2. GPCR/G Protein Neuronal Signaling
  3. Dopamine Receptor
  4. SKF-82958 hydrobromide

SKF-82958 hydrobromide  (Synonyms: (±)-SKF-82958 hydrobromide; Chloro-APB hydrobromide)

Cat. No.: HY-10435A Purity: 99.82%
COA Handling Instructions

Molarity Calculator

SKF-82958 ((±)-SKF 82958) hydrobromide is a dopamine D1 receptor full agonist (K0.5=4 nM), displays selective for D1 over D2 receptors (K0.5=73 nM). SKF-82958 hydrobromide induces dopamine D1 receptor-dependent adenylate cyclase activity in rat striatal membranes (EC50=491 nM).

For research use only. We do not sell to patients.

SKF-82958 hydrobromide Chemical Structure

SKF-82958 hydrobromide Chemical Structure

CAS No. : 74115-01-8

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 131 In-stock
Solution
10 mM * 1 mL in DMSO USD 131 In-stock
Solid
5 mg USD 119 In-stock
10 mg USD 192 In-stock
25 mg USD 384 In-stock
50 mg USD 696 In-stock
100 mg USD 1260 In-stock
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Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of SKF-82958 hydrobromide:

SKF-82958 hydrobromide Related Antibodies

Top Publications Citing Use of Products

2 Publications Citing Use of MCE SKF-82958 hydrobromide

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Dopamine Receptor D1 Receptor D2 Receptor D3 Receptor D4 Receptor D5 Receptor

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

SKF-82958 ((±)-SKF 82958) hydrobromide is a dopamine D1 receptor full agonist (K0.5=4 nM), displays selective for D1 over D2 receptors (K0.5=73 nM). SKF-82958 hydrobromide induces dopamine D1 receptor-dependent adenylate cyclase activity in rat striatal membranes (EC50=491 nM)[1].

IC50 & Target

D1 Receptor

 

In Vivo

SKF-82958 ((±)-SKF 82958) hydrobromide (0.003-0.1 mg/kg; i.p.) results in dose-dependent increases in responding on the SKF-82958 appropriate lever, with full substitution following administration of the SKF-82958 hydrobromide training dose 0.03 mg/kg. Increasing the dose of SKF-82958 hydrobromide also results in a significant reduction in response rate[2].
SKF-82958 hydrobromide (0.5-2.0 mg/kg; i.p) significantly suppresses pilocarpine-induced jaw movements[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male Sprague-Dawley rats[3]
Dosage: 0.5-2.0 mg/kg
Administration: I.p
Result: Significantly reduced the number of tremulous jaw movements induced by 4.0 mg/kg pilocarpine.
Molecular Weight

410.73

Formula

C19H21BrClNO2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

OC1=C(O)C=C2C(C3=CC=CC=C3)CN(CC=C)CCC2=C1Cl.[H]Br
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (243.47 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4347 mL 12.1734 mL 24.3469 mL
5 mM 0.4869 mL 2.4347 mL 4.8694 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (6.09 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (6.09 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.82%

COA (255 KB) LCMS (88 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.4347 mL 12.1734 mL 24.3469 mL 60.8672 mL
5 mM 0.4869 mL 2.4347 mL 4.8694 mL 12.1734 mL
10 mM 0.2435 mL 1.2173 mL 2.4347 mL 6.0867 mL
15 mM 0.1623 mL 0.8116 mL 1.6231 mL 4.0578 mL
20 mM 0.1217 mL 0.6087 mL 1.2173 mL 3.0434 mL
25 mM 0.0974 mL 0.4869 mL 0.9739 mL 2.4347 mL
30 mM 0.0812 mL 0.4058 mL 0.8116 mL 2.0289 mL
40 mM 0.0609 mL 0.3043 mL 0.6087 mL 1.5217 mL
50 mM 0.0487 mL 0.2435 mL 0.4869 mL 1.2173 mL
60 mM 0.0406 mL 0.2029 mL 0.4058 mL 1.0145 mL
80 mM 0.0304 mL 0.1522 mL 0.3043 mL 0.7608 mL
100 mM 0.0243 mL 0.1217 mL 0.2435 mL 0.6087 mL
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SKF-82958 hydrobromide Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

SKF-8295874115-01-8(±)-SKF-82958Chloro-APBSKF82958SKF 82958Dopamine ReceptorjawmovementsdiscriminativestimulusantiparkinsonianInhibitorinhibitorinhibit

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