Saccharin 1-methylimidazole

Name: Saccharin 1-methylimidazole
Brand: MedChemExpress
SKU: HY-112060
Rating: 4.5 (1 reviews)

Cat. No.: HY-112060 Purity: 98.66%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Saccharin 1-methylimidazole is an activator for DNA/RNA Synthesis.

For research use only. We do not sell to patients.

Saccharin 1-methylimidazole Chemical Structure

CAS No. : 482333-74-4

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 34	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 34	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 31	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 100	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 150	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 240	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Saccharin 1-methylimidazole is an activator for DNA/RNA Synthesis.

IC₅₀ & Target

Target: DNA/RNA Synthesis^[1]

In Vitro

Exposure to certain concentrations of the entirely different promoters phenobarbital and the calcium and sodium salts of saccharin (22, 23, 25, 29, 38) stimulates calcium-deprived T51B cells to initiate DNA synthesis as rapidly and as effectively, or nearly as effectively, as raising the extracellular calcium concentration to 1.25 mM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

265.29

Formula

C₁₁H₁₁N₃O₃S

CAS No.

482333-74-4

Appearance

Solid

Color

Light yellow to orange

SMILES

O=C(C1=C2C=CC=C1)NS2(=O)=O.CN3C=CN=C3

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (376.95 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : ≥ 100 mg/mL (376.95 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.7695 mL	18.8473 mL	37.6946 mL
5 mM	0.7539 mL	3.7695 mL	7.5389 mL
10 mM	0.3769 mL	1.8847 mL	3.7695 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.42 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (9.42 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (9.42 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 98.66%

Select Batch:

Data Sheet (275 KB) SDS (251 KB)

COA (252 KB) HNMR (157 KB) LCMS (356 KB)

Handling Instructions (2659 KB)

References

[1]. Boynton AL, et al. Stimulation of DNA synthesis in calcium-deprived T51B liver cells by the tumor promoters phenobarbital, saccharin, and 12-O-tetradecanoylphorbol-13-acetate. Cancer Res. 1980 Dec;40(12):4541-5. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	3.7695 mL	18.8473 mL	37.6946 mL	94.2365 mL
	5 mM	0.7539 mL	3.7695 mL	7.5389 mL	18.8473 mL
	10 mM	0.3769 mL	1.8847 mL	3.7695 mL	9.4236 mL
	15 mM	0.2513 mL	1.2565 mL	2.5130 mL	6.2824 mL
	20 mM	0.1885 mL	0.9424 mL	1.8847 mL	4.7118 mL
	25 mM	0.1508 mL	0.7539 mL	1.5078 mL	3.7695 mL
	30 mM	0.1256 mL	0.6282 mL	1.2565 mL	3.1412 mL
	40 mM	0.0942 mL	0.4712 mL	0.9424 mL	2.3559 mL
	50 mM	0.0754 mL	0.3769 mL	0.7539 mL	1.8847 mL
	60 mM	0.0628 mL	0.3141 mL	0.6282 mL	1.5706 mL
	80 mM	0.0471 mL	0.2356 mL	0.4712 mL	1.1780 mL
	100 mM	0.0377 mL	0.1885 mL	0.3769 mL	0.9424 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.