Puromycin (Synonyms: CL13900)

Name: Puromycin
Brand: MedChemExpress
SKU: HY-B1743
Rating: 5.0 (241 reviews)

Cat. No.: HY-B1743 Purity: 99.55%: FAQs
Data Sheet
SDS

COA
Handling Instructions Technical Support

Puromycin dihydrochloride is the dihydrochloride salt of puromycin. Puromycin is an aminoglycoside antibiotic that inhibits protein synthesis.

For research use only. We do not sell to patients.

Puromycin Chemical Structure

CAS No. : 53-79-2

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	Get quote
50 mg	Get quote

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Based on 241 publication(s) in Google Scholar

Other Forms of Puromycin:

238 Publications Citing Use of MCE Puromycin

: Puromycin purchased from MedChemExpress. Usage Cited in: Autophagy. 2025 Jan 6. [Abstract]; Wild-type or mutated A549 cells subjected to the indicated treatments were stably transfected with puromycin dihydrochloride-inducible stubRFP-sensGFP-LC3 at an MOI of 5 (scale bar: 10 μm).

: Puromycin purchased from MedChemExpress. Usage Cited in: Mol Cell. 2023 Nov 24:S1097-2765(23)00911-5. [Abstract]; Global protein synthesis analyzed by puromycin dihydrochloride (4 d) labeling assay upon METTL14 knockdown in HeLa cells. Silencing of EIF3A serves as a positive control.

: Puromycin purchased from MedChemExpress. Usage Cited in: Mol Cell. 2023 Nov 24:S1097-2765(23)00911-5. [Abstract]; Global protein synthesis analyzed by puromycin dihydrochloride (4 d) labeling assay upon IGF2BP1-3 knockdown in HeLa cells.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Puromycin dihydrochloride is the dihydrochloride salt of puromycin. Puromycin is an aminoglycoside antibiotic that inhibits protein synthesis.

