SMER28

Name: SMER28
Brand: MedChemExpress
SKU: HY-100200
Rating: 4.5 (1 reviews)

Cat. No.: HY-100200 Purity: 99.92%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

SMER28 is a positive regulator of autophagy acting via an mTOR-independent mechanism. SMER28 prevents the accumulation of amyloid beta peptide.

For research use only. We do not sell to patients.

SMER28 Chemical Structure

CAS No. : 307538-42-7

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 62	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 62	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 57	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 92	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 185	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 296	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 475	In-stock	Estimated Time of Arrival: December 31
200 mg	USD 760	In-stock	Estimated Time of Arrival: December 31
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1 g		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

1 Publications Citing Use of MCE SMER28

•Neuropsych Dis Treat. 2021 Feb 3;17:297-304. [Abstract]

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Purity & Documentation
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Description

SMER28 is a positive regulator of autophagy acting via an mTOR-independent mechanism. SMER28 prevents the accumulation of amyloid beta peptide.

In Vitro

SMER28 (5-200 μM; 24 hours) shows a dose dependent decline of cell viability^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[4]

Cell Line:	MMS1 cells
Concentration:	5, 25, 50, 75, 100, 150, 200 μM
Incubation Time:	24 hours
Result:	Showed a dose dependent decline of cell viability.

In Vivo

SMER28 (15-65 mg/kg; i.h.; daily, two days before irradiation and during the three days of irradiation) significantly protects against post-irradiation weight loss and enhances survival of mice at 65 mg/kg^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	14 to 16 weeks male mice (Balb/c)^[5]
Dosage:	15, 65 mg/kg
Administration:	Subcutaneous injection; two days before irradiation and during the three days of irradiation (total 5 days)
Result:	Significantly protected against post-irradiation weight loss and enhanced survival of mice at 65 mg/kg.

Molecular Weight

264.12

Formula

C₁₁H₁₀BrN₃

CAS No.

307538-42-7

Appearance

Solid

Color

White to off-white

SMILES

C=CCNC1=C2C=C(Br)C=CC2=NC=N1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : ≥ 32 mg/mL (121.16 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.7862 mL	18.9308 mL	37.8616 mL
5 mM	0.7572 mL	3.7862 mL	7.5723 mL
10 mM	0.3786 mL	1.8931 mL	3.7862 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.47 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (9.47 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (9.47 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.92%

Select Batch:

Data Sheet (277 KB) SDS (476 KB)

COA (250 KB) LCMS (93 KB)

Handling Instructions (2659 KB)

References

[1]. Renna M et al. Chemical inducers of autophagy that enhance the clearance of mutant proteins in neurodegenerative diseases. J Biol Chem. 2010 Apr 9;285(15):11061-7. [Content Brief]

[2]. Nekova TS, et al. Small molecule enhancers of rapamycin induce apoptosis in myeloma cells via GSK3A/Bpreferentially within a protective bone marrow microenvironment. Br J Haematol. 2014 Oct;167(2):272-4. [Content Brief]

[3]. Shen D et al. Novel cell- and tissue-based assays for detecting misfolded and aggregated protein accumulation within aggresomes and inclusion bodies. Cell Biochem Biophys. 2011 Jul;60(3):173-85. [Content Brief]

[4]. Koukourakis MI, et al. SMER28 is a mTOR-independent small molecule enhancer of autophagy that protects mouse bone marrow and liver against radiotherapy. Invest New Drugs. 2018 Oct;36(5):773-781. [Content Brief]

[5]. Tian Y et al. A small-molecule enhancer of autophagy decreases levels of Abeta and APP-CTF via Atg5-dependent autophagy pathway. FASEB J. 2011 Jun;25(6):1934-42. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.7862 mL	18.9308 mL	37.8616 mL	94.6539 mL
	5 mM	0.7572 mL	3.7862 mL	7.5723 mL	18.9308 mL
	10 mM	0.3786 mL	1.8931 mL	3.7862 mL	9.4654 mL
	15 mM	0.2524 mL	1.2621 mL	2.5241 mL	6.3103 mL
	20 mM	0.1893 mL	0.9465 mL	1.8931 mL	4.7327 mL
	25 mM	0.1514 mL	0.7572 mL	1.5145 mL	3.7862 mL
	30 mM	0.1262 mL	0.6310 mL	1.2621 mL	3.1551 mL
	40 mM	0.0947 mL	0.4733 mL	0.9465 mL	2.3663 mL
	50 mM	0.0757 mL	0.3786 mL	0.7572 mL	1.8931 mL
	60 mM	0.0631 mL	0.3155 mL	0.6310 mL	1.5776 mL
	80 mM	0.0473 mL	0.2366 mL	0.4733 mL	1.1832 mL
	100 mM	0.0379 mL	0.1893 mL	0.3786 mL	0.9465 mL

SMER28 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

SMER28307538-42-7SMER 28SMER-28AutophagyInhibitorinhibitorinhibit

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SMER28

Customer Review

SMER28 Related Antibodies

1 Publications Citing Use of MCE SMER28

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

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Biological Activity

Purity & Documentation

References

Customer Review

SMER28 Related Antibodies

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Dilution Calculator

Complete Stock Solution Preparation Table

SMER28 Related Classifications

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