Nesolicaftor (Synonyms: PTI-428)

Name: Nesolicaftor
Brand: MedChemExpress
SKU: HY-111680
Rating: 5.0 (2 reviews)

Cat. No.: HY-111680 Purity: 99.37%: FAQs
Data Sheet SDS COA Handling Instructions

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Nesolicaftor (PTI-428) is a specific cystic fibrosis transmembrane conductance regulator (CFTR) amplifier.

For research use only. We do not sell to patients.

Nesolicaftor Chemical Structure

CAS No. : 1953130-87-4

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 302	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 302	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	USD 130	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 275	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 495	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 1320	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 1870	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 2 publication(s) in Google Scholar

2 Publications Citing Use of MCE Nesolicaftor

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Description

Nesolicaftor (PTI-428) is a specific cystic fibrosis transmembrane conductance regulator (CFTR) amplifier^[1].

IC₅₀ & Target

CFTR^[1]

In Vitro

Nesolicaftor (PTI-428) (30 μM, 24 h) can increase CFTR function, such as causing a larger change in inh-172 in 16HBE14o-cells expressing G542X-CFTR, also can increase in F508del-CFTR function of CFBE41o-cells expressing F508del-CFTR in a dose-dependent manner^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

354.36

Formula

C₁₈H₁₈N₄O₄

CAS No.

1953130-87-4

Appearance

Solid

Color

White to off-white

SMILES

O=C(C1=CC(C2=CC=CC=C2)=NO1)N[C@H]3C[C@H](C4=NN=C([C@H](O)C)O4)C3

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 250 mg/mL (705.50 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.8220 mL	14.1099 mL	28.2199 mL
5 mM	0.5644 mL	2.8220 mL	5.6440 mL
10 mM	0.2822 mL	1.4110 mL	2.8220 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (5.87 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (5.87 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (5.87 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.37%

Select Batch:

Data Sheet (273 KB) SDS (252 KB)

COA (248 KB) LCMS (94 KB)

Handling Instructions (2659 KB)

References

[1]. Mijnders M, et al. Correcting CFTR folding defects by small-molecule correctors to cure cystic fibrosis. Curr Opin Pharmacol. 2017 Jun;34:83-90. [Content Brief]

[2]. Arianna Venturini, et al. Comprehensive Analysis of Combinatorial Pharmacological Treatments to Correct Nonsense Mutations in the CFTR Gene. Int J Mol Sci. 2021 Nov 4;22(21):11972. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.8220 mL	14.1099 mL	28.2199 mL	70.5497 mL
	5 mM	0.5644 mL	2.8220 mL	5.6440 mL	14.1099 mL
	10 mM	0.2822 mL	1.4110 mL	2.8220 mL	7.0550 mL
	15 mM	0.1881 mL	0.9407 mL	1.8813 mL	4.7033 mL
	20 mM	0.1411 mL	0.7055 mL	1.4110 mL	3.5275 mL
	25 mM	0.1129 mL	0.5644 mL	1.1288 mL	2.8220 mL
	30 mM	0.0941 mL	0.4703 mL	0.9407 mL	2.3517 mL
	40 mM	0.0705 mL	0.3527 mL	0.7055 mL	1.7637 mL
	50 mM	0.0564 mL	0.2822 mL	0.5644 mL	1.4110 mL
	60 mM	0.0470 mL	0.2352 mL	0.4703 mL	1.1758 mL
	80 mM	0.0353 mL	0.1764 mL	0.3527 mL	0.8819 mL
	100 mM	0.0282 mL	0.1411 mL	0.2822 mL	0.7055 mL

Nesolicaftor Related Classifications

Endocrinology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Nesolicaftor1953130-87-4PTI-428PTI428PTI 428CFTRAutophagyCystic fibrosis transmembrane conductance regulatorcystic fibrosisInhibitorinhibitorinhibit

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Nesolicaftor (Synonyms: PTI-428)

Customer Review

Nesolicaftor Related Antibodies

2 Publications Citing Use of MCE Nesolicaftor

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

Nesolicaftor Related Antibodies

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Complete Stock Solution Preparation Table

Nesolicaftor Related Classifications

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Your Recently Viewed Products:

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