2. Stem Cell/Wnt
  3. Oct3/4
  4. O4I2

O4I2 is a potent Oct3/4 inducer. O4I2 induces the expression of pluripotent-associated genes Lin28, Sox2 and Nanog, and suppresses Rex1.

For research use only. We do not sell to patients.

O4I2 Chemical Structure

O4I2 Chemical Structure

CAS No. : 165682-93-9

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 66 In-stock
Solution
10 mM * 1 mL in DMSO USD 66 In-stock
Solid
1 mg USD 30 In-stock
5 mg USD 60 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

O4I2 Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

O4I2 is a potent Oct3/4 inducer. O4I2 induces the expression of pluripotent-associated genes Lin28, Sox2 and Nanog, and suppresses Rex1[1].

In Vitro

O4I2 not only promotes Oct3/4 expression by activating its promoter site, but also stabilize the Oct3/4 protein in HEK293 cells[1].
O4I2 markedly stimulates Oct3/4 even at 5 μM after 72 hrs treatment on the translational level[1].
O4I2 (20 μM) accumulates Oct3/4 in NCCIT occurred already 2 hrs after incubation, while the detectable Sox2 increase appears much later, implicating compound-mediated activation of Oct3/4 might be different between embryonic kidney HEK293 cells and embryonal carcinoma NCCIT cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

O4I2 Related Antibodies

Immunofluorescence[1]

Cell Line: HF (human fibroblasts) cells.
Concentration: 5-20 µM.
Incubation Time: 5 days.
Result: Successfully detected co-expression of Oct3/4 and Sox2, as well as Nanog and Klf4.
Molecular Weight

282.75

Formula

C12H11ClN2O2S
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(C1=CSC(NC2=CC=C(Cl)C=C2)=N1)OCC
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : ≥ 42 mg/mL (148.54 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.5367 mL 17.6835 mL 35.3669 mL
5 mM 0.7073 mL 3.5367 mL 7.0734 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 2.5 mg/mL (8.84 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.5367 mL 17.6835 mL 35.3669 mL 88.4173 mL
5 mM 0.7073 mL 3.5367 mL 7.0734 mL 17.6835 mL
10 mM 0.3537 mL 1.7683 mL 3.5367 mL 8.8417 mL
15 mM 0.2358 mL 1.1789 mL 2.3578 mL 5.8945 mL
20 mM 0.1768 mL 0.8842 mL 1.7683 mL 4.4209 mL
25 mM 0.1415 mL 0.7073 mL 1.4147 mL 3.5367 mL
30 mM 0.1179 mL 0.5894 mL 1.1789 mL 2.9472 mL
40 mM 0.0884 mL 0.4421 mL 0.8842 mL 2.2104 mL
50 mM 0.0707 mL 0.3537 mL 0.7073 mL 1.7683 mL
60 mM 0.0589 mL 0.2947 mL 0.5894 mL 1.4736 mL
80 mM 0.0442 mL 0.2210 mL 0.4421 mL 1.1052 mL
100 mM 0.0354 mL 0.1768 mL 0.3537 mL 0.8842 mL
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O4I2 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

O4I2165682-93-9Oct3/4POU5F1Slc22a3iPSCsOct3Oct4activatorstemcellpluripotentnanogsox2lin28Rex1Inhibitorinhibitorinhibit

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