2. Metabolic Enzyme/Protease
  3. Phospholipase
  4. ST271

ST271 is a potent inhibitor of protein tyrosine kinase (PTK), inhibits phospholipase D activation stimulated by fMet-Leu-Phe and PAF, with IC50s of 6.7 and 9 μM, respectively.

For research use only. We do not sell to patients.

ST271 Chemical Structure

ST271 Chemical Structure

CAS No. : 106392-48-7

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 105 In-stock
Solution
10 mM * 1 mL in DMSO USD 105 In-stock
Solid
1 mg USD 35 In-stock
5 mg USD 70 In-stock
10 mg USD 110 In-stock
50 mg USD 330 In-stock
100 mg USD 530 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

ST271 Related Antibodies

Top Publications Citing Use of Products

View All Phospholipase Isoform Specific Products:

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Phospholipase A PLD Phospholipase C

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

ST271 is a potent inhibitor of protein tyrosine kinase (PTK), inhibits phospholipase D activation stimulated by fMet-Leu-Phe and PAF, with IC50s of 6.7 and 9 μM, respectively.

IC50 & Target

IC50: 6.7 μM (phospholipase D, stimulated by fMet-Leu-Phe), 9 μM (phospholipase D, stimulated by PAF)[3]
In Vitro

ST271 partially inhibits peptide phosphorylation in the membrane preparation and in permeabilized platelets[1]. ST271 (100 μM) causes complete inhibition of formation of inositol phosphates induced by FcγRII cross-linking, but also induces a small (< 30%) but significant inhibition of the response to thrombin and U46619[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

ST271 Related Antibodies
Molecular Weight

272.34

Formula

C16H20N2O2
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(N)/C(C#N)=C/C1=CC(C(C)C)=C(O)C(C(C)C)=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (183.59 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.6719 mL 18.3594 mL 36.7188 mL
5 mM 0.7344 mL 3.6719 mL 7.3438 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (9.18 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (9.18 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 98.00%

COA (247 KB) LCMS (113 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.6719 mL 18.3594 mL 36.7188 mL 91.7970 mL
5 mM 0.7344 mL 3.6719 mL 7.3438 mL 18.3594 mL
10 mM 0.3672 mL 1.8359 mL 3.6719 mL 9.1797 mL
15 mM 0.2448 mL 1.2240 mL 2.4479 mL 6.1198 mL
20 mM 0.1836 mL 0.9180 mL 1.8359 mL 4.5899 mL
25 mM 0.1469 mL 0.7344 mL 1.4688 mL 3.6719 mL
30 mM 0.1224 mL 0.6120 mL 1.2240 mL 3.0599 mL
40 mM 0.0918 mL 0.4590 mL 0.9180 mL 2.2949 mL
50 mM 0.0734 mL 0.3672 mL 0.7344 mL 1.8359 mL
60 mM 0.0612 mL 0.3060 mL 0.6120 mL 1.5300 mL
80 mM 0.0459 mL 0.2295 mL 0.4590 mL 1.1475 mL
100 mM 0.0367 mL 0.1836 mL 0.3672 mL 0.9180 mL
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ST271 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

ST271106392-48-7ST 271ST-271PhospholipaseInhibitorinhibitorinhibit

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