IC₅₀ & Target

Aminoglycoside

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	300 nM Compound: 5	Cytotoxicity against human A549 cells assessed as decrease in cell viability after 48 hrs Cytotoxicity against human A549 cells assessed as decrease in cell viability after 48 hrs	[PMID: 30418763]
HEK293	CC₅₀	0.23 μM Compound: Puromycin	Cytotoxicity against human HEK293 cells assessed as reduction in cell viability measured after 48 hrs by resazurin assay Cytotoxicity against human HEK293 cells assessed as reduction in cell viability measured after 48 hrs by resazurin assay	[PMID: 33479668]
HEK293	IC₅₀	0.35 nM Compound: Puromycin	Cytotoxicity against HEK293 cells by alamar blue assay Cytotoxicity against HEK293 cells by alamar blue assay	[PMID: 27212070]
HEK293	IC₅₀	0.36 μM Compound: Puromycin	Growth inhibition of HEK293 cells after 72 hrs by PrestoBlue staining based fluorescence assay Growth inhibition of HEK293 cells after 72 hrs by PrestoBlue staining based fluorescence assay	[PMID: 28001067]
HEK293	IC₅₀	0.361 μM Compound: Puromycin	Cytotoxicity against HEK293 cells assessed as cell viability after 72 hrs by resazurin-based plate reader analysis Cytotoxicity against HEK293 cells assessed as cell viability after 72 hrs by resazurin-based plate reader analysis	[PMID: 26651537]
HEK293	IC₅₀	0.4 μM Compound: Puromycin	Cytotoxicity against human HEK293 cells after 72 hrs by resazurin dye based fluorescence assay Cytotoxicity against human HEK293 cells after 72 hrs by resazurin dye based fluorescence assay	[PMID: 29969262]
HEK293	IC₅₀	0.42 μM Compound: Puromycin	Cytotoxicity against HEK239 cells after 72 hrs by Alamar blue assay Cytotoxicity against HEK239 cells after 72 hrs by Alamar blue assay	[PMID: 20558060]
HEK293	IC₅₀	0.45 μM Compound: Puromycin	Cytotoxicity against HEK293 cells after 72 hrs by resazurin dye based assay Cytotoxicity against HEK293 cells after 72 hrs by resazurin dye based assay	[PMID: 29236492]
HEK293	IC₅₀	0.46 μM Compound: Puromycin	Cytotoxicity against HEK293 cells assessed as inhibition of cell growth after 72 hrs by alamar blue assay Cytotoxicity against HEK293 cells assessed as inhibition of cell growth after 72 hrs by alamar blue assay	[PMID: 29533611]
HEK293	IC₅₀	0.46 μM Compound: Puromycin	Cytotoxicity against HEK293 cells assessed as cell growth inhibition after 72 hrs by Alamar blue assay Cytotoxicity against HEK293 cells assessed as cell growth inhibition after 72 hrs by Alamar blue assay	[PMID: 28774427]
HEK293	IC₅₀	0.6645 μM Compound: Puromycin	Cytotoxicity against HEK293 cells assessed as inhibition of cell growth incubated for 72 hrs by alamar blue dye based fluorescence assay Cytotoxicity against HEK293 cells assessed as inhibition of cell growth incubated for 72 hrs by alamar blue dye based fluorescence assay	[PMID: 36799121]
HEK293	IC₅₀	0.694 μM Compound: Puromycin	Cytotoxicity against HEK293 cells after 72 hrs by resazurin dye-based fluorescence assay Cytotoxicity against HEK293 cells after 72 hrs by resazurin dye-based fluorescence assay	[PMID: 30865443]
HEK293	IC₅₀	0.81 μM Compound: Puromycin	Cytotoxicity against human HEK293 cells assessed as reduction in cell viability after 72 hrs by resazurin dye based assay Cytotoxicity against human HEK293 cells assessed as reduction in cell viability after 72 hrs by resazurin dye based assay	[PMID: 33105995]
HEK293	IC₅₀	350.5 nM Compound: Puromycin	Cytotoxicity against HEK293 cells assessed as growth inhibition after 72 hrs by alamar blue viability assay Cytotoxicity against HEK293 cells assessed as growth inhibition after 72 hrs by alamar blue viability assay	[PMID: 27060763]
HEK293	CC₅₀	500.1 nM Compound: Puromycin	Cytotoxicity against HEK293 cells after 72 hrs by resazurin dye based assay Cytotoxicity against HEK293 cells after 72 hrs by resazurin dye based assay	[PMID: 30537832]
