β-glucuronidase

GUSB

β-glucuronidase is a glycoside hydrolase widely distributed in mammalian tissues, body fluids, microbiota, plants, fish, insects and mollusks. β-glucuronidase belongs to GH family 1, 2, 30, 79, 154 and GH-A. β-glucuronidase can hydrolyze estrogen glucuronides, regulate the enterohepatic circulation and reabsorption of estrogen, and affect estrogen metabolism. β-glucuronidase can also hydrolyze the extracellular sugar residues on the TRPV5 channel, trapping it in the plasma membrane, maintaining calcium channel activity and membrane calcium permeability. This target is related to many disease areas. For example, in cancer, its increased activity is related to the occurrence and development of colorectal cancer, breast cancer, ovarian cancer, etc.; in metabolic diseases, it is related to diabetes and its induced periodontitis; in inflammatory diseases, it involves bacterial peritonitis, bacterial meningitis, pelvic inflammatory disease, etc.; in addition, it is also related to diseases such as endometriosis^[1]^[2]^[3]^[4]^[5]^[6]^[7].

References:

[1]. Wei Y, et al. Gut dysbiosis-derived β-glucuronidase promotes the development of endometriosis. Fertil Steril. 2023 Sep;120(3 Pt 2):682-694. [Content Brief]

[2]. Awolade P, et al. Therapeutic significance of β-glucuronidase activity and its inhibitors: A review. European Journal of Medicinal Chemistry, 2020, 187: 111921.

[3]. Kim DH, et al. Intestinal bacterial beta-glucuronidase activity of patients with colon cancer. Arch Pharm Res. 2001 Dec;24(6):564-7. [Content Brief]

[4]. Chang Q, et al. The beta-glucuronidase klotho hydrolyzes and activates the TRPV5 channel. Science. 2005 Oct 21;310(5747):490-3. [Content Brief]

[5]. Sui Y, et al. The Role of Gut Microbial β-Glucuronidase in Estrogen Reactivation and Breast Cancer. Front Cell Dev Biol. 2021 Aug 12;9:631552. [Content Brief]

[6]. Liu Z, et al. A novel allosteric driver mutation of β-glucuronidase promotes head and neck squamous cell carcinoma progression through STT3B-mediated PD-L1 N-glycosylation. MedComm (2020). 2025 Jan 19;6(2):e70062. [Content Brief]

[7]. Paigen K. Mammalian beta-glucuronidase: genetics, molecular biology, and cell biology. Prog Nucleic Acid Res Mol Biol. 1989;37:155-205. [Content Brief]

β-glucuronidase Related Products (35):

By Effects:	Inhibitors (19) Substrates (8) Chemical (1)

Cat. No.	Product Name	Effect	Purity	Chemical Structure

HY-134453A

D-Saccharic acid 1,4-lactone hydrate	Inhibitor	≥98.0%
D-Saccharic acid 1,4-lactone hydrate is an orally active β-glucuronidase inhibitor (IC₅₀=48.4 μM). D-Saccharic acid 1,4-lactone hydrate can be used as a standard agent compared with novel β-glucuronidase inhibitors. D-Saccharic acid 1,4-lactone hydrate possesses anticarcinogenic, detoxifying, and antioxidant properties.

HY-P2803

Beta-glucuronidase (Escherichia coli)
Beta-glucuronidase is an important lysosomal enzyme involved in the degradation of glucuronate-containing glycosaminoglycan.

HY-D0935A

4-Methylumbelliferyl-β-D-glucuronide hydrate	Substrate	99.92%
4-Methylumbelliferyl-β-D-glucuronide hydrate is a fluorogenic substrat (λ_ex=362 nm, λ_em=445 nm). 4-Methylumbelliferyl-β-D-glucuronide hydrate has potential applications in detecting the activity of β-glucuronidase and the number of Escherichia coli.

HY-103081

β-Glucuronidase-IN-1	Inhibitor	99.05%
β-Glucuronidase-IN-1 is a potent, selective, uncompetitive, and orally active E. coli bacterial β-glucuronidase inhibitor, exhibiting an IC₅₀ and a K_i of 283 nM and 164 nM, respectively.

HY-W039892

4-Nitrophenyl β-D-glucuronide	Substrate	99.13%
4-Nitrophenyl β-D-glucuronide (pNPG_1) is aderivative of 4-Nitrophenol. 4-Nitrophenyl β-D-glucuronide is the chromogenic substrate for β-glucuronidase.

HY-N2998R

Ganoderenic acid A (Standard)	Inhibitor
Carbidopa (monohydrate) (Standard) is the analytical standard of Carbidopa (monohydrate). This product is intended for research and analytical applications. Carbidopa ((S)-(-)-Carbidopa) monohydrate, a peripheral decarboxylase inhibitor, can be used for the research of Parkinson's disease. Carbidopa monohydrate is a selective aryl hydrocarbon receptor (AhR) modulator. Carbidopa monohydrate inhibits pancreatic cancer cell and tumor growth.

