Skp2 Inhibitor C1 (Synonyms: SKPin C1)

Name: Skp2 Inhibitor C1
Brand: MedChemExpress
SKU: HY-16661
Rating: 5.0 (10 reviews)

Cat. No.: HY-16661 Purity: 98.21%: FAQs
Data Sheet SDS COA Handling Instructions

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Skp2 Inhibitor C1 (SKPin C1) is an S-phase kinase-related protein 2 (Skp2) inhibitor with an inhibitory effect on metastatic melanoma cells. Skp2 Inhibitor C1 slows the cell cycle, inhibits cell proliferation, and triggers apoptosis.

For research use only. We do not sell to patients.

Skp2 Inhibitor C1 Chemical Structure

CAS No. : 432001-69-9

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 107	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 107	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 105	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 168	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 320	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 505	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 780	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 10 publication(s) in Google Scholar

8 Publications Citing Use of MCE Skp2 Inhibitor C1

: Skp2 Inhibitor C1 purchased from MedChemExpress. Usage Cited in: Oncol Rep. 2016 Jul;36(1):559-66. [Abstract]; Western blotting is performed to detect the expression of p21, p27 in PC-3-TxR cells and DU145-TxR cells treated with Paclitaxel or Skp2 inhibitors alone or their combination treatment. Paclitaxel or Skp2 inhibitor treatment alone leads to the accumulation of p27 in PC3-TxR or DU145-TxR.

: Skp2 Inhibitor C1 purchased from MedChemExpress. Usage Cited in: Mol Med Rep. 2016 Oct;14(4):3917-24. [Abstract]; BGC 823 human gastric cancer cells (3×10⁵ cells/well) are plated into 48 well plates. After 6 h, the cells are treated with SKPin C1 at concentrations of 1, 5 and 10 μM for 48 h. The expression levels of Skp2 and p27^kip1 are detected using western blot analysis and grey values are calcu-lated.

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Description

In Vitro

Skp2 Inhibitor C1 (10-50 μM; 12 hr) decreases the viability of THP-1, U266 and RPMI 8226 cells^[1].
Skp2 Inhibitor C1 (25 μM) increases p27 protein levels in U266 and RPMI 8226 cells by inhibiting ubiquitination^[1].
Skp2 Inhibitor C1 (25 μM) inhibits cell cycle of U266 and RPMI 8226 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cycle Analysis^[1]

Cell Line:	B lymphocytes, THP-1, U266 and RPMI 8226 cells
Concentration:	0, 5, 10, 25, and 50 μM
Incubation Time:	12 hr, 24 hr, 36 hr, and 48 hr
Result:	Significantly decreased the viability of U266 and RPMI 8226 cells at 10 μM for 12 hours. Did not significantly affect B lymphocyte viability at 50 μM. Decreased THP-1 cell viability at 50 μM for 12 hours.

Cell Viability Assay^[1]

Cell Line:	U266 and RPMI 8226 cells
Concentration:	0, 5, 10, 25, and 50 μM
Incubation Time:	12 hr
Result:	Increased the percentages of U266 and RPMI 8226 cells in the G0/G1 phase, while decreased the percentages in S and G2/M phases.

In Vivo

Skp2 Inhibitor C1 (5 mg/kg and 10 mg/kg; 3 times within 24 h: 24, 5, and 1 h before the test) shows the antidepressant-like effect in mouse models following chronic treatment by using the tail suspension test (TST), forced swimming test (FST), and social interaction test (SIT)^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

465.34

Formula

C₁₈H₁₃BrN₂O₄S₂

CAS No.

432001-69-9

Appearance

Solid

Color

White to yellow

SMILES

S=C(S/1)N(CC2=CN=CC=C2)C(C1=C\C(C=C(Br)C=C3)=C3OCC(O)=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 20.83 mg/mL (44.76 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.1490 mL	10.7448 mL	21.4897 mL
5 mM	0.4298 mL	2.1490 mL	4.2979 mL
10 mM	0.2149 mL	1.0745 mL	2.1490 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (4.47 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.21%

Select Batch:

Data Sheet (280 KB) SDS (393 KB)

COA (257 KB) HNMR (273 KB) LCMS (276 KB)

Handling Instructions (2659 KB)

References

[1]. Yang Y, et al. Skp2 inhibitor SKPin C1 decreased viability and proliferation of multiple myeloma cells and induced apoptosis. Braz J Med Biol Res. 2019;52(5):e8412. [Content Brief]

[2]. Li F, et al. Identification of the antidepressive properties of C1, a specific inhibitor of Skp2, in mice. Behav Pharmacol. 2021 Feb 1;32(1):62-72. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.1490 mL	10.7448 mL	21.4897 mL	53.7242 mL
	5 mM	0.4298 mL	2.1490 mL	4.2979 mL	10.7448 mL
	10 mM	0.2149 mL	1.0745 mL	2.1490 mL	5.3724 mL
	15 mM	0.1433 mL	0.7163 mL	1.4326 mL	3.5816 mL
	20 mM	0.1074 mL	0.5372 mL	1.0745 mL	2.6862 mL
	25 mM	0.0860 mL	0.4298 mL	0.8596 mL	2.1490 mL
	30 mM	0.0716 mL	0.3582 mL	0.7163 mL	1.7908 mL
	40 mM	0.0537 mL	0.2686 mL	0.5372 mL	1.3431 mL

Skp2 Inhibitor C1 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Skp2 Inhibitor C1432001-69-9SKPin C1SKPin C 1SKPin C-1E1/E2/E3 EnzymeE1 activating enzymeE2 conjugating enzymeE3 ligating enzymeUbiquitin activating enzymeUbiquitin conjugating enzymeUbiquitin ligasecell cyclecell proliferationS-phase kinase-relatedInhibitorinhibitorinhibit

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Skp2 Inhibitor C1 (Synonyms: SKPin C1)

Customer Review

Skp2 Inhibitor C1 Related Antibodies

8 Publications Citing Use of MCE Skp2 Inhibitor C1

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•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

Skp2 Inhibitor C1 Related Antibodies

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Dilution Calculator

Complete Stock Solution Preparation Table

Skp2 Inhibitor C1 Related Classifications

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