mL 1.0520 mL 2.6299 mL
30 mM 0.0877 mL 0.4383 mL 0.8766 mL 2.1916 mL
40 mM 0.0657 mL 0.3287 mL 0.6575 mL 1.6437 mL
50 mM 0.0526 mL 0.2630 mL 0.5260 mL 1.3150 mL
60 mM 0.0438 mL 0.2192 mL 0.4383 mL 1.0958 mL
80 mM 0.0329 mL 0.1644 mL 0.3287 mL 0.8218 mL
100 mM 0.0263 mL 0.1315 mL 0.2630 mL 0.6575 mL
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NS1643 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

NS1643448895-37-2NS 1643NS-1643Potassium ChannelAutophagyKcsATNBCoocytemigrationinvasionMDA-MB-231SKBR3MCF7Inhibitorinhibitorinhibit

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