Parbendazole (Synonyms: SKF 29044)

Name: Parbendazole
Brand: MedChemExpress
SKU: HY-115364
Rating: 5.0 (6 reviews)

Cat. No.: HY-115364 Purity: 99.87%: FAQs
Data Sheet SDS COA Handling Instructions

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Parbendazole is a potent inhibitor of microtubule assembly, destabilizes tubulin, with an EC₅₀ of 530?nM, and exhibits a broad-spectrum anthelmintic activity.

For research use only. We do not sell to patients.

Parbendazole Chemical Structure

CAS No. : 14255-87-9

Size	Price	Stock	Quantity

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 61	In-stock	Estimated Time of Arrival: December 31
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10 mM * 1 mL in DMSO	USD 61	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 35	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 55	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 88	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 132	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 209	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 6 publication(s) in Google Scholar

Other Forms of Parbendazole:

Parbendazole-d₃ Get quote
Parbendazole (Standard) Get quote

6 Publications Citing Use of MCE Parbendazole

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Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

Parbendazole is a potent inhibitor of microtubule assembly, destabilizes tubulin, with an EC₅₀ of 530?nM, and exhibits a broad-spectrum anthelmintic activity.

IC₅₀ & Target

EC50: 530 nM (tubulin)^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
CAL-27	IC₅₀	173 nM Compound: Parbendazole	Antiproliferative activity against human CAL-27 cells assessed as cell growth inhibition incubated for 72 hrs by CCK-8 assay Antiproliferative activity against human CAL-27 cells assessed as cell growth inhibition incubated for 72 hrs by CCK-8 assay	[PMID: 35576703]
FaDu	IC₅₀	160 nM Compound: Parbendazole	Antiproliferative activity against human FaDu cells assessed as cell growth inhibition incubated for 72 hrs by CCK-8 assay Antiproliferative activity against human FaDu cells assessed as cell growth inhibition incubated for 72 hrs by CCK-8 assay	[PMID: 35576703]
HFF-1	IC₅₀	> 20 μM Compound: Parbendazole	Cytotoxicity against human HFF-1 clls assessed as reduction in cell viability incubated for 72 hrs by CCK-8 assay Cytotoxicity against human HFF-1 clls assessed as reduction in cell viability incubated for 72 hrs by CCK-8 assay	[PMID: 35576703]
HN-6	IC₅₀	102 nM Compound: Parbendazole	Antiproliferative activity against human HN-6 cells assessed as cell growth inhibition incubated for 72 hrs by CCK-8 assay Antiproliferative activity against human HN-6 cells assessed as cell growth inhibition incubated for 72 hrs by CCK-8 assay	[PMID: 35576703]

In Vitro

Parbendazole is a tubulin destabilizer, with an EC₅₀ of 530?nM, and can induce DNA damage^[1]. Parbendazole (2-10 μM) inhibits the assembly of microtubules dose-dependently, with an IC₅₀ of 3 μM. Parbendazole (2-20 μM)-treated cells show an complete absence of microtubules in Vero cells^[2]. Parbendazole (up to 10 μM) inhibits the growth of CLd-AXE myxamoebae. Parbendazole (2-5 μM) potently inhibits tubulin purified from the wild-type myxamoebae^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

247.29

Formula

C₁₃H₁₇N₃O₂

CAS No.

14255-87-9

Appearance

Solid

Color

White to light yellow

SMILES

O=C(OC)NC1=NC2=CC=C(CCCC)C=C2N1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 4 mg/mL (16.18 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.0438 mL	20.2192 mL	40.4384 mL
5 mM	0.8088 mL	4.0438 mL	8.0877 mL
10 mM	0.4044 mL	2.0219 mL	4.0438 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 0.4 mg/mL (1.62 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 0.4 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (4.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 0.4 mg/mL (1.62 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 0.4 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (4.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 0.4 mg/mL (1.62 mM); Clear solution