HY-119621

Aceglatone	Inhibitor
Aceglatone, an antineoplastic agent, is a β-glucuronidase inhibitor. Aceglatone is a compound that inhibits colon carcinogenesis and prevents the induction of colon cancer by inhibiting the hydrolysis of glucuronides. Aceglatone reduces cell proliferation, induces apoptosis and inhibits DNA synthesis in human colon cancer cells.

HY-137783

Resorufin β-D-glucuronide sodium	Substrate
Resorufin β-D-glucuronide sodium can be used as a substrate for β-glucuronidase.

HY-148044

UNC10201652	Inhibitor	99.33%
UNC10201652 is a potent Loop 1 (L1)-specific gut bacterial β-glucuronidase (GUSs) inhibitor with an IC₅₀ value of 0.117 μM for E. coli GUS. UNC10201652 can block SN-38 glucuronide (HY-126373) processing only in individuals whose fecal gut microbiota is highly abundant in L1 GUS enzymes.

HY-15935B

X-Gluc cyclohexanamine		99.96%
X-Gluc cyclohexanamine is a dye reagent for the detection of β-glucuronidase (GUS), an enzyme produced by E. coli. X-Gluc cyclohexanamine can be used to detect E. coli contamination in food, water and the urinary tract (GUS as a specific detection indicator). X-Gluc cyclohexanamine is also widely used in molecular biology experiments to label and detect the expression of target genes (reacts with the GUS gene, appears blue).

HY-P2803A

Beta-glucuronidase (bovine liver)
Beta-glucuronidase is an important lysosomal enzyme involved in the degradation of glucuronate-containing glycosaminoglycan.

HY-N1733

2′-Hydroxydaidzein	Inhibitor
2′-Hydroxydaidzein is a metabolite. 2′-Hydroxydaidzein inhibits the release of chemical mediator from inflammatory cells. 2′-Hydroxydaidzein significantly inhibits lysozyme and β-glucuronidase release from rat neutrophils, which is stimulated with fMLP/CB, respectively.

HY-W345885

Saccharic acid	Inhibitor	≥98.0%
Saccharic acid is a competitive inhibitor of β-glucuronidase. Saccharic acid considerably retards hydrolysis of the glucuronide of 'l-ortho-hydroxyphenylazo-2-naph-thol' by frozen mouse kidney sections, but has no effect on liver regeneration following damage and on growth in infant mice.

HY-15935C

X-Gluc sodium		≥98.0%
X-Gluc sodium is a dye reagent for the detection of β-glucuronidase (GUS), an enzyme produced by E. coli. X-Gluc sodium can be used to detect E. coli contamination in food, water and the urinary tract (GUS as a specific detection indicator). X-Gluc sodium is also widely used in molecular biology experiments to label and detect the expression of target genes (reacts with the GUS gene, appears blue).

HY-151168

3-Chlorogentisyl alcohol	Inhibitor	99.80%
3-Chlorogentisyl alcohol is a potent E. coli β-glucuronidase inhibitor with an IC₅₀ value of 0.74 μM, an K_i value of 0.58 μM. 3-Chlorogentisyl alcohol shows antiproliferative activity. 3-Chlorogentisyl alcohol has the potential for the research of anti-cancer and anti-inflammatory therapies.

HY-N2998

Ganoderenic acid A	Inhibitor
Ganoderenic acid A is a lanostane-type triterpene. Ganoderenic acid A is a potent inhibitor of β-glucuronidase. Ganoderenic acid A has a potent hepatoprotective effect against CCl4-induced liver injury.

HY-15935

X-Gluc Dicyclohexylamine		99.48%
X-Gluc Dicyclohexylamine is a dye reagent for the detection of β-glucuronidase, an enzyme produced by Escherichia coli. X-Gluc sodium can be used to detect E. coli contamination in food, water and the urinary tract. X-Gluc sodium is also widely used in molecular biology experiments to label and detect the expression of target genes (GUS reporter system).

HY-150976

β-Glucuronidase/hCAII-IN-1	Inhibitor
β-Glucuronidase/hCAII-IN-2 (Compound 12e) is a potent β-glucuronidase and hCA II inhibitor with an IC₅₀ of 440.1 μM and 4.91 μM, respectively.

HY-D1460

Fluorescein Di-β-D-Glucuronide
Fluorescein Di-β-D-Glucuronide, a fluorescent probe, can be utilized to non-invasively image the intestinal bacterial β-glucuronidase activity in nude mice. Fluorescein Di-β-D-Glucuronide-based imaging reveals the in vitro and in vivo activity of intestinal bacterial β-glucuronidase, which would facilitate pharmacodynamic studies of specific bacterial β-glucuronidase inhibitors in animal studies.

HY-119609

Ilicicolin C	Inhibitor
Ilicicolin C exhibits antibacterial activity against Pseudomonas syringae with IC₅₀ of 28.5 µg/mL. Ilicicolin C exhibits weak inhibitory activities against acetylcholinesterase (AChE) and β-glucuronidase with IC₅₀ of 30-43 µg/mL. Ilicicolin C exhibits weak cytoxicity in human lung fibroblasts with IC₅₀ of 64-120 µg/mL. Ilicicolin C affects seed germination and root tip growth of lettuce.

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