HEK-293T	CC₅₀	0.42 μM Compound: Puromycin	Cytotoxicity against human HEK293T cells assessed as decrease in cell viability after 72 hrs by CellTiter-Glo reagent based luminescence assay Cytotoxicity against human HEK293T cells assessed as decrease in cell viability after 72 hrs by CellTiter-Glo reagent based luminescence assay	[PMID: 29469575]
HepG2	IC₅₀	0.23 μM Compound: puromycin	Growth inhibition of human HepG2 cells after 72 hrs by MTT assay Growth inhibition of human HepG2 cells after 72 hrs by MTT assay	[PMID: 18313307]
HepG2	CC₅₀	0.83 μM Compound: Puromycin	Cytotoxicity against human HepG2 cells assessed as decrease in cell viability after 72 hrs by CellTiter-Glo reagent based luminescence assay Cytotoxicity against human HepG2 cells assessed as decrease in cell viability after 72 hrs by CellTiter-Glo reagent based luminescence assay	[PMID: 29469575]
HepG2	IC₅₀	2.1 x 10^-1 μM Compound: puromycin	Growth inhibition of human HepG2 cells after 72 hrs by SRB assay Growth inhibition of human HepG2 cells after 72 hrs by SRB assay	[PMID: 18512984]
HepG2	IC₅₀	83.93 μM Compound: puromycin	Growth inhibition of doxorubicin resistant human HepG2 cells after 72 hrs by MTT assay Growth inhibition of doxorubicin resistant human HepG2 cells after 72 hrs by MTT assay	[PMID: 18313307]
HL-60	EC₅₀	0.055 μM Compound: 1	Antiproliferative activity against human HL60 cells after 72 hrs by MTT assay Antiproliferative activity against human HL60 cells after 72 hrs by MTT assay	[PMID: 28803047]
J774.A1	IC₅₀	10 μM Compound: Puromycin	Cytotoxicity against mouse J774A1 cells by trypan blue exclusion assay Cytotoxicity against mouse J774A1 cells by trypan blue exclusion assay	[PMID: 22934636]
K562	IC₅₀	0.22 μM Compound: puromycin	Growth inhibition of human K562 cells after 72 hrs by SRB assay Growth inhibition of human K562 cells after 72 hrs by SRB assay	[PMID: 18313307]
K562	IC₅₀	26.83 μM Compound: puromycin	Growth inhibition of doxorubicin-resistant human K562 cells after 72 hrs by SRB assay Growth inhibition of doxorubicin-resistant human K562 cells after 72 hrs by SRB assay	[PMID: 18313307]
KB 3-1	IC₅₀	0.21 μM Compound: puromycin	Growth inhibition of human KB3-1 cells after 72 hrs by MTT assay Growth inhibition of human KB3-1 cells after 72 hrs by MTT assay	[PMID: 18313307]
KB-V1	IC₅₀	79.3 μM Compound: puromycin	Growth inhibition of human KB V1 cells after 72 hrs by MTT assay Growth inhibition of human KB V1 cells after 72 hrs by MTT assay	[PMID: 18313307]
MDA-MB-231	CC₅₀	0.48 μM Compound: Puromycin	Cytotoxicity against human MDA-MB-231 cells assessed as growth inhibition after 72 hrs by CellTitre-Glo luminescent assay Cytotoxicity against human MDA-MB-231 cells assessed as growth inhibition after 72 hrs by CellTitre-Glo luminescent assay	[PMID: 28523103]
MIA PaCa-2	CC₅₀	0.2 μM Compound: Puromycin	Cytotoxicity against human MIAPaCa2 cells assessed as growth inhibition after 72 hrs by CellTitre-Glo luminescent assay Cytotoxicity against human MIAPaCa2 cells assessed as growth inhibition after 72 hrs by CellTitre-Glo luminescent assay	[PMID: 28523103]
MOLT-4	EC₅₀	0.17 μM Compound: 1	Antiproliferative activity against human MOLT4 cells after 72 hrs by MTT assay Antiproliferative activity against human MOLT4 cells after 72 hrs by MTT assay	[PMID: 28803047]
PC-3	IC₅₀	300 nM Compound: 5	Cytotoxicity against human PC3 cells assessed as decrease in cell viability after 48 hrs Cytotoxicity against human PC3 cells assessed as decrease in cell viability after 48 hrs	[PMID: 30418763]
SU-DHL10	CC₅₀	0.11 μM Compound: Puromycin	Cytotoxicity against human SU-DHL10 cells assessed as growth inhibition after 72 hrs by CellTitre-Glo luminescent assay Cytotoxicity against human SU-DHL10 cells assessed as growth inhibition after 72 hrs by CellTitre-Glo luminescent assay	[PMID: 28523103]
Vero	CC₅₀	2.8 μM Compound: 1	Cytotoxicity against African green monkey Vero cells assessed as reduction in cell proliferation after 72 hrs by MTT assay Cytotoxicity against African green monkey Vero cells assessed as reduction in cell proliferation after 72 hrs by MTT assay	[PMID: 28803047]