This protocol yields a clear solution of ≥ 0.4 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (4.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.87%

Select Batch:

Data Sheet (274 KB) SDS (418 KB)

COA (244 KB) LCMS (268 KB)

Handling Instructions (2659 KB)

References

[1]. Lo YC, et al. Computational Cell Cycle Profiling of Cancer Cells for Prioritizing FDA-Approved Drugs with Repurposing Potential. Sci Rep. 2017 Sep 12;7(1):11261. [Content Brief]

[2]. Havercroft JC, et al. Binding of parbendazole to tubulin and its influence on microtubules in tissue-culture cells as revealed by immunofluorescence microscopy. J Cell Sci. 1981 Jun;49:195-204. [Content Brief]

[3]. Foster KE, et al. A mutant beta-tubulin confers resistance to the action of benzimidazole-carbamate microtubule inhibitors both in vivo and in vitro. Eur J Biochem. 1987 Mar 16;163(3):449-55. [Content Brief]

Kinase Assay ^[2]	Pure tubulin is obtained from sheep brain by 2 cycles of assembly and disassembly in vitro. Immediately prior to use the protein is centrifuged at 130000 g for 30 min to remove any aggregates. It is used at a protein concentration of 0-2 mg/mL in 0.025 M Pipes buffer, 0-5 mM EGTA, 0-25 mM Mg²SOsup>4, 0.1 mM GTP. Drug binding is determined by equilibrium dialysis using concentrations of parbendazole between 0.1 μM and 4 μM, and 2% (v/v) DMF (dimethyl formamide) as a carrier. Equilibrium is achieved by constant stirring for 2 h at 26°C, bovine serum albumin being used as a standard. 200 μL aliquots are counted in PCS in a 25-200B liquid scintillation counter^[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Assay ^[2]	Vero cells, an established cell line derived from monkey kidney are seeded in DMEM supplemented with 10% (v/v) foetal calf serum onto glass coverslips in multiwell dishes. They are allowed to settle, and spread for 2-5 h in a humid atmosphere at 37°C. After this time the medium is changed to DMEM containing 2, 10 or 20 μM parbendazole and 1% (v/v) DMSO controls contained 1 % (v/v) DMSO or had no additions^[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References	[1]. Lo YC, et al. Computational Cell Cycle Profiling of Cancer Cells for Prioritizing FDA-Approved Drugs with Repurposing Potential. Sci Rep. 2017 Sep 12;7(1):11261. [Content Brief] [2]. Havercroft JC, et al. Binding of parbendazole to tubulin and its influence on microtubules in tissue-culture cells as revealed by immunofluorescence microscopy. J Cell Sci. 1981 Jun;49:195-204. [Content Brief] [3]. Foster KE, et al. A mutant beta-tubulin confers resistance to the action of benzimidazole-carbamate microtubule inhibitors both in vivo and in vitro. Eur J Biochem. 1987 Mar 16;163(3):449-55. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.0438 mL	20.2192 mL	40.4384 mL	101.0959 mL
	5 mM	0.8088 mL	4.0438 mL	8.0877 mL	20.2192 mL
	10 mM	0.4044 mL	2.0219 mL	4.0438 mL	10.1096 mL
	15 mM	0.2696 mL	1.3479 mL	2.6959 mL	6.7397 mL

Parbendazole Related Classifications

Infection

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Parbendazole14255-87-9SKF 29044SKF29044SKF-29044Microtubule/TubulinParasiteInhibitorinhibitorinhibit

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Parbendazole (Synonyms: SKF 29044)

Customer Review

Other Forms of Parbendazole:

Parbendazole Related Antibodies

6 Publications Citing Use of MCE Parbendazole

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•Related Screening Libraries:

•Related Small Molecules:

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View All Parasite Isoform Specific Products:

Biological Activity

Protocol

Purity & Documentation

References

Customer Review

Parbendazole Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Parbendazole Related Classifications

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