2. Apoptosis
  3. Bcl-2 Family
  4. Pyridoclax

Pyridoclax  (Synonyms: MR-29072)

Cat. No.: HY-12527 Purity: 99.95%
COA Handling Instructions

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Pyridoclax is a potential Mcl-1 inhibitor.

For research use only. We do not sell to patients.

Pyridoclax Chemical Structure

Pyridoclax Chemical Structure

CAS No. : 1651890-44-6

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10 mM * 1 mL in DMSO
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10 mM * 1 mL in DMSO USD 88 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

Pyridoclax Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Pyridoclax

View All Bcl-2 Family Isoform Specific Products:

View All Isoforms
Bcl-2 Bcl-xL Bcl-W Mcl-1 Bfl-1 Bcl-B Bax Bak Bim

  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

Pyridoclax is a potential Mcl-1 inhibitor.

IC50 & Target

Mcl-1

 

In Vitro

Pyridoclax directly binds to Mcl-1. Without cytotoxic activity when administered as a single agent, Pyridoclax induces apoptosis in combination with Bcl-xL-targeting siRNA or with ABT-737 in ovarian, lung, and mesothelioma cancer cells[1]. Pyridoclax directly binds to Mcl-1, and hence sensitizes ovarian carcinoma cells to Bcl-xL-targeting strategies. Pyridoclax induces apoptosis in ovarian, and also in lung, and mesothelioma cancer cells when it is administrated in combination with Bcl-xL-targeting siRNA or Bcl-xL targeting molecules such as ABT-737 or its orally available derivative ABT-263[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Pyridoclax Related Antibodies
Molecular Weight

426.51

Formula

C29H22N4
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CC1=CC(C2=NC=C(C3=CC=CN=C3)C=C2/C=C/C4=CC=CC=C4)=CN=C1C5=CC=CN=C5
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 20 mg/mL (46.89 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.3446 mL 11.7231 mL 23.4461 mL
5 mM 0.4689 mL 2.3446 mL 4.6892 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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C2

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V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

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g

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(per animal)

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.95%

COA (254 KB) LCMS (118 KB)

Handling Instructions (2659 KB)
References
Kinase Assay
[1]

For donor saturating assays, Hela cells are seeded on 12-well plates and transfected with 200 ng/well of plasmid pRluc-BimL coding for BRET donor and an increasing quantity of BRET acceptor plasmids peYFP-Mcl-1 (or with pCMV-Mcl-1 as a control). Twenty-four hours after transfection, cells are trypsinized, reseeded into white flat bottom 96-well plates, and incubated for another day before measurements. A single donor/acceptor ratio (200/800) is used to carry out the drug treatment assay. After reseeding, cells are then subject to a 16 h Pyridoclax treatment. Light emission at 485 and 530 nm is measured consecutively using the Mithras fluorescence-luminescence detector LB 940 after adding the luciferase substrate, coelenterazine H, at a final concentration of 5 μM. BRET ratios are calculated[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.3446 mL 11.7231 mL 23.4461 mL 58.6153 mL
5 mM 0.4689 mL 2.3446 mL 4.6892 mL 11.7231 mL
10 mM 0.2345 mL 1.1723 mL 2.3446 mL 5.8615 mL
15 mM 0.1563 mL 0.7815 mL 1.5631 mL 3.9077 mL
20 mM 0.1172 mL 0.5862 mL 1.1723 mL 2.9308 mL
25 mM 0.0938 mL 0.4689 mL 0.9378 mL 2.3446 mL
30 mM 0.0782 mL 0.3908 mL 0.7815 mL 1.9538 mL
40 mM 0.0586 mL 0.2931 mL 0.5862 mL 1.4654 mL
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Pyridoclax Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Pyridoclax1651890-44-6MR-29072MR29072MR 29072Bcl-2 FamilyInhibitorinhibitorinhibit

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