In Vitro

Puromycin blocks protein synthesis after aminoacyl-sRNA formation, and at the same time it leads to the accumulation of small peptides. Both of these effects appear to be due to the splitting of ribosome-bound peptidyl-sRNA,4 which results in release of incomplete peptide chains.^[1]. Puromycin, an analog of the 3' end of aminoacyl-tRNA, causes premature termination of translation by being linked non-specifically to growing polypeptide chains. Puromycin has two modes of inhibitory action. The first is by acting as an acceptor substrate which attacks peptidyl-tRNA in the P site to form a nascent peptide. The second is by competing with aminoacyl-tRNA for binding to the A' site^[2]. When used in minimal amounts, puromycin incorporation in neosynthesized proteins reflects directly the rate of mRNA translation in vitro. Puromycin immunodetection is an advantageous alternative to radioactive amino acid labeling. It allows the direct evaluation of translation activity in single cells by immunofluorescence microscopy and in heterogenous populations of cells by fluorescenceactivated cell sorting^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

471.51

Formula

C₂₂H₂₉N₇O₅

CAS No.

53-79-2

Appearance

Solid

Color

White to off-white

SMILES

OC[C@@H]1[C@@H](NC([C@@H](N)CC2=CC=C(OC)C=C2)=O)[C@H]([C@H](N3C=NC4=C3N=CN=C4N(C)C)O1)O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (212.08 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 1 mg/mL (2.12 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.1208 mL	10.6042 mL	21.2085 mL
5 mM	0.4242 mL	2.1208 mL	4.2417 mL
10 mM	0.2121 mL	1.0604 mL	2.1208 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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Concentration _(final)

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.55%

Select Batch:

Data Sheet (276 KB) SDS (419 KB)

COA (253 KB) HNMR (501 KB) LCMS (97 KB)

Handling Instructions (2659 KB)

References

[1]. Nathans D, et al. Puromycin inhibition of protein synthesis: incorporation of puromycin intopeptide chains. Proc Natl Acad Sci U S A. 1964 Apr;51:585-92. [Content Brief]

[2]. Miyamoto-Sato E, et al. Specific bonding of puromycin to full-length protein at the C-terminus. Nucleic Acids Res. 2000 Mar 1;28(5):1176-82. [Content Brief]

[3]. Schmidt EK, et al. SUnSET, a nonradioactive method to monitor protein synthesis. Nat Methods. 2009 Apr;6(4):275-7. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	2.1208 mL	10.6042 mL	21.2085 mL	53.0211 mL
DMSO	5 mM	0.4242 mL	2.1208 mL	4.2417 mL	10.6042 mL
	10 mM	0.2121 mL	1.0604 mL	2.1208 mL	5.3021 mL
	15 mM	0.1414 mL	0.7069 mL	1.4139 mL	3.5347 mL
	20 mM	0.1060 mL	0.5302 mL	1.0604 mL	2.6511 mL
	25 mM	0.0848 mL	0.4242 mL	0.8483 mL	2.1208 mL
	30 mM	0.0707 mL	0.3535 mL	0.7069 mL	1.7674 mL
	40 mM	0.0530 mL	0.2651 mL	0.5302 mL	1.3255 mL
	50 mM	0.0424 mL	0.2121 mL	0.4242 mL	1.0604 mL
	60 mM	0.0353 mL	0.1767 mL	0.3535 mL	0.8837 mL
	80 mM	0.0265 mL	0.1326 mL	0.2651 mL	0.6628 mL
	100 mM	0.0212 mL	0.1060 mL	0.2121 mL	0.5302 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Puromycin Related Classifications

Infection

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Puromycin53-79-2CL13900CL 13900CL-13900BacterialAntibioticParasiteInhibitorinhibitorinhibit

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Puromycin (Synonyms: CL13900)

Customer Review

Other Forms of Puromycin:

Puromycin Related Antibodies

238 Publications Citing Use of MCE Puromycin

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Puromycin Related Antibodies

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Complete Stock Solution Preparation Table

Puromycin Related Classifications

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