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  4. 4-Nitrophenyl β-D-glucuronide

4-Nitrophenyl β-D-glucuronide  (Synonyms: pNPG_1)

Cat. No.: HY-W039892 Purity: 98.02%
COA Handling Instructions

Molarity Calculator

4-Nitrophenyl β-D-glucuronide (pNPG_1) is aderivative of 4-nitrophenol 4-Nitrophenyl β-D-glucuronide is a chromogenic compound and is the chromogenic substrate for β-glucuronidase.

For research use only. We do not sell to patients.

4-Nitrophenyl β-D-glucuronide Chemical Structure

4-Nitrophenyl β-D-glucuronide Chemical Structure

CAS No. : 10344-94-2

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100 mg USD 50 In-stock
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Based on 1 publication(s) in Google Scholar

4-Nitrophenyl β-D-glucuronide Related Antibodies

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Description

4-Nitrophenyl β-D-glucuronide (pNPG_1) is aderivative of 4-nitrophenol 4-Nitrophenyl β-D-glucuronide is a chromogenic compound and is the chromogenic substrate for β-glucuronidase[1].

Molecular Weight

315.23

Formula

C12H13NO9
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

OC([C@H]([C@H]([C@@H]([C@H]1O)O)O)O[C@H]1OC2=CC=C(C=C2)[N+]([O-])=O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility
In Vitro: 

H2O : 250 mg/mL (793.07 mM; Need ultrasonic)

DMSO : 100 mg/mL (317.23 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.1723 mL 15.8614 mL 31.7229 mL
5 mM 0.6345 mL 3.1723 mL 6.3446 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (7.93 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (7.93 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.13%

COA (250 KB) HNMR (259 KB) LCMS (266 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 3.1723 mL 15.8614 mL 31.7229 mL 79.3072 mL
5 mM 0.6345 mL 3.1723 mL 6.3446 mL 15.8614 mL
10 mM 0.3172 mL 1.5861 mL 3.1723 mL 7.9307 mL
15 mM 0.2115 mL 1.0574 mL 2.1149 mL 5.2871 mL
20 mM 0.1586 mL 0.7931 mL 1.5861 mL 3.9654 mL
25 mM 0.1269 mL 0.6345 mL 1.2689 mL 3.1723 mL
30 mM 0.1057 mL 0.5287 mL 1.0574 mL 2.6436 mL
40 mM 0.0793 mL 0.3965 mL 0.7931 mL 1.9827 mL
50 mM 0.0634 mL 0.3172 mL 0.6345 mL 1.5861 mL
60 mM 0.0529 mL 0.2644 mL 0.5287 mL 1.3218 mL
80 mM 0.0397 mL 0.1983 mL 0.3965 mL 0.9913 mL
100 mM 0.0317 mL 0.1586 mL 0.3172 mL 0.7931 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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4-Nitrophenyl β-D-glucuronide Related Classifications

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    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

4-Nitrophenyl β-D-glucuronide10344-94-2pNPG_1OthersChromogenic SubstrateInhibitorinhibitorinhibit

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